Stabilization of Indocyanine Green by Encapsulation within Micellar Systems

Kirchherr, Anne-Katrin; Briel, Andreas; Mäder, Karsten

doi:10.1021/mp8001649

Cited by 235 publications

(233 citation statements)

References 59 publications

Supporting

Mentioning

223

Contrasting

Order By: Relevance

“…It indicated that the liposomes could reduce the photobleaching of Cypate and maintain a stable structure in aqueous solution at acidic and physiological pH, leading to the prevention of Cypate from the degradation in acidic endocytic compartments at tumor due to its efficient encapsulation in the liposomes. 35 As shown in Figure 2C, the average diameters of Cypate/DOX-BTSL and Cypate/DOX-L remain around the initial sizes of liposomes without aggregation and precipitation in 10 d, suggesting a great stability of Cypate/ DOX-BTSL and Cypate/DOX-L.…”

Section: Results and Discussion Preparation And Characterization Of Lmentioning

confidence: 73%

NIR responsive liposomal system for rapid release of drugs in cancer therapy

Chen¹,

Liu²,

Su³

et al. 2017

IJN

View full text Add to dashboard Cite

To design a rapid release liposomal system for cancer therapy, a NIR responsive bubble-generating thermosensitive liposome (BTSL) system combined with photothermal agent (Cypate), doxorubicin (DOX), and NH 4 HCO 3 was developed. Cypate/DOX-BTSL exhibited a good aqueous stability, photostability, and photothermal effect. In vitro release suggested that the amounts of DOX released from BTSL were obviously higher than that of (NH 4 ) 2 SO 4 liposomes at 42°C. After NIR irradiation, the hyperthermic temperature induced by Cypate led to the decomposition of NH 4 HCO 3 and the generation of a large number of CO 2 bubbles, triggering a rapid release of drugs. Confocal laser scanning microscope and acridine orange staining indicated that Cypate/DOX-BTSL upon irradiation could facilitate to disrupt the lysosomal membranes and realize endolysosomal escape into cytosol, improving the intracellular uptake of DOX clearly. MTT and trypan blue staining implied that the cell damage of Cypate/DOX-BTSL with NIR irradiation was more severe than that in the groups without irradiation. In vivo results indicated that Cypate/DOX-BTSL with irradiation could dramatically increase the accumulation of DOX in tumor, inhibit tumor growth, and reduce systemic side effects of DOX. These data demonstrated that Cypate/DOX-BTSL has the potential to be used as a NIR responsive liposomal system for a rapid release of drugs in thermochemotherapy.

show abstract

Section: Results and Discussion Preparation And Characterization Of Lmentioning

confidence: 73%

NIR responsive liposomal system for rapid release of drugs in cancer therapy

Chen¹,

Liu²,

Su³

et al. 2017

IJN

View full text Add to dashboard Cite

show abstract

“…Kolliphor HS 15 was selected based on its outstanding in vivo safety and previously demonstrated effectiveness for imaging contrast agent formulas such as ICG. 22,[25][26] 500 μl batches of this medium were directly pipetted into the microtubes containing the dye at RT. Complete dissolution of IR-676 could be achieved within 10 to 20 min by vortexing and ultrasonication.…”

Section: Preparation Of the Ir-676-doped Micellesmentioning

confidence: 99%

Hydrophobic cyanine dye-doped micelles for optical in vivo imaging of plasma leakage and vascular disruption

et al. 2015

View full text Add to dashboard Cite

Abstract. Vascular leakage is an important feature of various disease conditions. In vivo optical imaging provides a great opportunity for the evaluation of this phenomenon. In the present study, we focus on the development and validation of a near-infrared (NIR) imaging formula to allow reliable, cost-efficient evaluation of vascular leakage in diverse species using the existing small-animal fluorescence imaging technology. IR-676, a moderately hydrophobic NIR cyanine dye, was doped into self-assembling aqueous micelles using a widely employed and safe nonionic emulsifier (Kolliphor HS 15), and was tested in several acute and chronic inflammatory disease models in both mice and rats. The imaging formula is stable and exerts no acute toxic effects in vitro. It accumulated specifically in the inflamed regions in all models, which could be demonstrated by both conventional epifluorescence imaging, and fluorescence tomography both as a standalone technique and also by merging it with computed tomography scans. Ex vivo verification of dye accumulation by confocal fluorescence microscopy was also possible. The present formula allows sensitive and specific detection of inflammatory plasma leakage in diverse models. Its potential for imaging larger animals was also demonstrated. IR-676-doped micelles offer an excellent opportunity to image inflammatory vascular leakage in various models and species.

show abstract

“…) was dissolved freshly in 5 w/v% aqueous solution of Kolliphor HS 15 and a macrogol-based surfactant (Kirchherr et al, 2009), and injected intravenously under ketamine e xylazine anesthesia (100 mg kg À1 and 5 mg kg…”

mentioning

confidence: 99%