Stabilization of dry phospholipid bilayers and proteins by sugars

Crowe, John H.; Crowe, Lois M.; Carpenter, John F.; Wiström, Christina Aurell

doi:10.1042/bj2420001

Cited by 835 publications

(510 citation statements)

References 66 publications

(68 reference statements)

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“…The ability of carbohydrates to interact and protect membrane vesicles during freezing-thawing and drying has been documented (Strauss and Hauser, 1986;Crowe et al, 1987). This property is believed to be due to their capacity to interact directly with phospholipids in a manner which imitates the presence of water (Caffrey et al, 1988;Goodrich et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Introduction of Specific Carbohydrates into Eucalyptus gunnii Cells Increases their Freezing Tolerance

Leborgne

Teulières

Travert

et al. 1995

European Journal of Biochemistry

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The comparison of soluble sugar content in various cell lines of Eucalyptus gunnii exhibiting different freezing resistances revealed that the most resistant cell line contained the highest soluble sugar content. It was possible to increase the freezing resistance of the sensitive cell line by progressive exposure to low temperatures (acclimation). During the early stage of cold acclimation, an increase of soluble sugar concentration was observed in the cells confirming the correlation between freezing resistance and soluble carbohydrate content in this species. In addition, feeding experiments on the sensitive cell line were performed to introduce specific sugars into the cells. Both electropulsation and long‐term incubation in the presence of fructose and raffinose led to an increase in the tolerance of the cells during a freezing programme. Using radioactive fructose, the uptake of the sugar into cells and protoplasts was checked. In the light of these results, hypotheses are presented concerning the possible role of intracellular sugars in cryoprotection.

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Section: Discussionmentioning

confidence: 99%

Introduction of Specific Carbohydrates into Eucalyptus gunnii Cells Increases their Freezing Tolerance

Leborgne

Teulières

Travert

et al. 1995

European Journal of Biochemistry

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“…A proteção das células durante a fase de desidratação que ocorre durante a congelação e a estabilização da bicamada lipídica, como o impedimento da fusão de partículas intramembranosas, depende das substâncias que compõem o diluidor (Crowe et al, 1987).…”

Section: Introductionunclassified

Efeito de diferentes diluidores sobre a viabilidade espermática pós-descongelação de sêmen eqüino

Snoeck

Henry²,

Melo

2007

Arq. Bras. Med. Vet. Zootec.

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RESUMOEstudou-se o efeito de diferentes concentrações de gema de ovo, açúcares e tampões nos diluidores sobre a viabilidade espermática pós-descongelação de sêmen eqüino. Os diluidores foram: D1 = 10% de gema de ovo e acréscimo de 50% de lactose e glicose-EDTA em relação ao D5; D2= 10% de gema de ovo e redução de 50% de lactose e glicose-EDTA em relação ao D5; D3= 20% de gema de ovo e acréscimo de 50% de lactose e glicose-EDTA em relação ao D5; D4= 20% de gema de ovo e redução de 50% de lactose e glicose-EDTA em relação ao D5; D5= 20% de gema de ovo e composição de lactose e glicose-EDTA segundo Martin et al. (1979). Cinco ejaculados de seis garanhões foram utilizados para testar os diluidores contendo diferentes agentes crioprotetores: 3,5% de etilenoglicol e 5% de acetamida. Após diluição final nos diferentes diluidores, o sêmen foi submetido a diferentes protocolos de congelação: com e sem resfriamento prévio. Após descongelação em banho-maria a 75ºC, avaliaram-se a motilidade, o vigor, e a integridade estrutural e funcional das membranas espermáticas. A redução na concentração de gema de ovo de 20% para 10%, nos diluidores contendo a mesma concentração de açúcares e tampões, não alterou a capacidade crioprotetora dos diluidores com etilenoglicol ou acetamida. O aumento na concentração de açúcares e tampões no diluidor lactose-EDTA-gema de ovo não acrescentou efeito crioprotetor ao etilenoglicol e acetamida. Diluidores com maior osmolaridade tenderam a preservar melhor o sêmen eqüino congelado com etilenoglicol ou acetamida. Os melhores resultados de viabilidade espermática, pós-descongelação, foram para o sêmen congelado nos diluidores D1, D3 e D5 (P<0,05).Palavras-chave: eqüino, sêmen, criopreservação, diluidor ABSTRACTThe effect of different egg yolk, sugars and buffering systems concentrations in the freezability of differents equine semen extenders was studied. Extenders were: D1=10% of egg yolk added of more 50% over the lactose and glicose-EDTA levels found in D5; D2=10% of egg yolk and reduction of 50% over the lactose and glucose-EDTA levels in D5; D3=20% of egg yolk added of more 50% over the lactose and glucose-EDTA levels in D5; D4=20% of egg yolk and reduction of 50% over the lactose and glucose-EDTA levels in D5; D5=20% of egg yolk and lactose and glucose-EDTA according to Martin et al. (1979)

