Stability Study on Specimens Mailed to a State Laboratory and Tested With the Gen-Probe PACE 2 Assay for Chlamydia

Parker, Elizabeth; Wozniak, Arthur; White, Sue D.; Beckham, Cathy; Roberts, Dixie

doi:10.1097/00007435-199904000-00005

Cited by 7 publications

(4 citation statements)

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“…This is supported by studies describing the effects of storage and shipping conditions on test outcome for Chlamydia spp. 2,11 Although temperature variation en route was not assessed for either shipping method in the present study, it seems reasonable to assume that couriered samples experienced less variation in temperature and less extreme temperatures and for shorter times than did postal samples. Although the highest temperature measured at the time of unpacking a sample sent by USPS in this study was 24uC, maximum ambient temperatures at the shipping and receipt cities were 47uC and 41uC, respectively.…”

mentioning

confidence: 76%

Effects of Temperature and Time in Transit on Polymerase Chain Reaction Detection of Feline Herpesvirus DNA

2006

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Abstract. The purpose of this study was to report methods currently recommended by commercial laboratories for collecting, shipping, and processing of samples for feline herpesvirus type 1 (FHV-1) testing using polymerase chain reaction (PCR) and to determine the effect of temperature and time on the ability of 1 PCR method to detect FHV-1 DNA in experimental and clinical samples. Eleven laboratories offering FHV-1 PCR were surveyed. There was notable variation in sample types and shipping conditions recommended and PCR protocols used by these laboratories. Subsequently, using a single PCR method, FHV-1 DNA was detected in samples exposed to various temperatures within the laboratory. Finally, FHV-1 PCR was performed on paired clinical samples collected from 25 cats and shipped at ambient temperatures via US Postal Service (USPS) or with an ice pack via a courier. Samples sent by USPS were exposed to significantly longer transit time and arrived at significantly higher temperature than did samples sent by courier. Despite this, all sample pairs yielded concordant results when tested for FHV-1 DNA using this PCR method. Although it may not be necessary for samples collected for detection of FHV-1 DNA using this PCR method to be shipped under the most expedient or temperature-controlled conditions, this should be verified for a variety of PCR assays and sample types.

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confidence: 76%

Effects of Temperature and Time in Transit on Polymerase Chain Reaction Detection of Feline Herpesvirus DNA

2006

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“…Recent studies have shown that specimens are not adversely affected by heat and extended transit times. 10 The DH has an approximate turnaround time of ten days. In cases with a positive result, the DH staff contact our ED quality assurance coordinator by fax or telephone, who confirms by chart review that the patient received appropriate antibiotic therapy.…”

Section: Methodsmentioning

confidence: 99%

Follow‐up Program for Emergency Department Patients with Gonorrhea or Chlamydia

Kelly

Dalsey

McComb

et al. 2000

Academic Emergency Medicine

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Abstract. Objectives: To study the performance of a centralized regional follow-up program organized by a municipal department of health (DH) for female patients presenting to the emergency department (ED) with Neisseria gonorrhoeae and/or Chlamydia trachomatis, who are not diagnosed or treated at the time of presentation. Methods: This was a retrospective observational study of female patients seen in the ED with positive cervical specimens, and their subsequent treatment and follow-up by the DH. Medical records were reviewed to determine the female patients seen in the ED who had positive specimens for N. gonorrhoeae or C. trachomatis. The DH followed up those not treated in the ED. Analysis of how long it took for these patients to be treated and the proportion lost to follow-up was performed. Results: Of 2,121 specimens, 342 were positive for N. gonorrhoeae or C. trachomatis. Of the 342, 154 (45%) were recognized and appropriately treated in the ED. One hundred fifty-nine of the 342 (46.5%) patients were discharged from the ED without treatment but were contacted by the DH and appropriate treatment was provided. The DH could not locate 23 (6.7%) patients, and four (1.2%) refused treatment. One died before treatment. Only 21 of the 159 were treated within nine days. Median time to treatment was 36 days. Conclusions: Centralized laboratory analysis and follow-up by the DH for N. gonorrhoeae and C. trachomatis identified many female patients undiagnosed and untreated in the ED. The DH follow-up program provided appropriate treatment to most female patients.

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“…As a result, there has been a shift to centralized or consolidated laboratory services that has occurred on both a regional and a national scale (7). Although the issue of transit times for clinical specimens to reach a central laboratory has become much more important, there have been very few investigations of the impact of delays (6). Bacteria that are particularly sensitive to ambient conditions include Shigella spp., Neisseria gonorrhoeae, Neisseria meningitidis, S. pneumoniae, H. influenzae, and anaerobes (9).…”

Section: Streptococcus Pneumoniae Haemophilus Influenzae Andmentioning

confidence: 99%

Comparison between Nasal Swabs and Nasopharyngeal Aspirates for, and Effect of Time in Transit on, Isolation of Streptococcus pneumoniae , Staphylococcus aureus , Haemophilus influenzae , and Moraxella catarrhalis

et al. 2007

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We assessed the impact of the use of nasal swabs or nasopharyngeal aspirates and the time from specimen collection to storage at ؊70°C on bacterial isolation. Haemophilus influenzae was isolated significantly less often from swabs than from nasopharyngeal aspirates. Samples in transit for >3 days were half as likely to grow Streptococcus pneumoniae and H. influenzae as those in transit for <3 days. There was no statistically significant difference for either Moraxella catarrhalis or Staphylococcus aureus.

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Stability Study on Specimens Mailed to a State Laboratory and Tested With the Gen-Probe PACE 2 Assay for Chlamydia

Cited by 7 publications

References 0 publications

Effects of Temperature and Time in Transit on Polymerase Chain Reaction Detection of Feline Herpesvirus DNA

Effects of Temperature and Time in Transit on Polymerase Chain Reaction Detection of Feline Herpesvirus DNA

Follow‐up Program for Emergency Department Patients with Gonorrhea or Chlamydia

Comparison between Nasal Swabs and Nasopharyngeal Aspirates for, and Effect of Time in Transit on, Isolation of Streptococcus pneumoniae , Staphylococcus aureus , Haemophilus influenzae , and Moraxella catarrhalis

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