Stabilizing to disruptive transition of focal adhesion response to mechanical forces

Kong, Dong; Ji, Baohua; Dai, L.H.

doi:10.1016/j.jbiomech.2010.05.019

Cited by 35 publications

(31 citation statements)

References 47 publications

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“…1a and 1b. Previous study 38 showed that the responses of FAs to the stretching have the ''stabilizing to disruptive'' transition as the applied force increases. This observation suggested that there is an optimum region of the force value in which the FAs are stable.…”

Section: Stress Fibermentioning

confidence: 97%

“…(9) and Fig. 2 was given by previous studies 38 that there were two force limits which set three regions of force for different states of stability of FAs. When the force is lower than the lower limit, the integrins can not be activated and therefore the FAs are small and unstable; on the other hand, when the force is higher than the upper limit, FAs will disassemble because of bond rupture.…”

Section: Stress Fibermentioning

confidence: 97%

“…These kinds of dynamics could be caused by the changing of either the internal contractile force of cells or the internal and external chemical signals. However, in the stretching experiments we modeled in this study, the force applied on the cell could be much larger than the contractile force or the effect of chemical signals so that the stretching force could even cause the disassembly of FAs and then the cell reorientation (see previous works 36,38 ), particularly at high loading frequency. Therefore, in this work, we assumed that the effect of these changing of the contractile force and chemical signals were so small compared with the external stretching force that they could be neglected.…”

Section: Stress Fibermentioning

confidence: 99%

“…Recently, Kong et al 38 proved that the force for inducing the rupture of FA is much larger than that for inducing FA's growth. Here we focused on the frequency dependent instability of mature FA under substrate stretching without explicitly considering clustering of integrin-ligand bonds for simplicity.…”

Section: A Microscopic Bond-cluster Model Of Famentioning

confidence: 99%

“…However, if the force deviates from this optimum region, the FAs will start to disassemble. The probability of the disassembling of FAs can be described by an empirical relation according to previous studies 28,38 as…”

Section: Stress Fibermentioning

confidence: 99%

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Frequency-Dependent Focal Adhesion Instability and Cell Reorientation Under Cyclic Substrate Stretching

et al. 2011

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Associate Editors Mian Long and Fan Yuan oversaw the review of this article.Abstract-The adhesion-based cell mechanosensitivity plays central roles in many physiological and pathological processes. Recently, quantitative understanding of cell responses to external force has been intensively pursued. However, the frequency dependent cell responses to the substrate stretching have not yet been fully understood. Here we developed a multiscale modeling framework for studying cell reorientation behaviors under substrate stretching, in which the mechano-chemical coupling at molecular, subcellular, and cellular scales was considered. The effect of matrix stiffness was also considered in a FEM based mechano-chemical coupling simulation. We showed that the collapsing time of focal adhesion decreases with the increasing of the loading frequency, however, the cell reorientation time exhibits a biphasic frequency-dependent behavior. Our results suggested that this biphasic behavior might be caused by the competition between the frequency-dependent collapsing of focal adhesions and the less frequency-dependent formation of stress fibers aligning away from the loading direction. At the low loading frequency, the collapsing of focal adhesion dominates the reorientation process, however, at the high loading frequency the polymerization of stress fiber dominates the reorientation. Moreover, we showed that the compliance of matrix may help accelerate the cell reorientation because focal adhesion is prone to be instable on soft matrix.

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Section: Stress Fibermentioning

confidence: 97%

Section: Stress Fibermentioning

confidence: 97%

Section: Stress Fibermentioning

confidence: 99%

Section: A Microscopic Bond-cluster Model Of Famentioning

confidence: 99%

Section: Stress Fibermentioning

confidence: 99%

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Frequency-Dependent Focal Adhesion Instability and Cell Reorientation Under Cyclic Substrate Stretching

et al. 2011

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Substrates with Patterned Extracellular Matrix and Subcellular Stiffness Gradients Reveal Local Biomechanical Responses

Tseng

Carlo

2013

Advanced Materials

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A substrate fabrication process is developed to pattern both the extracellular matrix (ECM) and rigidity at sub‐cellular spatial resolution. When growing cells on these substrates, it is found that cells respond locally in their cytoskeleton assembly. The presented method allows unique insight into the biological interpretation of mechanical signals, whereas photolithography‐based fabrication is amenable to integration with complex microfabricated substructures.

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Review and perspective on soft matter modeling in cellular mechanobiology: cell contact, adhesion, mechanosensing, and motility

Zhang

Feng

2017

Acta Mech

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Stabilizing to disruptive transition of focal adhesion response to mechanical forces

Cited by 35 publications

References 47 publications

Frequency-Dependent Focal Adhesion Instability and Cell Reorientation Under Cyclic Substrate Stretching

Frequency-Dependent Focal Adhesion Instability and Cell Reorientation Under Cyclic Substrate Stretching

Substrates with Patterned Extracellular Matrix and Subcellular Stiffness Gradients Reveal Local Biomechanical Responses

Review and perspective on soft matter modeling in cellular mechanobiology: cell contact, adhesion, mechanosensing, and motility

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