Stability study of 81 analytes in human whole blood, in serum and in plasma

Oddoze, Christiane; Lombard, Elise; Portugal, Henri

doi:10.1016/j.clinbiochem.2012.01.012

Cited by 243 publications

(189 citation statements)

References 18 publications

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“…• C. U-CYSC was determined in duplicates from freshly obtained urine samples and after 2, 4, 6, 24 h of storage [20]. Additionally, the effects of 3 months' storage at -20…”

Section: Samplementioning

confidence: 99%

Novel Automated Immune Turbidimetric Assay for Routine Urinary Cystatin-C Determinations

et al. 2018

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Aim: There is no commercially available urinary cystatin-C (u-CYSC) test in the market. Therefore, we optimized and validated an automated immune turbidimetric test for u-CYSC measurements and investigated u-CYSC concentrations in acute and chronic diseases which might lead to renal tubular disorders. Materials & methods: A particle-enhanced immune turbidimetric assay was adapted and validated on a Cobas 8000/c502 analyzer. Urine samples of different patient groups were also analyzed. Results: Our method showed excellent analytical performance. U-CYSC/u-creatinine (u-CREAT) was higher in sepsis-related acute kidney injury group (p < 0.001) compared with controls and to patients with chronic hypertension and Type 2 diabetes. Conclusion: We validated a fast, sensitive, fully automated u-CYSC assay which is ideal for routine use and might be a potential complementary laboratory test to evaluate renal tubular function.

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“…• C. U-CYSC was determined in duplicates from freshly obtained urine samples and after 2, 4, 6, 24 h of storage [20]. Additionally, the effects of 3 months' storage at -20…”

Section: Samplementioning

confidence: 99%

Novel Automated Immune Turbidimetric Assay for Routine Urinary Cystatin-C Determinations

et al. 2018

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“…Fifteen papers relating to PTH stability in whole blood were identified that had used a second generation assay ( Figure 2, Supplemental Data Table 3) [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29]. Collation of study results was difficult due to variation in experimental design (see above), but almost all studies suggested that PTH was more stable in whole blood samples containing EDTA than in anticoagulant-free samples (Supplemental Data Table 3).…”

Section: Stability In Anticoagulated and Clotted Whole Blood At Room mentioning

confidence: 99%

Sampling and storage conditions influencing the measurement of parathyroid hormone in blood samples: a systematic review

Hanon

Sturgeon

Lamb

2013

Clinical Chemistry and Laboratory Medicine (CCLM)

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Parathyroid hormone (PTH) is relatively unstable: optimisation of pre-analytical conditions, including specimen type, sampling time and storage conditions, is essential. We have undertaken a systematic review of these pre-analytical conditions. An electronic search of the PubMed, Embase, Cochrane, Centre for Research and Dissemination and Bandolier databases was undertaken. Of 5511 papers identified, 96 underwent full text review, of which 83 were finally included. At room temperature PTH was stable in ethylenediaminetetraacetic acid (EDTA) preserved whole blood for at least 24 h and in EDTA plasma for at least 48 h after venepuncture. Losses were observed in clotted blood samples after 3 h and in serum after 2 h. At 4°C PTH was more stable in EDTA plasma (at least 72 h) than serum (at least 24 h). Central venous PTH concentrations were higher than peripheral venous concentrations. In the northern hemisphere, PTH concentrations were higher in winter than summer. PTH has a circadian rhythm characterised by a nocturnal acrophase and mid-morning nadir. Data related to frozen storage of PTH (−20°C and −80°C) were limited and contradictory. We recommend that blood samples for PTH measurement should be taken into tubes containing EDTA, ideally between 10:00 and 16:00, and plasma separated within 24 h of venepuncture. Plasma samples should be stored at 4°C and analysed within 72 h of venepuncture. Particular regard must be paid to the venepuncture site when interpreting PTH concentration. Further research is required to clarify the suitability of freezing samples prior to PTH measurement.

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“…The decreases in glucose concentration seem to be dependent on both time and temperature, and result in an increased lactate concentration [22]. In contrast, insulin concentration was in a previous study stable for six hours in room temperature, and 72 hours at 4ºC [23]. The stability of endogenous glucose and insulin concentrations in human whole blood at different temperatures is not sufficiently quantified in previous studies; neither is the possible effect of insulin and endotoxin addition.…”

Section: Introductionmentioning

confidence: 81%

“…Wilcoxon signed rank test revealed that endogenous insulin concentration after incubation at 37 ºC for 6 hours was significantly reduced from baseline 48 (36-94) to 23 (18)(19)(20)(21)(22)(23)(24)(25)(26)(27) pmol/L (p=0.03) (Figure 2). …”

Section: Insulin Concentrationsmentioning

confidence: 99%

Reduction of glucose and insulin concentrations during in vitro incubation of human whole blood at different temperatures

Beitland

Opdahl²,

Aspelin³

et al. 2013

J Diab Res Clin Met

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Background: Incubation of human whole blood at body temperature has been used in numerous studies without addition of glucose and insulin. The purpose of the study was to examine glucose concentration changes during in vitro incubation of human whole blood at different temperatures, and whether it was affected by addition of insulin and bacterial endotoxin. We also wanted to quantify changes in endogenous insulin concentrations during incubation for six hours at 37 ºC. Methods: Young, healthy and fasting males donated whole blood. Glucose concentrations were compared at baseline and after six hours incubation at 37 ºC, 22 ºC or 0 ºC, in aliquots with and without addition of insulin and bacterial endotoxin. Glucose data are presented as mean (± 1 standard deviation). Endogenous insulin concentrations in aliquots without addition were measured at baseline and after six hours incubation at 37 ºC, data are shown as median (interquartile range). Results: Glucose concentration at baseline was 5.3 (± 0.6) mmol/L. After incubation for six hours at different temperatures, the glucose level at 37 ºC was 1.0 (± 0.5) mmol/L (p<0.01), at 22 ºC 3.0 (± 0.6) mmol/L (p<0.01), and at 0 ºC 5.4 (± 0.7) mmol/L (p=0.95). The decline in glucose concentration seemed to be independent of addition of insulin and bacterial endotoxin. Endogenous insulin levels decreased from baseline 48 (36-94) pmol/L to 23 (18-27) pmol/L (p=0.03) during six hours incubation at 37 ºC. Conclusions: Glucose concentration was markedly reduced during in vitro incubation of whole blood from healthy volunteers for six hours at 37 ºC and 22 ºC, but was maintained at 0 ºC. Endogenous insulin level after six hours incubation of whole blood at 37 ºC was more than halved compared to baseline. During in vitro studies of glucose and/or insulin effects lasting for hours, measures must be undertaken to maintain stable glucose and/or insulin concentrations.

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Stability study of 81 analytes in human whole blood, in serum and in plasma

Cited by 243 publications

References 18 publications

Novel Automated Immune Turbidimetric Assay for Routine Urinary Cystatin-C Determinations

Novel Automated Immune Turbidimetric Assay for Routine Urinary Cystatin-C Determinations

Sampling and storage conditions influencing the measurement of parathyroid hormone in blood samples: a systematic review

Reduction of glucose and insulin concentrations during in vitro incubation of human whole blood at different temperatures

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