Stable expression of a fungal laccase protein using transplastomic tobacco

Davarpanah, Seyed Javad; Ahn, Joon-Woo; Ko, Suk Min; Jung, Seo Hee; Park, Youn‐Il; Liu, Jang Ryol; Jeong, Won Joong

doi:10.1007/s11816-012-0225-4

Cited by 11 publications

(6 citation statements)

References 32 publications

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“…In this sense, some reports showed low effect in pigmentation with expression of a laccase gene [63] or severe retardation in plant growing when xylanase was expressed in the chloroplast genome [64]. In contrast, Petersen and Bock [5] found that expression of celluloses showed phenotypes with severe pigmentation loss followed by plant death.…”

Section: Discussionmentioning

confidence: 99%

Stable expression and characterization of a fungal pectinase and bacterial peroxidase genes in tobacco chloroplast

Espinoza-Sánchez

Álvarez-Hernández

Torres-Castillo

et al. 2015

Electronic Journal of Biotechnology

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Background: The high capacity of chloroplast genome response to integrate and express transgenes at high levels makes this technology a good option to produce proteins of interest. This report presents the stable expression of Pectin lyase (PelA gene) and the first stable expression of manganese peroxidase (MnP-2 gene) from the chloroplast genome. Results: pES4 and pES5 vectors were derived from pPV111A plasmid and contain the PelA and MnP-2 synthetic genes, respectively. Both genes are flanked by a synthetic rrn16S promoter and the 3′UTR from rbcL gene. Efficient gene integration into both inverted repeats of the intergenic region between rrn16S and 3′rps′12 was confirmed by Southern blot. Stable processing and expression of the RNA were confirmed by Northern blot analysis. Enzymatic activity was evaluated to detect expression and functionality of both enzymes. In general, mature plants showed more activity than young transplastomic plants. Compared to wild type plants, transplastomic events expressing pectin lyase exhibited enzymatic activity above 58.5% of total soluble protein at neutral pH and 60°C. In contrast, MnP-2 showed high activity at pH 6 with optimum temperature at 65°C. Neither transplastomic plant exhibited an abnormal phenotype. Conclusion: This study demonstrated that hydrolytic genes PelA and MnP-2 could be integrated and expressed correctly from the chloroplast genome of tobacco plants. A whole plant, having~470 g of biomass could feasibly yield 66,676.25 units of pectin or 21,715.46 units of manganese peroxidase. Also, this study provides new information about methods and strategies for the expression of enzymes with industrial value.

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Section: Discussionmentioning

confidence: 99%

Stable expression and characterization of a fungal pectinase and bacterial peroxidase genes in tobacco chloroplast

Espinoza-Sánchez

Álvarez-Hernández

Torres-Castillo

et al. 2015

Electronic Journal of Biotechnology

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“…The results illustrated significant pro-apoptotic and antiproliferation effects depending on the time and concentration of the extracts associated with cytochrome C release to the cytosol, caspases-3, -8, -9 activation, the probability of mitochondrial membrane losing and PARP damaging. Terpenoid has been isolated from L. mongolica using ethanol 77.95% with the extraction temperature is 78.78 •c [37], However Terpenoids should be examined in an in vivo and in vitro model for test their potential to as antidiabetic and antitumor or prevent some disease…”

Section: Terpenesmentioning

confidence: 99%

Bioactive Compounds in <em>Leucocalocybe mongolica</em> Pharmacological and Therapeutic Applications

Haiying¹,

Mt²,

Ah³

2021

Preprint

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Medicinal mushrooms have got much attention from biologists in the last decades. Leucocalocybe mongolica Imai remains one of the famous clinical and edible mushrooms used in traditional Chinese medicine and Mongolian medicine to treat various diseases. It also commonly consumes in Chinese and Mongolian daily dishes. Numerous biochemical components exist in L. mongolica, especially in the fruiting bodies include; polysaccharides, sterol, lectins, laccase, amino acids, terpenes, volatile compounds, and so on. These biomedical components possess a lot of medicinal properties and provide diverse therapeutical effects for human health, such as anti-tumor activity, antiproliferative activity, anti-diabetes properties, hypotensive effect, hepatoprotective effect, antioxidant activity, cardioprotective effect, and so on. However, this review's main objectives are to illustrate some basics about the bioactive components in L. mongolica and its pharmacological effects on the human body. In addition to giving forward some suggestions for future research on this medicinal mushroom.

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“…The expression of β-glucosidase also increased twofold the levels of gibberellic acid precursor (GA 53 ,G A 44 ,G A 19 , and GA 20 ), (GA 1 ) hormone, and catabolite (GA 8 ) and in the case of indolacetic acid and zeatin hormones had more than 200% of increases. Surprisingly the glandular trichomas density resulted up to 10-fold with more production of natural sugar esters that shown to be effective biopesticides against a range of insect species [44] in the β-glucosidase expression plants, they showed 18-fold less aphid and whitefly population by a toxicity increase in exudates of plants; but, there are report when the transplastomic plants that expressed lacasses showed slightly retarded vegetative growth with a light green leaf color may be attributable to copper deficiency induced by ligand chelation related to produced laccase [45]. In plants with xylanases overexpressed, a 60% increase of xylanase (xynA gene) was observed and the accumulation of the fungal enzymes was more than 10-fold higher levels but the transplastomic plants displayed pale-green-to-white leaf color and severely retarded growth [46].…”

Section: Enzymes To Cellulose Degradationmentioning

confidence: 99%

Biotechnological Applications of Plastid Foreign Gene Expression

Sánchez¹,

Torres-Castillo²,

Cruz³

et al. 2018

Plant Growth and Regulation - Alterations to Sustain Unfavorable Conditions

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Chloroplast is responsible for the major metabolic process photosynthesis. These organelles have their own genome and in the last three decades, the chloroplast genome has been broadly studied and manipulated through genetic engineering tools. The transfer of genes into chloroplast provides advantage over the insertion of transgene into nuclear genome, including overexpression of foreign protein, no positional effects, absence of epigenetic effects and uniparental inheritance of the transgenes, the ability to express multiple transgenes in operons and the possibility of eliminate the marker gene after the transformation and integration of the foreign gene. Now more than 100 transgenes have been reported stably integrated into the chloroplast genome including genes encoding enzymes with industrial value, biomaterials, biopharmaceuticals, vaccines and genes with agronomic trails. The chloroplast genetic tools have been implemented in several important crops. So, the chloroplast engineering technology has been positioned as the most important for the production of proteins and metabolites with biotechnological applications.

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Stable expression of a fungal laccase protein using transplastomic tobacco

Cited by 11 publications

References 32 publications

Stable expression and characterization of a fungal pectinase and bacterial peroxidase genes in tobacco chloroplast

Stable expression and characterization of a fungal pectinase and bacterial peroxidase genes in tobacco chloroplast

Bioactive Compounds in <em>Leucocalocybe mongolica</em> Pharmacological and Therapeutic Applications

Biotechnological Applications of Plastid Foreign Gene Expression

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