Stabilization of the phosphorylated form of<i>Bacillus subtilis</i>DegU caused by<i>degU9</i>mutation

Takano, Teruo; Kawata-Mukai, M

doi:10.1111/j.1574-6968.1994.tb06620.x

Cited by 4 publications

References 10 publications

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A search for amino acid substitutions that universally activate response regulators

Smith

Latiolais

Guanga

et al. 2003

Molecular Microbiology

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SummaryTwo-component regulatory systems, typically composed of a sensor kinase to detect a stimulus and a response regulator to execute a response, are widely used by microorganisms for signal transduction. Response regulators exhibit a high degree of structural similarity and undergo analogous activating conformational changes upon phosphorylation. The activity of particular response regulators can be increased by specific amino acid substitutions, which either prolong the lifetime or mimic key features of the phosphorylated state. We probed the universality of response regulator activation by amino acid substitution. Thirty-six mutations that activate 11 different response regulators were identified from the literature. To determine whether the activated phenotypes would be retained in the context of a different response regulator, we recreated 51 analogous amino acid substitutions at corresponding positions of CheY. About 55% of the tested substitutions completely or partially inactivated CheY, ª ª ª ª 30% were phenotypically silent, and ª ª ª ª 15% activated CheY. Three previously uncharacterized activated CheY mutants were found. The 94NS (and presumably 94NT) substitutions resulted in resistance to CheZ-mediated dephosphorylation. The 113AP substitution led to enhanced autophosphorylation and may increase the fraction of non-phosphorylated CheY molecules that populate the activated conformation. The locations of activating substitutions on the response regulator three-dimensional structure are generally consistent with current understanding of the activation mechanism. The best candidates for potentially universal activating substitutions of response regulators identified in this study were 13DK and 113AP.

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A search for amino acid substitutions that universally activate response regulators

Smith

Latiolais

Guanga

et al. 2003

Molecular Microbiology

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A Signal Transduction Network inBacillus subtilisIncludes the DegS/DegU and ComP/ComA Two-Component Systems

Msadek

Kunst

Rapoport

2014

Two-Component Signal Transduction

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Multiple copies of the proB gene enhance degS-dependent extracellular protease production in Bacillus subtilis

et al. 1994

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Bacillus subtilis secretes extracellular proteases whose production is positively regulated by a two-component regulatory system, DegS-DegU, and other regulatory factors including DegR. To identify an additional regulatory gene(s) for exoprotease production, we performed a shotgun cloning in the cell carrying multiple copies of degR and found a transformant producing large amounts of the exoproteases. The plasmid in this transformant, pLC1, showed a synergistic effect with multiple copies of degR on the production of the extracellular proteases, and it required degS for its enhancing effect. The DNA region responsible for the enhancement contained the proB gene, as shown by restriction analyses and sequence determination. The proB gene encoding Sy-glutamyl kinase was followed by the proA gene encoding glutamyl--y-semialdehyde dehydrogenase at an interval of 39 nucleotides, suggesting that the genes constitute an operon. pLC1 contained the complete proB gene and a part ofproA lacking the proA C-terminal region. It was also found that proB on the chromosome showed a synergistic effect with multiple copies of degR. We consider on the basis of these results that the metabolic intermediate, y-glutamyl phosphate, would transmit a signal to DegS, resulting in a higher level of phosphorylated DegU. Possible involvement of DegR in this process is discussed.

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Stabilization of the phosphorylated form ofBacillus subtilisDegU caused bydegU9mutation

Cited by 4 publications

References 10 publications

A search for amino acid substitutions that universally activate response regulators

A search for amino acid substitutions that universally activate response regulators

A Signal Transduction Network inBacillus subtilisIncludes the DegS/DegU and ComP/ComA Two-Component Systems

Multiple copies of the proB gene enhance degS-dependent extracellular protease production in Bacillus subtilis

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