Stabilization of the glucan-binding lectin of Streptococcus sobrinus by specific ligand

Denson, Andrea M; Doyle, Ronald J.

doi:10.1016/s0003-9969(97)00089-7

Cited by 3 publications

(2 citation statements)

References 28 publications

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“…1988), and with other proteins for glucose residues in the acquired pellicle dextrans. This suggestion is supported by the observation that S. sobrinus synthesizes several proteins that could bind to the commercial polidextran Sephadex G‐75 (Denson and Doyle 1998), constituted by a cross‐linked dextran which is also a component of Sephadex G‐50, used as affinity column to purify the glucose–mannose lectins assayed in this article. Although the involvement of a galactose‐specific lectin has been described in S. mutans adhesion process (Gibbons and Quereshi 1979), the sole galactose‐specific lectin assayed, VML was able to inhibit only the adhesion of S. sanguis.…”

Section: Discussionsupporting

confidence: 60%

In vitro inhibition of Streptococci binding to enamel acquired pellicle by Plant Lectins

et al. 2006

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Aim: Initial colonization of the tooth surface by streptococci involves the attachment of these bacteria to adsorbed salivary components of the acquired pellicle. In dental biofilm this adhesion may also involve lectin‐like components, present on the surface of the organisms, which bind to complementary carbohydrates on the surface of the tooth. Therefore, this work aimed to evaluate the potential of six lectins, extracted from seeds of Leguminosae family members, to inhibit the adherence of five streptococci species to acquired pellicle in vitro. Methods and Results: The lectins used in this work were extracted from Canavalia ensiformis, Canavalia brasiliensis, Dioclea violacea, Dioclea grandiflora, Cratylia floribunda and Vatairea macrocarpa. Fluorescence micrography was employed to visualize the ability of FITC‐labeled lectins to attach to acquire pellicle. Adherence inhibition was performed on saliva‐coated microtiter plates at which lectins solutions were previously incubated followed by incubation with the oral streptococci. Glucose‐mannose specific lectins attached to acquired pellicle with high intensity, while galactose specific lectins, from V. macrocarpa, exhibits low intensity attachment. Conclusions: All lectins were able to inhibit the adherence of the microorganisms tested (p < 0·01). Significance and Impact of the Study: Our results suggest that lectins may be useful in anti adhesion therapeutics.

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Section: Discussionsupporting

confidence: 60%

In vitro inhibition of Streptococci binding to enamel acquired pellicle by Plant Lectins

et al. 2006

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“…S. mutans can synthesize firmly-bound glucan from sucrose by the cooperative actions of glucosyltransferase (GTF) (Hamada et al, 1984). In addition, glucan-binding lectin (GBL), produced by the bacteria, plays an important role in glucan-dependent cell aggregation (Denson and Doyle, 1998). A promising approach for the prophylaxis of dental caries is then based on the inhibition of the S. mutans adherence to hydroxyapatite and subsequent colonization of the tooth surface (Tarsi et al, 1997).…”

Section: Introductionmentioning

confidence: 99%