Stable chloroplast transformation of immature scutella and inflorescences in wheat (&amp;lt;italic&amp;gt;Triticum aestivum&amp;lt;/italic&amp;gt; L.)

Cui, Cuiju; Song, Fei; Tan, Yen Kheng; Zou, Xuan; Zhao, Weiqiang; Ma, Fengyun; Liu, Yunyi; Hussain, Javeed; Wang, Yue-Sheng; Yang, Guangxiao; He, Guangyuan

doi:10.1093/abbs/gmr008

Cited by 30 publications

(15 citation statements)

References 52 publications

(70 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the delivered foreign genes could be integrated into the genome of protoplastids along with the embryogenesis into adult chloroplasts. Our results are in agreement with Cuiju et al (2011) who successfully managed to achieve a stable chloroplast transformation of immature scutella of wheat.…”

Section: Discussionsupporting

confidence: 92%

Plastid Transformation of Wheat Cultivar Using Plastid Expression Cassette Carrying Nitrogen Fixation Genes

2016

Egypt. J. Bot.

View full text Add to dashboard Cite

HLOROPLAST transformation in wheat was achieved by ……..bombardment of callus from immature embryos. A wheat chloroplast site-specific expression vector pMY-Wt-nif, was constructed by placing an expression cassette containing neomycin phosphotransferase II (nptII) and nitrogen fixation gene cluster from Clostridium acetobutylicum in the intergenic spacer between trnI and trnA of wheat chloroplast genome. Integration of nitrogen fixation gene cluster in the plastome was identified by polymerase chain reaction (PCR) analysis and Southern blotting using specific sequence of the cassette as a probe. Expression of nitrogen fixation gene cluster was examined by Western blot. The results strengthen the feasibility of wheat chloroplast transformation and also give a novel method for the introduction of important agronomic traits in wheat through chloroplast transformation.

show abstract

Section: Discussionsupporting

confidence: 92%

Plastid Transformation of Wheat Cultivar Using Plastid Expression Cassette Carrying Nitrogen Fixation Genes

2016

Egypt. J. Bot.

View full text Add to dashboard Cite

show abstract

“…e use of spectinomycin-resistant aadA as a selectable marker markedly increased the transformation efficiency to 1-2.5 transformants per plate (Svab and Maliga 1993;Khan and Maliga 1999). Despite the difference in vector construction, such as homologous regions and selectable marker genes, plastid transformants were produced not only in tobacco (Daniell et al 1998;Bock et al 1996;Jeong et al 2004) but also in other species (Day and GoldschmidtClermont 2011;Cui et al 2011). However, plastid transformation e ciency in other species except tobacco was still very low, making it difficult to obtain one plastid transformant per bombarded plate.…”

mentioning

confidence: 99%

Improvement of the plastid transformation protocol by modifying tissue treatment at pre- and post-bombardment in tobacco

Okuzaki

Tabei

2012

Plant Biotechnology

View full text Add to dashboard Cite

Plastid transformation in higher plants has been established in tobacco using particle bombardment, and the protocol has been used as a model in other plant species; however, as target materials, tobacco leaves may be partially exposed to unexpected abiotic stresses-drought stress during post-bombardment culture and damage due to cutting before selection culture. Plastid transformation e ciency may be increased by modi cation of leaf treatment to lessen such damage. In the modi ed protocol, tobacco leaves were cut into pieces (5×5 mm) and placed on the culture medium plate (approximately y pieces per plate) for one day before bombardment. A er bombardment, these pieces were transferred onto the non-selection medium and cultured for three days; they were then transferred onto spectinomycin selection culture medium. A transformation vector containing an aminoglycoside 3′-adenylyltransferase gene and a green uorescence protein gene were used for plastid transformation. Approximately four independent plastid transformants per bombarded plate were obtained on average using the modi ed protocol; the transformation e ciency was 1.6 times higher than that in a control experiment using the standard protocol. Modi ed leaf treatment improved the e ciency and stability of plastid transformation. is nding should aid plastid transformant production in tobacco and other plant species.

show abstract

“…While this manuscript was in press, Cui and coworkers reported stable plastid transformation in wheat ( Triticum aestivum ) using the nptII selectable marker and G418 selection (Cui et al. , 2011).…”

Section: Note Added In Proofmentioning

confidence: 99%

The chloroplast transformation toolbox: selectable markers and marker removal

Day

Goldschmidt-Clermont

2011

Plant Biotechnology Journal

172

139

View full text Add to dashboard Cite

SummaryPlastid transformation is widely used in basic research and for biotechnological applications. Initially developed in Chlamydomonas and tobacco, it is now feasible in a broad range of species. Selection of transgenic lines where all copies of the polyploid plastid genome are transformed requires efficient markers. A number of traits have been used for selection such as photoautotrophy, resistance to antibiotics and tolerance to herbicides or to other metabolic inhibitors. Restoration of photosynthesis is an effective primary selection method in Chlamydomonas but can only serve as a screening tool in flowering plants. The most successful and widely used markers are derived from bacterial genes that inactivate antibiotics, such as aadA that confers resistance to spectinomycin and streptomycin. For many applications, the presence of a selectable marker that confers antibiotic resistance is not desirable. Efficient marker removal methods are a major attraction of the plastid engineering tool kit. They exploit the homologous recombination and segregation pathways acting on chloroplast genomes and are based on direct repeats, transient co-integration or co-transformation and segregation of trait and marker genes. Foreign site-specific recombinases and their target sites provide an alternative and effective method for removing marker genes from plastids.

show abstract

Stable chloroplast transformation of immature scutella and inflorescences in wheat (<italic>Triticum aestivum</italic> L.)

Cited by 30 publications

References 52 publications

Plastid Transformation of Wheat Cultivar Using Plastid Expression Cassette Carrying Nitrogen Fixation Genes

Plastid Transformation of Wheat Cultivar Using Plastid Expression Cassette Carrying Nitrogen Fixation Genes

Improvement of the plastid transformation protocol by modifying tissue treatment at pre- and post-bombardment in tobacco

The chloroplast transformation toolbox: selectable markers and marker removal

Contact Info

Product

Resources

About