Stabilization of all-trans-lycopene from tomato by encapsulation using cyclodextrins

Abstract

The stabilization of all-trans-lycopene from tomato by encapsulation using a-, b-and c-cyclodextrins (CDs) was evaluated. To that end, two different encapsulation methods were comparatively studied: a conventional method and a supercritical fluid extraction (SFE) process. An optimization procedure considering distinct molar ratios of CD/lycopene (1/0.0026, 1/0.005 and 1/0.05) as well as the type of cyclodextrin to be used was accomplished. The encapsulation was determined by using micro-Raman spectroscopy. All-trans-lycopene employed was obtained by SFE with a purity around 90-95%. As a result, a molar ratio CD/lycopene of 1/0.0026 was selected as it provided the best complexation yields (93.8%) whilst b-CD seemed to be the most favorable to be used to stabilize lycopene. A comparison of the two methods studied reflected higher encapsulation yields from the conventional method. However, the supercritical fluid approach offers numerous advantages such as the possibility of performing the extraction, fractionation and encapsulation of lycopene from tomato in one step, shortening notably the overall procedure time and minimizing the sample handling.

Preparation of inclusion complex of piroxicam with cyclodextrin by using supercritical carbon dioxide

“…Drug References geraniol, mustard oil [12] acetaminophen [13] piroxicam [14,15] miconazole [15,16,17,18,19,20] Eflucimibe [21,22] ibuprofen [23,24,25,26,27,28,29] naproxen [28,30,31,32] flurbiprofen [28] citral, thymol, carvacrol [33] imazalil [34] salicylic acid [35] azobenzene [36] budesonide [37,38,39] indomethacin [40] polyaniline [41] cholesterol [42] arylphosphines [43] itraconazole [44,45,46] 2 sodium salts [47] hydroxyflavone [48] benzocaine [50, 49,] bupivacaine, and mepivacaine [50] simvastatin [51] several triphenyl phosphine derivatives [52] all-trans-lycopene [53] 15 carbohydrates [54] human serum immunoglobulin G …”

Carotenes and carotenoids in natural biological samples: a Raman spectroscopic analysis

“…In a very recent article, Blanch et al [55] have investigated the stabilization of an all-trans-lycopene obtained from tomatoes by encapsulation using α, β and γ -cyclodextrin. In addition to the demonstrated stabilization of lycopene encapsulated by cyclodextrins, it was also very clear that the carotenoid showed a very significant wavenumber shift mainly for the C C bond stretch which indicated that the carotenoid undergoes a structural change upon complexation with the cyclodextrin.…”

Carotenoid Pigments Encapsulation: Fundamentals, Techniques and Recent Trends

“…Additionally, others drawbacks about this method that is necessary to mention is its very expensive and rather complex process [33]. Recently alternative technologies and/or even some small modification as change the conventional organic solvent to "greener solvent" such as supercritical fluids have been evaluated to eliminate some limitations of the conventional encapsulation methods [34].…”