Stabilized Normal-Phase High-Performance Liquid Chromatographic Analysis of Aspirin and Salicylic Acid in Solid Pharmaceutical Dosage Forms

A fast ultra high pressure liquid chromatographic method for qualification and quantification of pharmaceutical combination preparations containing paracetamol, acetyl salicylic acid and/or antihistaminics

“…Both normal phase [3] as reversed phase [4][5][6][7][8][9][10][11][12][13] methods can be found. Further methods can be found using LC-MS [14,15], capillary electrophoresis [16][17][18][19][20][21] and micellar electrokinetic chromatography [17,20,22].…”

Determination of Aspirin by Pre-Column Transacetylation Reaction of 3-Aminophenol and Reversed-Phase High-Performance Liquid Chromatography: Simultaneous Determination of Aspirin, Acetaminophen, and Caffeine

“…extrapolation of results back to time zero,l0 and selection of solvents to minimize salicylic acid formati0n.~. 9,15 The aim of the present work is to make use of t h e chemical reactivity of the acetyl group of aspirin as a pre-column reaction in a direct approach to its determination. It has been found that aspirin quickly transacetylates 3-aminophenol producing 3-hydroxyacetanilide, which is a stable compound and can be determined by reversed-phase HPLC without any interference from salicylic acid and other ingredients of drug formulation.…”

Spectrophotometric determination of aspirin by trans acetylation of 4-aminophenol

“…Conversion of aspirin into salicylic acid during sample preparation is a vexing problem and it can limit the accuracy of determination. Attempts to obviate or minimize errors from this source include injection of sample within a few minutes of its preparation (21), extrapolation of results back to time zero (5), preparation of calibrated standards with matching amounts of aspirin (7), selection of solvents to minimize salicylic acid formation (4,20,22), and separation of aspirin from product ingredients that accelerate its degradation (22). Complex formulations have been analyzed by ultraviolet absorption after separation of their constituents by partition chromatography (23)(24)(25).…”