Stable expression of a bifunctional diterpene synthase in the chloroplast of Chlamydomonas reinhardtii

Zedler, Julie A Z; Gangl, Doris; Hamberger, Björn; Purton, Saul; Robinson, Colin

doi:10.1007/s10811-014-0504-2

Cited by 25 publications

(20 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2). specifically reacting band that has been previously observed [20]. In both A and B the 216 promoter used to drive expression is shown below the blot.…”

Section: Chloroplast Isolation and Fractionation 139mentioning

confidence: 66%

“…Transformants generated with 118 the pASapI vector were analysed by PCR as described in Zedler et al [20]. The same 119 protocol was used for transformants generated with pSRSapI-based constructs.…”

Section: Homoplasmy Analysis Of Transformants By Pcr 116mentioning

confidence: 99%

“…The same 119 protocol was used for transformants generated with pSRSapI-based constructs. The 120 primers FLANK1, rbcL.F (both previously described [20] …”

Section: Homoplasmy Analysis Of Transformants By Pcr 116mentioning

confidence: 99%

“…Further details of 107 the cell line generation were as previously described in Zedler et al [20]. Other than 108 the cell lines generated in this study by transformation with the plasmids as shown in 109 TN72-RP* and served as a negative control for transformants based on the pSRSapI 112 vector.…”

mentioning

confidence: 99%

“…Other than 108 the cell lines generated in this study by transformation with the plasmids as shown in 109 TN72-RP* and served as a negative control for transformants based on the pSRSapI 112 vector. The strain TN72-RP (TN72 transformed with pASapI) [20] was used as a 113 negative control for pASapI based transformants. 114 115…”

mentioning

confidence: 99%

See 4 more Smart Citations

Efficient targeting of recombinant proteins to the thylakoid lumen in Chlamydomonas reinhardtii using a bacterial Tat signal peptide

2016

Self Cite

View full text Add to dashboard Cite

19Interest in the exploitation of microalgae for biotechnological applications has 20 increased over the last decade, and microalgae are now viewed as offering a 21 sustainable alternative to traditionally used host chassis. A number of recombinant 22proteins have been expressed in genetically modified algal strains, with the green alga 23Chlamydomonas reinhardtii being a particularly popular host strain.

show abstract

“…2). specifically reacting band that has been previously observed [20]. In both A and B the 216 promoter used to drive expression is shown below the blot.…”

Section: Chloroplast Isolation and Fractionation 139mentioning

confidence: 66%

Section: Homoplasmy Analysis Of Transformants By Pcr 116mentioning

confidence: 99%

“…The same 119 protocol was used for transformants generated with pSRSapI-based constructs. The 120 primers FLANK1, rbcL.F (both previously described [20] …”

Section: Homoplasmy Analysis Of Transformants By Pcr 116mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Efficient targeting of recombinant proteins to the thylakoid lumen in Chlamydomonas reinhardtii using a bacterial Tat signal peptide

2016

Self Cite

View full text Add to dashboard Cite

show abstract

Engineering microalgae through chloroplast transformation to produce high‐value industrial products

Siddiqui

Wei

Boehm

et al. 2019

Biotech and App Biochem

View full text Add to dashboard Cite

The last few years have seen an ever‐increasing interest in the exploitation of microalgae as an alternative platform to produce high‐value products such as biofuels, industrial enzymes, therapeutic proteins, including antibodies, hormones, and vaccines. Due to some unique attractive features, engineering of the chloroplast genome provides a promising platform for the production of high‐value targets because it allows manipulation of metabolic processes in ways that would be impossible, or at least prohibitively difficult through traditional approaches. Since its initial demonstration in 1988 in Chlamydomonas reinhardtii, genetic tools have been developed, which have made it possible to produce high‐value molecules in different species. However, the commercial application of microalgae as production platform is hindered by many factors like poor biomass, low product yields, and costly downstream processing methodologies. In this review, we discuss the potential of microalgae to use as an alternative production platform for high‐value targets using chloroplast transformation technology.

show abstract

Molecular Farming for the Production of Recombinant Pharmaceutical Proteins in Plants

Fidan,

Secgin

2024

Concepts and Strategies in Plant Sciences

View full text Add to dashboard Cite

Stable expression of a bifunctional diterpene synthase in the chloroplast of Chlamydomonas reinhardtii

Cited by 25 publications

References 23 publications

Efficient targeting of recombinant proteins to the thylakoid lumen in Chlamydomonas reinhardtii using a bacterial Tat signal peptide

Efficient targeting of recombinant proteins to the thylakoid lumen in Chlamydomonas reinhardtii using a bacterial Tat signal peptide

Engineering microalgae through chloroplast transformation to produce high‐value industrial products

Molecular Farming for the Production of Recombinant Pharmaceutical Proteins in Plants

Contact Info

Product

Resources

About