Stabilization of galactose oxidase by high hydrostatic pressure: Insights on the role of cavities size
Min J. Kang,
José I. Reyes‐De‐Corcuera
Abstract:High hydrostatic pressure stabilized galactose oxidase (GaOx) at 70.0–80.0°C against thermal inactivation. The pseudo‐first‐order rate constant of inactivation kinact decreased by a factor of 8 at 80°C and by a factor of 44 at 72.5°C. The most pronounced effect of pressure was at the lowest studied temperature of 70.0°C with an activation volume of inactivation ΔV‡ of 78.8 cm3 mol−1. The optimal pressure against thermal inactivation was between 200 and 300 MPa. Unlike other enzymes, as temperature increased th… Show more
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