1983
DOI: 10.1073/pnas.80.7.2059
|View full text |Cite
|
Sign up to set email alerts
|

Stabilization of proteins by a bacteriophage T4 gene cloned in Escherichia coli.

Abstract: The cloned bacteriophage T4 pin gene functions to stabilize several different kinds of proteins in Escherichia coli bacteria. Incomplete proteins such as puromycyl polypeptides, abnormal but complete proteins such as the A phage tsO protein, and labile eukaryotic proteins encoded by genes cloned in E. coli such as mature human fibroblast interferon are stabilized in cells in which the T4 pin gene is expressed. The cloned T4 pin gene does not seem to affect the turnover of normal E. coli proteins.The introducti… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

2
10
0
1

Year Published

1987
1987
2008
2008

Publication Types

Select...
4
4

Relationship

1
7

Authors

Journals

citations
Cited by 33 publications
(13 citation statements)
references
References 24 publications
2
10
0
1
Order By: Relevance
“…The effects of pin on various proteins that are usually unstable in E. coli were examined. A marked decrease in the degradation of puromycyl polypeptides was observed in cells in which T4 pin was expressed (38); these results were similar to earlier results with the complete phage (39). In addition, pin increased the stability of cloned mature, human fibroblast interferon in E. coli (38).…”
supporting
confidence: 79%
See 2 more Smart Citations
“…The effects of pin on various proteins that are usually unstable in E. coli were examined. A marked decrease in the degradation of puromycyl polypeptides was observed in cells in which T4 pin was expressed (38); these results were similar to earlier results with the complete phage (39). In addition, pin increased the stability of cloned mature, human fibroblast interferon in E. coli (38).…”
supporting
confidence: 79%
“…A marked decrease in the degradation of puromycyl polypeptides was observed in cells in which T4 pin was expressed (38); these results were similar to earlier results with the complete phage (39). In addition, pin increased the stability of cloned mature, human fibroblast interferon in E. coli (38). The 6.0 x 104 to 6.0 x 106 ml-' and incubated for 2 h at 30°C with aeration to permit phage multiplication.…”
supporting
confidence: 77%
See 1 more Smart Citation
“…The two sets of plasmids constructed were transformed into E. coli S17-1(pir) (Simon et al 1983) by electroporation and transformants selected on MHA supplemented with Ap (100 µg mL) and Tc (20 µg/mL). Single colonies were purified and screened by PCR.…”
Section: Construction Of Stm Mutant Libraries Of Strain Lesb58mentioning
confidence: 99%
“…Inhibition of protein degradation requires synthesis of T4 proteins made during the first 10 min after infection at 37°C (18 -20). Clones of T4 genes that lead to inhibition of proteolysis in E. coli cells were obtained by Simon and co-workers (19,20), and one specific gene, pinA (proteolysis inhibition A), which resulted in inhibition of abnormal protein degradation, was cloned by Skorupski et al (21). The target of PinA in vivo appears to be the ATP-dependent Lon protease, because E. coli lon ϩ cells expressing a single copy of the T4 pinA gene are phenotypically Lon Ϫ (21).…”
mentioning
confidence: 99%