Stabilization of tetanus toxoid formulation containing aluminium hydroxide adjuvant against agitation

Nucleic Acid Aptamers Stabilize Proteins Against Different Types of Stress Conditions

“…). These values are similar to what has been reported earlier . In the presence of aptamers, the stability of the adsorbed toxoid against agitation increased significantly.…”

“…Trehalose, being a cryoprotectant, was able to retain the antigenicity, although more amount of protein was desorbed from the matrix in this case. When the same preparation was subjected to agitation, trehalose was not able to stabilize the protein, as seen in the case earlier with the monoclonal antibody . The best results for retention of antigenicity of the toxoid subjected to agitation were obtained with glucose and sorbitol.…”

“…Thus, these stabilizers are condition specific, that is, they are able to stabilize the protein against one kind of stress condition but may fail to stabilize it against another stress. This is because a stress condition such as heat may affect the solvent and thus the osmolyte–solvent interaction, in a different manner than another kind of stress, such as freezing or agitation . Osmolytes may prove to be better stabilizers against a particular set of stress condition.…”

“…Dialyzed tetanus toxoid (3.2 mL, 6.2 mg/mL) was incubated with alumina (5 mL, 13 mg/mL) overnight with mild shaking at 4°C, as described earlier . The amount of protein in the supernatant was estimated using dye binding method after the matrix settled down.…”

“…The best results for retention of antigenicity of the toxoid subjected to agitation were obtained with glucose and sorbitol. Depending on the stress the protein is exposed, to the requirement of the osmolyte changes . Thus, there is a need to search for novel stabilizers that will be able to stabilize proteins against a wider variety of stress conditions.…”