Stabilization and Controlled Release of Microencasulated Hydrogen Peroxide for Wound Treatment Applications

Dogan‐Guner, E. M.; Mohamed, Hagir; Orbey, Neşe; Goodyear, Nancy

doi:10.1111/jam.14177

Cited by 7 publications

(7 citation statements)

References 21 publications

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“…A slow release of H 2 O 2, at low concentrations, may prevent bacterial infections in wounds, and H 2 O 2 at low concentrations is more stable. In another study, a continuous and slow release of H 2 O 2 in diluted honey allowed the development of a hydrogel to achieve a continuous, controlled release of H 2 O 2 for up to 72 h [ 55 ]. Also, H 2 O 2 micro-encapsulated hydrogels demonstrated broad-spectrum antimicrobial activity with as little as 10 min of contact time [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Characterisation of physicochemical parameters and antibacterial properties of New Caledonian honeys

Bucekova,

Godocikova,

Gueyte

et al. 2023

PLoS ONE

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Honey is an attractive natural product with various health benefits. A few honey-based commercial products have successfully been adopted in clinics to improve wound healing. However, screening of other potential sources of medical-grade honey, in particular, honeys from territories with high floral species diversity and high endemicity, is highly needed. The goal of this study was to characterise the physicochemical and antibacterial properties of New Caledonian honey samples (n = 33) and to elucidate the major mechanism of their antibacterial action. Inhibitory antibacterial activity of honeys against Staphylococcus aureus and Pseudomonas aeruginosa was determined with a minimum inhibitory concentration (MIC) assay. Enzymatic activity of glucose oxidase and the content of hydrogen peroxide (H2O2) in honey samples were analysed. Furthermore, total protein content of honeys together with their electrophoretic protein profiles were also determined in the study. The antibacterial efficacy of 24% of the tested honey samples was slightly superior to that of manuka honey with unique manuka factor 15+. The antibacterial activity of catalase-treated honey sample solutions was significantly reduced, suggesting that H2O2 is a key antibacterial compound of diluted honeys. However, the kinetic profiles of H2O2 production in most potent honeys at a MIC value of 6% was not uniform. Under the experimental conditions, we found that a H2O2 concentration of 150 μM in diluted honeys is a critical concentration for inhibiting the growth of S. aureus. In contrast, 150 μM H2O2 in artificial honey solution was not able to inhibit bacterial growth, suggesting a role of phytochemicals in the antibacterial activity of natural honey. In addition, the continuous generation of H2O2 in diluted honey demonstrated an ability to counteract additional bacteria in re-inoculation experiments. In conclusion, the tested New Caledonian honey samples showed strong antibacterial activity, primarily based on H2O2 action, and therefore represent a suitable source for medical-grade honey.

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Section: Discussionmentioning

confidence: 99%

Characterisation of physicochemical parameters and antibacterial properties of New Caledonian honeys

Bucekova,

Godocikova,

Gueyte

et al. 2023

PLoS ONE

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“…Depending on several factors but mainly on its concentration, H 2 O 2 may exert either bacteriostatic or bactericidal effects. H 2 O 2 at low concentrations, that are more stable than higher concentrations, exerts weak antibacterial activity; however, extended slow-release of H 2 O 2 may improve its antibacterial activity and overwhelm the effect of bacterial/plant catalase [25].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Different Blossom Honeys: New Findings

et al. 2019

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Antibacterial activity is the most investigated biological property of honey. The goal of this study was to evaluate the antibacterial activity of 57 Slovak blossom honeys against Staphylococcus aureus and Pseudomonas aeruginosa and investigate the role of several bioactive substances in antibacterial action of honeys. Inhibitory and bactericidal activities of honeys were studied to determine the minimum inhibitory and bactericidal concentrations. The contents of glucose oxidase (GOX) enzyme, hydrogen peroxide (H2O2), and total polyphenols (TP) were determined in honeys. We found that honey samples showed different antibacterial efficacy against the tested bacteria as follows: wildflower honeys > acacia honeys > rapeseed honeys. Overall antibacterial activity of the honeys was statistically-significantly correlated with the contents of H2O2 and TP in honeys. A strong correlation was found between the H2O2 and TP content. On the other hand, no correlation was found between the content of GOX and level of H2O2. Antibacterial activity of 12 selected honeys was markedly reduced by treatment with catalase, but it remained relatively stable after inactivation of GOX with proteinase-K digestion. Obtained results suggest that the antibacterial activity of blossom honeys is mainly mediated by H2O2 levels present in honeys which are affected mainly by polyphenolic substances and not directly by GOX content.

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“…Recently, researchers have suggested that polyphenols could be involved in the generation of hydrogen peroxide at low concentration by autoxidation of polyphenols ( Bucekova et al, 2019 , Grzesik et al, 2019 ), and the slow release of

in honey may improve its antibacterial activity ( Dogan-Guner et al, 2018 ). A new finding documented a significant correlation between the

and total phenol content in the overall antibacterial activity of the honey ( Grzesik et al, 2019 )…”

Section: Discussionmentioning

confidence: 99%

Potential effects of Saudi Shaoka (Fagonia bruguieri) honey against multi-drug-resistant bacteria and cancer cells in comparison to Manuka honey

Halawani

2021

Saudi Journal of Biological Sciences

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The global spread of antimicrobial-resistant infectious diseases and cancer are the most widespread public health issue and has led to high mortality rates. This study aims to evaluate and verify the antibacterial and antitumor activities of Shaoka and Manuka honey against pathogenic bacteria, human hepatocarcinoma (HepG2) and breast cancer (MCF-7) cell lines. Shaoka hone was analyzed using HPLC, UV–vis, and GC/MC, while antibacterial activity was measured by agar diffusion, broth microdilution methods, and Transmission Electron Microscopy (TEM). Antitumor activity was investigated morphologically and by MTT assay. According to the presented data of HPLC analysis, Shaoka honey was generally richer in polyphenolic components, the antibacterial activity showed that Shaoka honey is equivalent or relatively more active than Manuka honey against a broad spectrum of multi-drug-resistant bacteria. It inhibited the growth of ESBL Escherichia coli in the absence or presence of catalase enzyme with a concentration approximately 8.5%–7.3% equivalent to phenol, which supported the highest level of non-peroxide-dependent activity. The minimum bactericidal concentrations (MBCs) ranged between 5.0% and 15.0% honey (w/v). TEM observation revealed distorted cell morphology, cytoplasmic shrinkage, and cell wall destruction of treated bacteria. The selected honey exerted cytotoxicity on both cancer cell lines, inhibiting cell proliferation rate and viability percent in HepG2 and MCF-7 cancer cells, by different degrees depending on the honey quality, Shaoka honey competed Manuka inhibitory effects against both cancer cells. The obtained data confirmed the potential for use of Saudi Shaoka honey as a remedy, this well introduces a new honey template as medical-grade honey for treating infectious disease and cancer.

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Stabilization and Controlled Release of Microencasulated Hydrogen Peroxide for Wound Treatment Applications

Cited by 7 publications

References 21 publications

Characterisation of physicochemical parameters and antibacterial properties of New Caledonian honeys

Characterisation of physicochemical parameters and antibacterial properties of New Caledonian honeys

Antibacterial Activity of Different Blossom Honeys: New Findings

Potential effects of Saudi Shaoka (Fagonia bruguieri) honey against multi-drug-resistant bacteria and cancer cells in comparison to Manuka honey

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