AbstrakBuah atung (Parinarium glaberimmum Hassk) adalah tumbuhan hutan tropis yang berpotensi sebagai sumber bahan antimikroba yang banyak tumbuh di Kawasan Timur Indonesia terutama di daerah Maluku. Penelitian ini bertujuan untuk menentukan karakteristik kimia pada ikan tongkol asap setelah direndam pada ekstrak biji atung. Pengukuran parameter kimia meliputi kadar air, kadar abu, kadar lemak, kadar protein metode Mikro Abu Kjeldahl (AOAC 2005), karbohidrat (metode by difference) dan mikrobiologi (ALT). Pengamatan dilakukan setiap 2 hari selama 4 hari penyimpanan. Rancangan yang digunakan adalah Rancangan Acak Lengkap Faktorial dengan 3 ulangan. Data dianalisa statistic univariate menggunakan SPSS 20. Hasil penelitian menunjukkan bahwa ikan tongkol asap yang diberi perlakuan perendaman dalam ekstrak biji atung berpengaruh nyata (α=5%) terhadap karakteristik kimia dan jumlah total mikroba selama peniympanan suhu ruang. Perlakuan perendaman ikan asap dalam larutan atung merupakan perlakuan terbaik di awal (0 hari) pengasapan dengan jumlah total mikroba 2,6x10 4 koloni. Perlakuan terbaik berdasarkan parameter kimia diperoleh pada perlakuan perendaman ikan tongkol dalam ekstrak atung selama 2 hari penyimpanan dalam suhu ruang. Nilai karaktersitik kimia terbaik ikan tongkol asap yaitu kadar air 59,46%, kadar abu 2,66%, kadar lemak 1,63%, kadar protein 34,62% dan karbohidrat 1,63%.Kata kunci: Biji atung, mutu, pengawet alami Abstract Atung (Parinarium glaberimmum Hassk) was tropical plant as potential as antimicrobial much grown in Eastern Indonesia especially in Maluccas area. The aim of this research was to determine the chemical characteristics of smoked swordfish was soak in extract atung seed before smoking. Measurement parameter of chemical characteristics covering the moisture conten, levels of ash, levels of fat, levels of a protein micro method Kjeldahl ash (AOAC 2005), carbohydrates (method by difference) and microbiologi. The observation is made every day storage 2 days up 4 days. This research used Factorial Randomized Design with 3 replication. Data analysis statistic univariate using software SPSS 20. The results of this research show the swordfish were soak in extract atung seed before smoking affect the chemical characteristics and microbiologi. Swordfish smoked with extract atung seed and room tempareture storage were given significant effect (α=5%) to the microbes total. The best value of swordfish in soak of atung extract at first production with value of microbes 2,6x10 4 CFU. The chemical characteristics analysis result show differences among the treatments during the storage room temperature. The best values of chemical characteristics swordfish smoked at 2 days room temperature storage with moisture level 59,46%, ash level 2,66%, fat level 1,63%, protein level 34,62% and carbohydrat level 1,63%.