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“…[1,2] The biostabilizing properties of 1 are remarkable when compared with other simple sugars such as glucose and sucrose, making trehalose the excipient of choice for applications involving cryopreservation and anhydrobiosis. [3,4] Numerous investigations have been conducted to understand the basis for membrane stabilization by D-trehalose 1 and other excipients, many of which have been summarized in recent reviews. [1,2] Crowe and coworkers have collected ample evidence which point to two dominant factors: (1) depression of the gel phase transition temperature (T m ) of the cell membrane, which prevents cracking Supporting Information Full experimental details and chemical characterization of intermediates leading to L-trehalose 2 and meso-trehalose 3, DSC analyses of midpoint T g 's, and MTT assays examining the effect of exogenous trehalose during cold shock treatment prior to freezing (6 pages).…”

Section: Introductionmentioning

confidence: 99%

“…and leakage at low temperatures, and (2) formation of a vitrified state, which inhibits membrane fusion upon thawing or rehydration. [1,3,5] Phospholipid liposomes dried in the presence of 1 exhibit a depression in T m of 10−20 °C relative to their hydrated state, and can maintain a stable gel phase well below the freezing point of water. [5] With respect to vitrification, anhydrous 1 has a notably higher glass transition temperature (T g ) compared to other sugars, [6] a factor which is particularly relevant for membrane stabilization in a freeze-dried state.…”

Section: Introductionmentioning

confidence: 99%

Cryoprotection with L‐ and meso‐Trehalose: Stereochemical Implications

2006

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Two unnatural stereoisomers of α,α-trehalose (L-and meso-trehalose) were synthesized and evaluated as cryoprotectants, to determine the functional consequences of relative or absolute stereochemistry on their physicochemical properties. Adherent yeast cell cultures were frozen in 10% solutions of D-, L-, and meso-trehalose for periods of 7−28 days, then evaluated by a MTT viability assay. D-and Ltrehalose were equally effective in maintaining high rates of cell survival, demonstrating the absence of chiral discrimination at the carbohydrate-lipid interface, whereas meso-trehalose was inferior in cryoprotection efficacy. Differential scanning calorimetry revealed a difference in the glass transition temperatures (T g ) of D-and meso-trehalose of nearly 75 °C. This can be attributed to differences in conformational behavior, as portrayed by torsional energy maps for rotation about the glycosidic bonds of D-and meso-trehalose. We conclude that the biostabilizing properties of α,α-trehalose depend on relative stereochemical factors, but are independent of absolute stereochemical configuration.

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Stabilization of dry phospholipid bilayers and proteins by sugars

Cited by 835 publications

References 66 publications

Introduction of Specific Carbohydrates into Eucalyptus gunnii Cells Increases their Freezing Tolerance

Introduction of Specific Carbohydrates into Eucalyptus gunnii Cells Increases their Freezing Tolerance

Efeito de diferentes diluidores sobre a viabilidade espermática pós-descongelação de sêmen eqüino

Cryoprotection with L‐ and meso‐Trehalose: Stereochemical Implications

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