Stabilization of Ca-deficient hydroxyapatite in biphasic calcium phosphate ceramics by adding alginate to enhance their biological performances

Li, Xiangfeng; Deng, Yun; Wang, Menglu; Chen, Xuening; Xiao, Yumei; Zhang, Xingdong

doi:10.1039/c7tb02620j

Cited by 34 publications

(44 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As Fig. 11, bone-like apatite deposits [58,59] cover the entire surface of the HT coatings and new morphology of deposited particles is found, called sponge-like HA [60]. This extreme increase in bioactivity maybe is related to the plate size decreasing and the appearance of needle-like morphology that prepares more effective contact surface with SBF.…”

Section: In-vitro Testmentioning

confidence: 96%

Synthesizing and comparing HA–TiO2 and HA–ZrO2 nanocomposite coatings on 316 stainless steel

Poorraeisi

Afshar

2019

SN Appl. Sci.

View full text Add to dashboard Cite

In this research, hydroxyapatite coating and composite hydroxyapatite coatings containing nanoparticles were fabricated using a single step electrodeposition method. To investigate the effect of compositing agent type on hydroxyapatite structure, two different compounds (titania and zirconia) were used. Each compound was used at two different concentrations to observe the effect of compositing agent content on matrix structure. Pure hydroxyapatite was deposited with a water based Calcium-Phosphate (Ca-P) solution with pH = 4.2. To deposit composite coatings, a merged solution was prepared by merging CaP solution with aqueous TiO 2 (ZrO 2) suspension. Prepared coatings were analyzed using thickness measurement, XRD, FTIR, FESEM, polarization test and immersion in SBF solution. In the first step, XRD, FTIR and FESEM results proved the formation of hydroxyapatite using CaP solution and analysis of composite coatings proves the existence of nanoparticles in the coatings as compositing agent and the coating procedure was successful. Thereafter, polarization and immersion results indicate that composite coatings show superior properties than pure hydroxyapatite. The properties of hydroxyapatite matrix are influenced not only by the type of the compositing agent but also by the content of the additive. Adding nanoparticles changes different characteristics of pure HA, such as (1) decreasing the thickness of the coating, pure hydroxyapatite was 5.5 µm and the thickness of HA-ZrO 2 (ZrO 2 : 6 mM = HZ 2) composite coating was 4.4 µm; (2) increasing the crystallinity (Xc), Xc for HZ 2 coating is 86% and Xc for pure HA is 80%; (3) obvious changes in morphology has occurred and new morphologies of HA are observed which has not been mentioned in the literature previously; (4) composite coatings have more positive E corr than pure HA, E corr. of HZ 2 coating is − 0.23 which is the most positive one and E corr. of HA is − 0.41; (5) composite coatings have smaller porosity ratio than pure HA; (6) Pure and composite coatings are bioactive, but composite coatings show different bioactivity behavior.

show abstract

Section: In-vitro Testmentioning

confidence: 96%

Synthesizing and comparing HA–TiO2 and HA–ZrO2 nanocomposite coatings on 316 stainless steel

Poorraeisi

Afshar

2019

SN Appl. Sci.

View full text Add to dashboard Cite

show abstract

“…Due to the addition of alginate calcium, the grain size of BCP-SA (360.86 ± 208.44 nm) was significantly smaller than that of BCP, which was consistent with our previous work. 20 The grain sizes of BCP-1%CaO, BCP-2%CaO, BCP-3%CaO, and BCP-4%CaO were decreased with the increase of CaO content. BCP-1%CaO had the largest grain size of 631.08 ± 163.95 nm among the four BCP-CaO samples.…”

Section: Discussionmentioning

confidence: 89%

“…Consistent with our previous work. 20 the HA phase was increased to 84.7% in BCP-SA with the addition of alginate calcium, and the lattice parameters (ie, a/b-axis, c-axis and cell volume) of HA phase and β-TCP phase in BCP-SA were higher than those in BCP, while the crystal size of in BCP-SA was smaller than the one in BCP. In BCP ceramics with CaO addition, HA phase increased with the increase of CaO content, as the HA phase contents in BCP-1%CaO, BCP-2%CaO, BCP-3%CaO, and BCP-4%CaO were 45.5%, 59.6%, 92.6%, and 95.1%, respectively.…”

Section: Phase Compositions and Ca/p Ratiosmentioning

confidence: 92%

“…The quantitative ALP activity was tested colorimetrically using Alkaline Phosphatase Assay Kit (Beyotime, China) as previous described. 7,20 To investigate the mineralization of the BMSCs on the obtained samples, Alizarin Red S (Alfa Aesar, USA) were conducted according to the manufacturer's procedures, and the staining was also semi-quantified using ImageJ software. Moreover, after 7 days of culture, BMSCs cultured on the samples were…”

