Stabilization of entrapped catalase using photo-crosslinked resin gel for use in wastewater containing hydrogen peroxide

Abstract

Catalase immobilized using photo-crosslinkable resin was applied to treatment of wastewater containing a low concentration of hydrogen peroxide. The structure of photo-crosslinked resin gel was stable even in the acceleration test of concentrated hydrogen peroxide for a long period. Accordingly, the most important subject of this process was maintaining long term catalase activity. The stabilization of immobilized catalase was investigated by modifying gel carriers with some prepolymers and functional monomers, and adding some stabilizing materials without modi®cation of catalase itself. But these gel carriers did not improve the stability beyond 20%. Addition of Tween 80, polyoxyethylene (5) sorbitan monooleate (hydrophilic/lipophilic balance, HLB: 10.0), to catalase was most effective for maintenance of the activity when the enzyme was immobilized in hydrophilic photocrosslinked resin, resulting in 80% of its initial activity. It was assumed that this surfactant did not in¯uence the permeation of hydrogen peroxide into gel, but acted as a stabilizer for catalase in the gel carrier.

Stabilization of α-amylase by using anionic surfactant during the immobilization process

“…This high stability suggests that very little leakage of the immobilized enzymes occurred under repeated washing. The mixing of AOT with a-amylase affected the enzyme activity by forming a protective layer of surfactant around the enzyme (Iida et al, 2000). Similar conclusions were reported by other workers with different enzymes, polymers, and crosslinkers (Yang, et al, 1995;Dessouki and Atia, 2002).…”

A kinetic analysis of catalytic production of oxygen in catalase‐containing liposome dispersions for controlled transfer of oxygen in a bioreactor

“…At very high enzyme concentrations, on the other hand, irreversible formation of aggregates consisting of thermally denatured enzymes is promoted . Catalase was stabilized by immobilization in solid supports . We reported that the quaternary structure and activity of bovine liver catalase were stabilized by encapsulating in phospholipid vesicles, i.e.…”