Stable carbon isotopic composition of gasolines determined by isotope ratio monitoring gas chromatography mass spectrometry

Smallwood, Barbara J.; Philp, R. Paul; Allen, J.

doi:10.1016/s0146-6380(01)00142-5

Cited by 95 publications

(54 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As shown in Fig. 4a, the difference of ␦ 13 C rarely exceeded the total analytical uncertainty Ϯ 0.5‰ (47) and never left the typical range of ␦ 13 C values for MTBE in gasoline (Ϫ28.3‰ to Ϫ31.6‰) (22,48).…”

Section: Vol 77 2011 Ethb Gene Expression As Proof Of Mtbe Biodegramentioning

confidence: 88%

Linking Low-Level Stable Isotope Fractionation to Expression of the Cytochrome P450 Monooxygenase-Encoding ethB Gene for Elucidation of Methyl tert -Butyl Ether Biodegradation in Aerated Treatment Pond Systems

Jechalke

Rosell

Lavanchy

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

Multidimensional compound-specific stable isotope analysis (CSIA) was applied in combination with RNA-based molecular tools to characterize methyl tertiary (tert-) butyl ether (MTBE) degradation mechanisms occurring in biofilms in an aerated treatment pond used for remediation of MTBE-contaminated groundwater. The main pathway for MTBE oxidation was elucidated by linking the low-level stable isotope fractionation (mean carbon isotopic enrichment factor [ C ] of ؊0.37‰ ؎ 0.05‰ and no significant hydrogen isotopic enrichment factor [ H ]) observed in microcosm experiments to expression of the ethB gene encoding a cytochrome P450 monooxygenase able to catalyze the oxidation of MTBE in biofilm samples both from the microcosms and directly from the ponds. 16S rRNA-specific primers revealed the presence of a sequence 100% identical to that of Methylibium petroleiphilum PM1, a well-characterized MTBE degrader. However, neither expression of the mdpA genes encoding the alkane hydroxylase-like enzyme responsible for MTBE oxidation in this strain nor the related MTBE isotope fractionation pattern produced by PM1 could be detected, suggesting that this enzyme was not active in this system. Additionally, observed low inverse fractionation of carbon ( C of ؉0.11‰ ؎ 0.03‰) and low fractionation of hydrogen ( H of ؊5‰ ؎ 1‰) in laboratory experiments simulating MTBE stripping from an open surface water body suggest that the application of CSIA in field investigations to detect biodegradation may lead to false-negative results when volatilization effects coincide with the activity of low-fractionating enzymes. As shown in this study, complementary examination of expression of specific catabolic genes can be used as additional direct evidence for microbial degradation activity and may overcome this problem.

show abstract

Section: Vol 77 2011 Ethb Gene Expression As Proof Of Mtbe Biodegramentioning

confidence: 88%

Linking Low-Level Stable Isotope Fractionation to Expression of the Cytochrome P450 Monooxygenase-Encoding ethB Gene for Elucidation of Methyl tert -Butyl Ether Biodegradation in Aerated Treatment Pond Systems

Jechalke

Rosell

Lavanchy

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Stable carbon isotopic compositions of SOA precursors have been previously measured at their emission sources (Czapiewski et al, 2002;Rudolph et al, 2002;Rudolph, 2007;Smallwood et al, 2002) as well as in ambient air (Goldstein and Shaw, 2003;Kornilova, 2012;Rudolph, 2007). Recently, a measurement method for isotope ratios of methylnitrophenols in atmospheric particulate matter (PM) has been established (Moukhtar et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Method for the determination of concentration and stable carbon isotope ratios of atmospheric phenols

et al. 2013

View full text Add to dashboard Cite

Abstract.A method for the determination of the stable carbon isotopic composition of atmospheric nitrophenols in the gas and particulate phases is presented. It has been proposed to use the combination of concentration and isotope ratio measurements of precursor and product to test the applicability of results of laboratory studies to the atmosphere. Nitrophenols are suspected to be secondary products formed specifically from the photooxidation of volatile organic compounds. XAD-4 TM resin was used as an adsorbent on quartz filters to sample ambient phenols using conventional high volume air samplers at York University in Toronto, Canada. Filters were extracted in acetonitrile, with a HPLC (high-performance liquid chromatography) clean-up step and a solid phase extraction step prior to derivatization with BSTFA (bis(trimethylsilyl) trifluoroacetamide). Concentration measurements were done with gas chromatographymass spectrometry and gas chromatography-isotope ratio mass spectrometry was used for isotope ratio analysis.The technique presented allows for atmospheric compound-specific isotopic composition measurements for five semi-volatile phenols with an estimated accuracy of 0.3-0.5 ‰ at atmospheric concentrations exceeding 0.1 ng m −3 while the detection limits for concentration measurements are in the pg m −3 range. Isotopic fractionation throughout the entire extraction procedure and analysis was proven to be below the precision of the isotope ratio measurements. The method was tested by conducting ambient measurements from September to December 2011.

show abstract

“…In addition, water washing and biodegradation will lead to changes in the distribution of the volatile compounds in a groundwater/gasoline plume compared to that of the sample in the original tank [310]. Therefore, isotopic fingerprints of gasolines from different sources are an alternative or complimentary tool to be used with more conventional GC analyses [313,314].…”

Section: Forensic Researchmentioning

confidence: 99%

Gas chromatography coupled to mass spectrometry for analyses of organic compounds and biomarkers as tracers for geological, environmental, and forensic research

Medeiros

Simoneit

2007

J of Separation Science

View full text Add to dashboard Cite

ReviewGas chromatography coupled to mass spectrometry for analyses of organic compounds and biomarkers as tracers for geological, environmental, and forensic research Gas chromatography, especially when coupled with mass spectrometry, is the analytical method of choice for elucidation of biomarker compounds present in organic mixtures extracted from geological, environmental, and biological samples. This review describes the biomarker concept, i. e., the precursor natural products to the geological/environmental derivatives, and their application as multi-tracers in the geosphere and ambient environment. The mass spectrometric methods currently utilized for such analyses are reviewed with a general key to the literature, and typical examples of applications using GC -MS are also described.

show abstract

Stable carbon isotopic composition of gasolines determined by isotope ratio monitoring gas chromatography mass spectrometry

Cited by 95 publications

References 12 publications

Linking Low-Level Stable Isotope Fractionation to Expression of the Cytochrome P450 Monooxygenase-Encoding ethB Gene for Elucidation of Methyl tert -Butyl Ether Biodegradation in Aerated Treatment Pond Systems

Linking Low-Level Stable Isotope Fractionation to Expression of the Cytochrome P450 Monooxygenase-Encoding ethB Gene for Elucidation of Methyl tert -Butyl Ether Biodegradation in Aerated Treatment Pond Systems

Method for the determination of concentration and stable carbon isotope ratios of atmospheric phenols

Gas chromatography coupled to mass spectrometry for analyses of organic compounds and biomarkers as tracers for geological, environmental, and forensic research

Contact Info

Product

Resources

About