Section: Alp Activity Mineralization Assay and Opn Immunofluorescmentioning

confidence: 99%

“…18 In fact, the initial BCP precursor is composed of CDHA phase, but CDHA will decompose into HA phase and β-TCP phase in the sintering process of BCP ceramics, so it is generally difficult to find CDHA phase in BCP ceramics after sintering. 19,20 In 2018, our group suggested that the decomposition products (ie CaO) of calcium alginate might react with ceramic precursor to stabilize the CDHA phase during the sintering process. Thus, the addition of calcium alginate could stabilize CDHA, improve the microporosity, and decrease the grain size in BCP ceramics, resulting in the enhanced osteogenic activity and osteoinductivity.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Enhancing mechanical and biological properties of biphasic calcium phosphate ceramics by adding calcium oxide

Wang

Chen

et al. 2020

J. Am. Ceram. Soc.

Self Cite

View full text Add to dashboard Cite

Biomaterials can induce the regeneration of damaged tissue through the optimized design of the materials themselves, which is important to the development of regenerative medicine. 1-3 Calcium phosphate (Ca-P) ceramics are widely believed as excellent bone grafts, in consideration of their good biocompatibility, bioactivity, and osteoconductivity. 4-6 Among them, biphasic calcium phosphate (BCP) ceramic seems to be one of the best choices, due to its suitable solubility and excellent osteoinductivity. 7-9 However, traditional BCP ceramics are facing many challenges to meet the requirements of regenerative medicine. For instance, BCP ceramics still have relatively low biological properties as comparing compared to natural bones. 10,11 Further mimicking the bony composition and structure to enhance the bioactivity of BCP ceramics seems necessary and significative. In addition, the brittleness and low resistance to fracture of BCP ceramics limit their applications in non-load-bearing bone defect filling, and thus the

show abstract

A Novel Hollow‐Tube‐Biphasic‐Whisker‐Modified Calcium Phosphate Ceramics with Simultaneously Enhanced Mechanical Strength and Osteogenic Activity

Feng

et al. 2022

Adv Funct Materials

View full text Add to dashboard Cite

Due to the inherent brittleness and low mechanical strength, it is still a challenge for calcium phosphate (Ca-P) ceramics to be used in load-bearing bone defect repair. To achieve a good balance between mechanical strength and osteogenic activity, hollow-tube-whisker-modified biphasic calcium phosphate (BCP) ceramics (BCP-HW) are successfully fabricated by an in situ growth process in the present study. Compared to the initial BCP ceramics (BCP-C) and those with solid whiskers, BCP-HW exhibits larger specific surface area (3.9 times vs BCP-C) and higher mechanical strength (3.4 times vs BCP-C), endowing it with stronger stimulation on adhesion, proliferation, and osteogenic differentiation of bone marrow mesenchymal stem cells. In an intramuscular implantation model of canine, BCP-HW shows excellent osteoinductivity and promotes the maturation of new bone, and the resultant compressive strength of the implant increases to ≈12 MPa at 3 months postoperatively. In another critical-sized segmental bone defect model of rabbit femur, BCP-HW has the best repairing effect. After implantation for 6 months, much more new bone ingrowth and higher bending load are observed in BCP-HW than BCP-C. Collectively, these findings suggest that the in situ hollow-tube whisker construction possesses immense potential in expanding the applications of Ca-P ceramics to load-bearing bone defect repair.

show abstract

Stabilization of Ca-deficient hydroxyapatite in biphasic calcium phosphate ceramics by adding alginate to enhance their biological performances

Abstract: It is of significance to further improve the bioactivity of existing calcium phosphate (Ca–P) biomaterials to satisfy the needs of regenerative medicine.

Cited by 34 publications

References 47 publications

Synthesizing and comparing HA–TiO2 and HA–ZrO2 nanocomposite coatings on 316 stainless steel

Synthesizing and comparing HA–TiO2 and HA–ZrO2 nanocomposite coatings on 316 stainless steel

Enhancing mechanical and biological properties of biphasic calcium phosphate ceramics by adding calcium oxide

A Novel Hollow‐Tube‐Biphasic‐Whisker‐Modified Calcium Phosphate Ceramics with Simultaneously Enhanced Mechanical Strength and Osteogenic Activity

Contact Info

Product

Resources

About