Stable Isotope Applications in Bone Collagen with Emphasis on Deuterium/Hydrogen Ratios

Topalov, Katarina; Schimmelmann, Arndt; Polly, P. David; Sauer, Peter E.

doi:10.5772/34787

Cited by 3 publications

(3 citation statements)

References 82 publications

(126 reference statements)

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“…Both these results are qualitatively consistent with the well-known effect of heavy isotope enrichment along the food chain 25 . However, the enrichment magnitude is staggering, as normally the enrichment with one trophic level is of the order of 1% for both 13 C/ 12 C and 15 N/ 14 N 26 . This adds additional arguments to questioning in the isotopic context of the old paradigm "you are what you eat" 27,28 .…”

Section: Ultralight C Elegans Proteins Accumulate Heavy Isotopesmentioning

confidence: 99%

Do light eaters live shorter lives? The case of ultralightCaenorhabditis elegans

Zhang,

Gharibi,

Beusch

et al. 2024

Preprint

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The idea that ingesting heavy stable isotopes can increase longevity emerged shortly after the discovery of deuterium in the early 1930s and has been extensively tested since then on animals. Here we present the first experimental evidence for the opposite. Growing C. elegans on bacteria E. coli that are in turn fed on a diet depleted of heavy isotopes of C, H, N and O produced ultralight worms that grow and mature faster but have a shorter lifespan. Based on the differences in expression and solubility of proteins, we established an aging pseudo-time scale. Notably, the newly born ultralight worms appear to be significantly "younger" than their normal counterparts, while at day 10 they are significantly "older". Pathway analysis revealed involvement of mitochondria; analysis of reactive oxygen species (ROS) confirmed significant ROS overproduction in ultralight worms that increases further with age. These findings provide a new modality of affecting the lifespan in this important animal model of human diseases and aging.

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Section: Ultralight C Elegans Proteins Accumulate Heavy Isotopesmentioning

confidence: 99%

Do light eaters live shorter lives? The case of ultralightCaenorhabditis elegans

Zhang,

Gharibi,

Beusch

et al. 2024

Preprint

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“…The resultant isotopic relationships are quite complex but these are analytically calibrated with the modern faunal analogues. Moreover, the hydrogen isotope concentration of bone collagen should be sensitive to the amount and dietary importance of consumed protein, which offers analytical opportunities for reconstructing diet and trophic structure [20] Stable isotope analysis is proved to be a powerful tool for screening human remains and provenance unidentified bodies from a variety of forensic contexts [24,23].…”

Section: Anthropological Analysismentioning

confidence: 99%

Hydrogen isotope

Mishra

Kaur

Vita

et al. 2022

ijhs

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Stable isotope analysis is a valuable tool in forensic investigation. Recently, isotopic investigation is in great trend, Forensic discipline is making use of multi-purpose isotopic profiles along with isotopic landscapes or isoscapes from body tissues. These isotopes help in predicting the geographical location or origin of the unidentified body. Isotope analysis is a commendable and powerful tool for geolocation and can provide investigative leads to the investigating officer as well as forensic experts. This review article basically focuses on the hydrogen isotopes and their applications in forensic science. Being a bio-element, it is ubiquitous and has immense biological and chemical significance. We have discussed the role of hydrogen isotopes in different disciplines of forensic science such as forensic biology, wildlife forensics, forensic anthropology, forensic chemistry and toxicology etc. and the newer advancements that are employed in this discipline for making the analysis more accurate and robust. This field is still in its growing stage and hence, with more advancements, it will provide a great aid in forensic investigation.

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“…In contrast to the numerous and wide-ranging studies on isotopes, variation in bone remodelling across the skeleton has received much less attention [see 4,7,10,11] and our understanding remains equivocal. This is largely because it is incredibly difficult to study bone in vivo [12][13][14][15][16]. Access to skeletal collections of identified individuals is also limited, reducing the number of potential research subjects [11].…”

Section: Introductionmentioning

confidence: 99%

Bone of contention: Intra-element variability in remodelling of human femora based on histomorphometric and isotope analyses

de Gruchy,

Faillace,

Van de Vijver

et al. 2024

PLoS ONE

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The volume of human carbon (δ13C) and nitrogen (δ15N) isotope data produced in archaeological research has increased markedly in recent years. However, knowledge of bone remodelling, its impact on isotope variation, and the temporal resolution of isotope data remains poorly understood. Varied remodelling rates mean different elements (e.g., femur and rib) produce different temporal signals but little research has examined intra-element variability. This study investigates human bone remodelling using osteon population density and the relationship with carbon and nitrogen isotope data at a high resolution, focusing on variation through femoral cross-sections, from periosteal to endosteal surfaces. Results demonstrate considerable differences in isotope values between cross-sectional segments of a single fragment, by up to 1.3‰ for carbon and 1.8‰ for nitrogen, illustrating the need for standardised sampling strategies. Remodelling also varies between bone sections, occurring predominantly within the endosteal portion, followed by the midcortical and periosteal. Therefore, the endosteal portion likely reflects a shorter period of life closer to the time of death, consistent with expectations. By contrast, the periosteal surface provides a longer average, though there were exceptions to this. Results revealed a weak negative correlation between osteon population density and δ15N or δ13C, confirming that remodelling has an effect on isotope values but is not the principal driver. However, a consistent elevation of δ15N and δ13C (0.5‰ average) was found between the endosteal and periosteal regions, which requires further investigation. These findings suggest that, with further research, there is potential for single bone fragments to reconstruct in-life dietary change and mobility, thus reducing destructive sampling.

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Stable Isotope Applications in Bone Collagen with Emphasis on Deuterium/Hydrogen Ratios

Cited by 3 publications

References 82 publications

Do light eaters live shorter lives? The case of ultralightCaenorhabditis elegans

Do light eaters live shorter lives? The case of ultralightCaenorhabditis elegans

Hydrogen isotope

Bone of contention: Intra-element variability in remodelling of human femora based on histomorphometric and isotope analyses

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