Stabilization of the tertiary structure of yeast phenylalanine tRNA by [Co(NH3)6]3+

Hingerty, Brian E.; Brown, Raymond S.; Klug, A.

doi:10.1016/0167-4781(82)90047-1

Cited by 44 publications

(30 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the case of Co(NH3)63+, Gessner et al (32) showed that the interaction of this cation with (dC-dG)3 is stabilized by five hydrogen bonds formed between the ammonia molecules of Co(NH3)63+ and the phosphate groups of the oligonucleotide as well as the N7 and 06 positions on guanine. A similar hydrogen bonding pattern has been observed in Co(NH3)63+-induced stabilization of the tertiary structure of yeast phenylalanine tRNA (33).…”

Section: Discussonsupporting

confidence: 52%

Direct evidence for the presence of left-handed conformation in a supramolecular assembly of polynucleotides

Thomas¹

1989

Nucl Acids Res

View full text Add to dashboard Cite

Hexammine cobalt(lIl) chloride (Co(NH3)63+) provokes a B-DNA -4 Z-DNA -+ P-DNA conformational transition in poly(dG-dC).poly(dG-dC) and poly(dG-m5dC).poly(dG-m5dC). The circular dichroism spectrum of '-DNA is characterized by a manyfold increase of positive ellipticity in the range of 300-225 nm and the complete absence of a negative peak. In order to ascertain the helical handedness of '-DNA, we used a recently developed enzyme immunoassay technique. This method consisted of treating the polynucleotides with Co(NH3)63+ to convert them to the Z-or '-DNA forms and immobilizing these conformations on a microtiter plate.The plates were subsequently treated with a monoclonal anti-Z-DNA antibody Z22, alkaline phosphatase conjugated, affinity purified immunoglobulins, and the phosphatase substrate. The enzyme-substrate reaction was monitored by reading the absorbance at 405 nm with a microplate autoreader. The monoclonal anti-Z-DNA antibody had no reactivity to the B-DNA form, but bound strongly to both the Z-and '-DNA forms, showing that Co(NH3)63+-induced '-DNA form of the polynucleotides exists in the left-handed Z-DNA conformation. INTRODQUCTIONMultivalent ions like Co(NH3)63+ and Ru(NH3)63+ are excellent promoters of the transition of poly(dG-dC).poly(dG-dC) and poly(dG-m5dC).poly(dG-m5dC) from their usual right-handed B-DNA form to the left-handed Z-DNA conformation at micromolar concentrations of these counterions (1-4). Increasing concentrations of Co(NH3)63+ are also known to provoke a supramolecular organization of the polynucleotides from the Z-DNA form to a '-DNA form (5-6). The '-DNA form is a believed to be a twisted, tightly packaged assembly of DNA.Several agents, including polylysine, spermine, ethylene glycol, and salt are known to convert native DNAs to the '-DNA form (7-9). The formation of this structural form of DNA is characterized by a large (10-to 100-fold) increase in the intensity of the molar ellipticity of DNA in circular dichroism (CD) spectroscopic measurements. This property has presented a basic problem in interpreting the CD spectrum of '-DNA and assigning its helical handedness. This uncertainty has recently led to the conclusion that '-DNA is the end product in the

show abstract

Section: Discussonsupporting

confidence: 52%

Direct evidence for the presence of left-handed conformation in a supramolecular assembly of polynucleotides

Thomas¹

1989

Nucl Acids Res

View full text Add to dashboard Cite

show abstract

“…Evidence is accumulating that Co(NH3)v and spermidine bind to polynucleotide double helices in rather different ways. Hingerty et al 27 have recently determined the binding of Co(NH3):' to yeast phenylalanyl tRNA by x-ray diffraction. Three such ions bind, all to purine-purine sequences.…”

