Stable Isotope Analysis: Outline of Methodology, and a Review of Studies in Siberia and the Eurasian Steppe

Svyatko, Svetlana V.

doi:10.17746/1563-0110.2016.44.2.047-055

Cited by 9 publications

(3 citation statements)

References 21 publications

(37 reference statements)

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“…This misattribution of species necessitates caution if proteomic studies of pottery residues are used to identify exploitation of a variety of resources, particularly the processing of a secondary resource along with a primary one. For example, it is established that hunter–gatherers and pastoralists inhabiting the Siberian forests and steppes during the Neolithic and Bronze Ages regularly exploited fish, ,, but it is unknown to what extent they used additional resources such as waterfowl and forest birds that provide additional seasonal sources of fats and proteins, and it is unlikely that minor contributions from resources can be accurately identified by a proteomic approach alone. Similarly, extreme caution is to be exercised if proteomic data is used to comment on the historic distribution of species, regional extinction phenomenon, and other geographical distribution factors.…”

Section: Resultsmentioning

confidence: 99%

Novel Deep Eutectic Solvent-Based Protein Extraction Method for Pottery Residues and Archeological Implications

et al. 2022

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Proteomic analysis of absorbed residues is increasingly used to identify the foodstuffs processed in ancient ceramic vessels, but detailed methodological investigations in this field remain rare. Here, we present three interlinked methodological developments with important consequences in paleoproteomics: the comparative absorption and identification of various food proteins, the application of a deep eutectic solvent (DES) for extracting ceramic-bound proteins, and the role of database choice in taxonomic identification. Our experiments with modern and ethnoarcheological ceramics show that DES is generally more effective at extracting ceramic-bound proteins than guanidine hydrochloride (GuHCl), and cereal proteins are absorbed and subsequently extracted and identifiedat least as readily as meat proteins. We also highlight some of the challenges in cross-species proteomics, whereby species that are less well-represented in databases can be attributed an incorrect species-level taxonomic assignment due to interspecies similarities in protein sequence. This is particularly problematic in potentially mixed samples such as cooking-generated organic residues deposited in pottery. Our work demonstrates possible proteomic separation of fishes and birds, the latter of which have so far eluded detection through lipidomic analyses of organic residue deposits in pottery, which has important implications for tracking the exploitation of avian species in various ancient communities around the globe.

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Section: Resultsmentioning

confidence: 99%

Novel Deep Eutectic Solvent-Based Protein Extraction Method for Pottery Residues and Archeological Implications

et al. 2022

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“…That paper also discussed the context and implications of this conclusion, including intensified contacts between Karasuk groups and China, further detailed in later publications (Svyatko 2014(Svyatko , 2016. Subsequent recent research on earlier periods in the area and neighboring regions (Marchenko et al 2015;Motuzaite Matuzeviciute et al 2016;Svyatko et al 2017c) has provided no evidence for any significant contribution of millet to human diet.…”

Section: Introductionmentioning

confidence: 93%

Millet Consumption in Siberia Prior to Mid-Second Millennium Bc? A Review of Recent Developments

2021

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In this paper we discuss recent developments in documenting the spread of millet across the Eurasian steppes. We emphasize that, despite a recent proposal that millet consumption in southern Siberia can be attributed to the Early Bronze Age (i.e., the late third to early second millennium BC), at present there are no direct data for southern Siberia indicating the consumption of millet prior to the Late Bronze Age, from the 14th century BC. We also present in full the combined stable isotope and 14C datasets from the Minusinsk Basin to support this conclusion.

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“…Nonetheless, a partial consumption of freshwater fish can be (cautiously) proposed for at least some of the individuals showing particularly depleted carbon and sulfur isotope ratios. It is important to point out that δ 13 C, δ 15 N, and δ 34 S values in freshwater environments are quite variable (Katzenberg & Weber, 1999;Nehlich, 2015;Privat et al, 2007;Svyatko, 2016;Svyatko, Reimer, & Schulting, 2017). and by environmental data and economic reasons.…”

Section: Dietmentioning

confidence: 99%

Dining in Tuva: Social correlates of diet and mobility in Southern Siberia during the 2nd–4th centuries CE

Milella

Caspari

Laffranchi

et al. 2022

American Journal of Biological Anthropology

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Objectives: Contemporary archeological theory emphasizes the economic and social complexity of Eurasian steppe populations. As a result, old notions of "nomadic" cultures as homogenously mobile and economically simple are now displaced by more nuanced interpretations. Large part of the literature on diet and mobility among Eurasian pastoralists is focused on the Bronze and Iron Ages. The underrepresentation of more recent contexts hampers a full discussion of possible chronological trajectories. In this study we explore diet and mobility at Tunnug1 (Republic of Tuva, 2nd-4th century CE), and test their correlation with social differentiation. Materials and Methods:We compare demographic patterns (by age-at-death and sex) of carbon, nitrogen, and sulfur stable isotope ratios (δ 13 C, δ 15 N, and δ 34 S) among 65 humans and 12 animals from Tunnug1 using nonparametric tests and Bayesian modeling. We then compare isotopic data with data on perimortal skeletal lesions of anthropic origin and funerary variables.Results: Our analyses show that: (1) diet at Tunnug1 was largely based on C 4 plants (likely millet) and animal proteins; (2) few individuals were nonlocals, although their geographic origin remains unclarified; (3) no differences in diet separates individuals based on sex and funerary treatment. In contrast, individuals with perimortal lesions show carbon and nitrogen stable isotope ratios consistent with a diet incorporating a lower consumption of millet and animal proteins.Discussion: Our results confirm the previously described socioeconomic variability of steppe populations, providing at the same time new data about the economic importance of millet in Southern Siberia during the early centuries CE.

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Stable Isotope Analysis: Outline of Methodology, and a Review of Studies in Siberia and the Eurasian Steppe

Cited by 9 publications

References 21 publications

Novel Deep Eutectic Solvent-Based Protein Extraction Method for Pottery Residues and Archeological Implications

Novel Deep Eutectic Solvent-Based Protein Extraction Method for Pottery Residues and Archeological Implications

Millet Consumption in Siberia Prior to Mid-Second Millennium Bc? A Review of Recent Developments

Dining in Tuva: Social correlates of diet and mobility in Southern Siberia during the 2nd–4th centuries CE

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