Stabilization of Candida rugosa lipase on nanosized zirconia-based materials

Guncheva, Maya; Paunova, Krasimira; Dimitrov, Momtchil; Yancheva, Denitsa

doi:10.1016/j.molcatb.2014.06.012

Cited by 16 publications

(1 citation statement)

References 42 publications

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“…[18][19][20][21][22][23][24][25] Nevertheless, the electronic properties of silica considerably limit its applicability in (bio)electrochemistry. 26 The development of well-structured nonsiliceous metal oxides 27,28 such as Nb 2 O 5 , 7 ZrO 2 , 29,30 ZnO, 31 and TiO 2 32,33 has led to a huge increase in the use of these materials.…”

Section: Introductionmentioning

confidence: 99%

The effect of the buffer solution on the adsorption and stability of horse heart myoglobin on commercial mesoporous titanium dioxide: a matter of the right choice

Loreto

Cuypers

Brokken

et al. 2017

Phys. Chem. Chem. Phys.

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Despite the numerous studies on the adsorption of different proteins onto mesoporous titanium dioxide and indications on the important role of buffer solutions in bioactivity, a systematic study on the impact of the buffer on the protein incorporation into porous substrates is still lacking. We here studied the interaction between a commercial mesoporous TiO and three of the most used buffers for protein incorporation, i.e. HEPES, Tris and phosphate buffer. In addition, this paper analyzes the adsorption of horse heart myoglobin (hhMb) onto commercial mesoporous TiO as a model system to test the influence of buffers on the protein incorporation behavior in mesoporous TiO. N sorption analysis, FT-IR and TGA/DTG measurements were used to evaluate the interaction between the buffers and the TiO surface, and the effect of such an interaction on hhMb adsorption. Cyclic voltammetry (CV) and electron paramagnetic resonance (EPR) were used to detect changes in the microenvironment surrounding the heme. The three buffers show a completely different interaction with the TiO surface, which drastically affects the adsorption of myoglobin as well as its structure and electrochemical activity. Therefore, special attention is required while choosing the buffer medium to avoid misguided evaluation of protein adsorption on mesoporous TiO.

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Section: Introductionmentioning

confidence: 99%

The effect of the buffer solution on the adsorption and stability of horse heart myoglobin on commercial mesoporous titanium dioxide: a matter of the right choice

Loreto

Cuypers

Brokken

et al. 2017

Phys. Chem. Chem. Phys.

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A novel biocatalytic system obtained via immobilization of aminoacylase onto sol–gel derived ZrO2·SiO2 binary oxide material: physicochemical characteristic and catalytic activity study

Kołodziejczak-Radzimska

Ciesielczyk

Jesionowski

2019

Adsorption

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Oxide material of a new type, ZrO 2 ·SiO 2 (raw and carbonyl-grafted), was used as support in the immobilization of aminoacylase from Aspergillus melleus. The ZrO 2 ·SiO 2 was synthesized via sol-gel method. The obtained material was additionally modified with glutaraldehyde. Various physicochemical analyses were used to confirm the effectiveness of the modification and immobilization processes, including Fourier transform infrared spectroscopy, laser Doppler velocimetry and lowtemperature N 2 sorption. The immobilization process was performed within 3 h using different concentrations of enzyme solution (1, 3, 5 and 7 mg/mL), and the Bradford method was used to determine the quantity of immobilized enzyme. The resulting biocatalytic systems were then used as catalysts in the hydrolysis of different N-acetyl-dl-amino acids (leading to l-methionine, l-cysteine, l-serine and l-tryptophan). Based on this reaction the apparent and relative catalytic activities were determined. The highest activity of the immobilized enzyme was attained in the synthesis of l-methionine (the apparent activities of aminoacylase immobilized on raw and carbonyl-grafted ZrO 2 ·SiO 2 were 4112 and 4947 U/g, respectively). Furthermore, the effect of pH and temperature on catalytic activity, as well as the storage stability and reusability of the prepared biocatalytic systems were determined. Aminoacylase immobilized on carbonyl-grafted ZrO 2 ·SiO 2 retains 85% of its initial activity after 30 days of storage and 70% after five reaction cycles.

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Synthesis and Characterization of Chitosan/TiO2 Composite Beads for Improving Stability of Porcine Pancreatic Lipase

Deveci

Doğaç

Teke

et al. 2014

Appl Biochem Biotechnol

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The purpose of the present work is improving stability properties of porcine pancreatic lipase (triacylglycerol lipase, E.C.3.1.1.3) by immobilization on chitosan/TiO2 composite beads. The immobilization parameters were initial enzyme concentration (0.5-2 mg/ml), adsorption time (5-25 min), and glutaraldehyde concentration (1-4 % v/v). The optimum temperature (20-60 °C), optimum pH (3.0-10.0), kinetic parameters, thermal stability (4-70 °C), pH stability (4.0-9.0), and reusability (9 times) were investigated for characterization of immobilized lipase system. The optimum temperatures of free and immobilized lipase were 30 °C. The temperature profile of the immobilized lipase was spread over a large area. The optimum pH values for the free lipase and immobilized lipase were found to be 6.5 and 7.5, respectively. The thermal stability of immobilized lipase was evaluated, and it maintained 45 % activity at 70 °C. But, at this temperature, soluble lipase protected only 15 % activity. Also, the structural characterization of chitosan/TiO2 composite beads was analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy analysis (ATR-FTIR). The significance of this study is improving of stability properties of lipase for the industrial usage especially production of biodiesel and dairy products.

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Stabilization of Candida rugosa lipase on nanosized zirconia-based materials

Cited by 16 publications

References 42 publications

The effect of the buffer solution on the adsorption and stability of horse heart myoglobin on commercial mesoporous titanium dioxide: a matter of the right choice

The effect of the buffer solution on the adsorption and stability of horse heart myoglobin on commercial mesoporous titanium dioxide: a matter of the right choice

A novel biocatalytic system obtained via immobilization of aminoacylase onto sol–gel derived ZrO2·SiO2 binary oxide material: physicochemical characteristic and catalytic activity study

Synthesis and Characterization of Chitosan/TiO2 Composite Beads for Improving Stability of Porcine Pancreatic Lipase

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