Section: Light-scattering Apparatus and Data Analysismentioning

confidence: 98%

Collapse of DNA caused by trivalent cations: pH and ionic specificity effects

Thomas¹,

Bloomfield²

1983

Biopolymers

View full text Add to dashboard Cite

SynopsisA comparison of the condensation of T4 phage DNA by spermidine and Co(NH&+ at pH values between 5.1 and 10.2 has been made using quasielastic light scattering to determine translational diffusion coefficients and Stokes radii. Co(NH&' is more effective than spermidine in causing condensation at all pH, indicating that the differences observed in previous work were not due to pH effects, as might have been inferred from recent theories of intermolecular forces. The DNA particles collapsed with Co(NH& are smaller than those obtained with spermidine. The hydrodynamic radius of the spermidine-collapsed structures decreases slightly with increasing pH, while the size of the Co(NH&+ collapsed structures is almost independent of pH. These results confirm that there are specific ion effects in DNA condensation by oligocations, in addition to the dominant general polyelectrolyte effects.

show abstract

“…Recent structural analyses of RNA and DNA and their metal-adducts reveal the presence of RNA in A-form (12), while DNA exists in A-, B-, and Z-forms (4,9,19). On the other hand, X-ray structural data showed (20) that mononucleotides, such as, 5' -GMPNa 2 and 5'-IMPNa 2 have the C/-endo conformation, whereas upon coordination to metal ions at the N 7 -site of the imidazol ring or at the phosphate group, the C 3 '-endo conformation becomes predominent.…”

Section: Rna and Dna Conformational Transitionsmentioning

confidence: 99%

“…1 and Table 1). It is worth mentioning that the crystal structures of tRNA complexes of [Co(NH 3 ) 6 ]Cl 3 (12) and [Ru(NH 3 )sCl] Cl 2 and cis-platinum have been reported (23). The cobalt hexammine binds to the N 7 -site and 0 6 oxygen atom of the two adjacent guanine bases via two hydrogen bonded ammine groups, while ruthenium pentammine and cis-platinum bind directly to the N 7 -site of the guanine residue (Pt-N 7 ) and hydrogen bonded to the 0 6 and the phosphate group through the ammine groups.…”

Section: Rna-metal Complexesmentioning

confidence: 99%

Flexibility of DNA and RNA upon Binding to Different Metal Cations. An Investigation of the B to A to Z Conformational Transition by Fourier Transform Infrared Spectroscopy

Théophanides

Tajmir-Riahi

1985

Journal of Biomolecular Structure and Dynamics

View full text Add to dashboard Cite

The interaction of DNA and RNA with Cu(II), Mg(II), [Co(NH3)6]3+ [Co(NH3)5Cl]2+ chlorides and, cis- and trans-Pt(NH3)2Cl2 (CIS-DDP, trans-DDP) has been studied by Fourier Transform Infrared (FT-IR) spectroscopy and a correlation between metal-base binding and conformational transitions in the sugar pucker has been established. It has been found that RNA did not change from A-form on complexation with metals, whereas DNA exhibited a B to Z transition. The marker bands for the A-form (C3'-endo-anti conformation) were found to be near 810-816 cm-1, while the bands at 825 and 690 cm-1 are marker bands for the B-conformation (C2'-endo, anti). The B to Z (C3'-endo. syn conformation) transition is characterized by the shift of the band at 825 cm-1 to 810-816 cm-1 and the shift of the guanine band at 690 cm-1 to about 600-624 cm-1.

show abstract

Stabilization of the tertiary structure of yeast phenylalanine tRNA by [Co(NH3)6]3+

Cited by 44 publications

References 13 publications

Direct evidence for the presence of left-handed conformation in a supramolecular assembly of polynucleotides

Direct evidence for the presence of left-handed conformation in a supramolecular assembly of polynucleotides

Collapse of DNA caused by trivalent cations: pH and ionic specificity effects

Flexibility of DNA and RNA upon Binding to Different Metal Cations. An Investigation of the B to A to Z Conformational Transition by Fourier Transform Infrared Spectroscopy

Contact Info

Product

Resources

About