Stable antibacterial silver nanoparticles produced with seed-derived callus extract of Catharanthus roseus

Abstract

Biocompatibility and ecotoxicity concerns associated with chemically produced metallic nanoparticles have led to an increasing interest in the development of environmentally benign alternatives for nanoparticle synthesis using biological platforms. Herein, we report the utilization of an extract of seed-derived callus of Catharanthus roseus for the production of stable silver nanoparticles (Ag NPs). The bioreduction of silver ions was evident from UV-Vis spectroscopy results: the absorption maxima were observed at 425 nm, indicative of elemental silver. Transmission electron micrographs revealed that the Ag NPs were well-dispersed and predominantly spherical with particle sizes in the range of 2-15 nm. The synthesized Ag NPs exhibited colloidal stability in an aqueous dispersion for a period of 120 days, as indicated by UV-Vis absorbance spectra and zeta potential measurements. Fourier transform infrared spectroscopy revealed the possible utilization of hydroxyl groups and amides in the reduction of silver ions and surface stabilization of the Ag NPs, respectively. Notably, the synthesized Ag NPs showed considerable antibacterial action against Escherichia coli even after 8 weeks of storage under ambient conditions. Thus, cell extracts of cultured callus of Catharanthus roseus could be explored as an ecofriendly platform for the synthesis of stable and functional nanoparticles.

Augmented photocatalytic, antibacterial and antifungal activity of prunosynthetic silver nanoparticles

“…ultraviolet irradiation, aerosol assisted techniques, photochemical routes, etc. The exorbitant nature and utilization of environmentally perilous raw materials and requirement for elevated temperatures make these techniques a serious presage to biota [19]. However, the prunosynthesis of AgNPs serves as an effective and sustainable alternate for it is carried out with abundantly Prunus cerasifera fruits through ecologically benign pathway.…”

Safed Musli (Chlorophytum borivilianum L.) Callus-Mediated Biosynthesis of Silver Nanoparticles and Evaluation of their Antimicrobial Activity and Cytotoxicity against Human Colon Cancer Cells

“…A CO 2 incubator adjusted to 37°C was used to incubate the cells for 48 hours. To study the cytotoxicity, the cells were treated with biosynthesized AgNPs (10,20,40,80,120, and 160 μg/mL) and incubated for 48 hours to evaluate cell survivability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. Firstly, fresh MTT solution (5 mg/mL) was prepared and about 10 mL of it was dispensed to each well.…”

“…Recently, AgNPs are being synthesized from the plant callus as a novel source. For instance, the callus induced from Catharanthus roseus, Sesuvium portulacastrum, Taxus yunnanensis, Centella asiatica, Cucurbita maxima, etc., are used for the biosynthesis of AgNPs [4,[18][19][20][21]. Advantageously, callus cultures mitigate the problems of wild plant source scarcity.…”

Osseointegration of hydroxyapatite coatings doped with silver nanoparticles: scanning electron microscopy studies on a rabbit model

“…Electrochemically formed silver coatings were one of early solutions used in resection prostheses since early 2000s [11,21]. In conventional uncemented components such coatings may be problematic, due to concerns regarding osseointegration and silver toxicity; however, silver nanoparticles (SNs) have recently emerged as an alternative [14,21]. They can be distributed on the surface of uncemented components without compromising the osseointegrative layer [6].…”