Stable Isotope Labeling of Entire <i>Bacillus atrophaeus</i> Spores and Vegetative Cells Using Bioaerosol Mass Spectrometry

Czerwieniec, Gregg; Russell, Scott C.; Tobias, Herbert J.; Pitesky, Maurice; Fergenson, David P.; Steele, Paul T.; Srivastava, Abneesh; Horn, Joseph M.; Frank, Matthias; Gard, Eric E.; Lebrilla, Carlito B.

doi:10.1021/ac0488098

Cited by 51 publications

(61 citation statements)

References 38 publications

(91 reference statements)

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“…Figure 4 shows average mass spectra collected at several different laser energies. The general appearance of the spectra is similar to those shown in previous papers 13,30,31 where most of the peaks have been identified. The prominence of the potassium peak at m/z=39 in the present spectra may be related in part to the fact that it is the only element likely to be encountered with an ionization potential less than the energy of a single 266nm photon (4.34 eV versus 4.66 eV respectively).…”

Section: Fluence Thresholdssupporting

confidence: 85%

Desorption/Ionization Fluence Thresholds and Improved Mass Spectral Consistency Measured Using a Flattop Laser Profile in the Bioaerosol Mass Spectrometry of Single Bacillus Endospores

et al. 2005

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Bioaerosol mass spectrometry (BAMS) is being developed to analyze and identify biological aerosols in real-time. Mass spectra of individual Bacillus endospores were measured here with a bipolar aerosol time-of-flight mass spectrometer in which molecular desorption and ionization were produced using a single laser pulse from a Q-switched, frequency-quadrupled Nd:YAG laser that was modified to have an approximately flattop profile. The flattened laser profile allowed the minimum fluence required to desorb and ionize significant numbers of ions from single aerosol particles to be determined. For Bacillus spores this threshold had a mean value of approximately 1 nJ/µm 2 (0.1 J/cm 2 ). Thresholds for individual spores, however, could apparently deviate by 20% or more from the mean. Threshold distributions for clumps of MS2 bacteriophage and bovine serum albumin were subsequently determined. Finally, the flattened profile was observed to increase the reproducibility of single spore mass spectra. This is consistent with the general conclusions of our earlier paper on the fluence * E-mail: frank1@llnl.gov Telephone: 925-423-5068 Fax: 925-424-2778 2 dependence of single spore mass spectra and is particularly significant because it is expected to enable more robust differentiation and identification of single bioaerosol particles.

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Section: Fluence Thresholdssupporting

confidence: 85%

Desorption/Ionization Fluence Thresholds and Improved Mass Spectral Consistency Measured Using a Flattop Laser Profile in the Bioaerosol Mass Spectrometry of Single Bacillus Endospores

et al. 2005

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“…shown the ability to distinguish single Bacillus spores from Bacillus vegetative cells [1]. BAMS analyzes single cells without sample pretreatment or preconcentration [2].…”

Section: B Ioaerosol Mass Spectrometry (Bams) Has Recentlymentioning

confidence: 99%

Improved sensitivity and mass range in time-of-flight bioaerosol mass spectrometry using an electrostatic ion guide

Czerwieniec

Russell

Lebrilla

et al. 2005

J. Am. Soc. Mass Spectrom.

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Bioearosol mass spectrometry (BAMS) analyzes single particles in real time from ambient air, placing strict demands on instrument sensitivity. Modeling of the BAMS reflectron time of flight (TOF) with SIMION revealed design limitations associated with ion transmission and instrument sensitivity at higher masses. Design and implementation of a BAMS linear TOF with electrostatic ion guide and delayed extraction capabilities has greatly increased the sensitivity and mass range relative to the reflectron design. Initial experimental assessment of the new instrument design revealed improved sensitivity at high masses as illustrated when using standard particles of cytochrome C (m/z ϳ 12,000), from which the compound's monomer, dimer (m/z ϳ 24,000) and trimer (m/z ϳ 36,000) were readily detected. (J Am Soc Mass Spectrom 2005, 16, 1866 -1875

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“…It has the ability to distinguish Bacillus spores from vegetative cells on a particle by particle basis using ion peaks under m/z 300 [4], and has demonstrated preliminary species level differentiation of individual Bacillus spores using low mass biomarker peaks [5,6]. In spite of this success, the production, detection, and identification of higher-mass taxonomical biomarker peaks is strongly desired because it would enable more robust differentiation of a broader range of bacterial species.…”

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Parameters contributing to efficient ion generation in aerosol MALDI mass spectrometry

McJimpsey

Jackson

Lebrilla

et al. 2008

J. Am. Soc. Mass Spectrom.

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The Bioaerosol Mass Spectrometry (BAMS) system was developed for the real-time detection and identification of biological aerosols using laser desorption ionization. Greater differentiation of particle types is desired; consequently MALDI techniques are being investigated. The small sample size (ϳ1 m 3 ), lack of substrate, and ability to simultaneously monitor both positive and negative ions provide a unique opportunity to gain new insight into the MALDI process. Several parameters known to influence MALDI molecular ion yield and formation are investigated here in the single particle phase. A comparative study of five matrices (2,6-dihydroxyacetophenone, 2,5-dihydroxybenzoic acid, ␣-cyano-4-hydroxycinnamic acid, ferulic ␣ acid, and sinapinic acid) with a single analyte (angiotensin I) is presented and reveals effects of matrix selection, matrix-to-analyte molar ratio, and aerosol particle diameter. The strongest analyte ion signal is found at a matrix-to-analyte molar ratio of 100:1. At this ratio, the matrices yielding the least and greatest analyte molecular ion formation are ferulic acid and ␣-cyano-␣ 4-hydroxycinnamic acid, respectively. Additionally, a significant positive correlation is found between aerodynamic particle diameter and analyte molecular ion yield for all matrices. SEM imaging of select aerosol particle types reveals interesting surface morphology and structure.

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Stable Isotope Labeling of Entire Bacillus atrophaeus Spores and Vegetative Cells Using Bioaerosol Mass Spectrometry

Cited by 51 publications

References 38 publications

Desorption/Ionization Fluence Thresholds and Improved Mass Spectral Consistency Measured Using a Flattop Laser Profile in the Bioaerosol Mass Spectrometry of Single Bacillus Endospores

Desorption/Ionization Fluence Thresholds and Improved Mass Spectral Consistency Measured Using a Flattop Laser Profile in the Bioaerosol Mass Spectrometry of Single Bacillus Endospores

Improved sensitivity and mass range in time-of-flight bioaerosol mass spectrometry using an electrostatic ion guide

Parameters contributing to efficient ion generation in aerosol MALDI mass spectrometry

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