Stability of the pBR322 plasmid derivative pBB210 in <i>Escherichia coli</i> TG1 under non‐selective and selective conditions

Löser, Christian

doi:10.1002/abio.370150411

Cited by 5 publications

(4 citation statements)

References 6 publications

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“…Some ARGs could readily disappear in natural systems, , but factors apart from antibiotics could increase the persistence of ARGs including the presence of metals or xenobiotic organic pollutants, , which are commonly elevated in MSW and landfill leachates . As shown in Figure , the most commonly detected ARGs were sul2 and ermB averaging at −1.37 ± 1.1 and −1.…”

Section: Resultsmentioning

confidence: 97%

Relationships between Antibiotics and Antibiotic Resistance Gene Levels in Municipal Solid Waste Leachates in Shanghai, China

Huang

Yang

et al. 2015

Environ. Sci. Technol.

267

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Many studies have quantified antibiotics and antibiotic resistance gene (ARG) levels in soils, surface waters, and waste treatment plants (WTPs). However, similar work on municipal solid waste (MSW) landfill leachates is limited, which is concerning because antibiotics disposal is often in the MSW stream. Here we quantified 20 sulfonamide (SA), quinolone (FQ), tetracycline (TC), macrolide (ML), and chloramphenicol (CP) antibiotics, and six ARGs (sul1, sul2, tetQ, tetM, ermB, and mefA) in MSW leachates from two Shanghai transfer stations (TS; sites Hulin (HL) and Xupu (XP)) and one landfill reservoir (LR) in April and July 2014. Antibiotic levels were higher in TS than LR leachates (985 ± 1965 ng/L vs 345 ± 932 ng/L, n = 40), which was because of very high levels in the HL leachates (averaging at 1676 ± 5175 ng/L, n = 40). The mean MLs (3561 ± 8377 ng/L, n = 12), FQs (975 ± 1608 ng/L, n = 24), and SAs (402 ± 704 ng/L, n = 42) classes of antibiotics were highest across all samples. ARGs were detected in all leachate samples with normalized sul2 and ermB levels being especially elevated (-1.37 ± 1.2 and -1.76 ± 1.6 log (copies/16S-rDNA), respectively). However, ARG abundances did not correlate with detected antibiotic levels, except for tetW and tetQ with TC levels (r = 0.88 and 0.81, respectively). In contrast, most measured ARGs did significantly correlate with heavy metal levels (p < 0.05), especially with Cd and Cr. This study shows high levels of ARGs and antibiotics can prevail in MSW leachates and landfills may be an underappreciated as a source of antibiotics and ARGs to the environment.

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Section: Resultsmentioning

confidence: 97%

Relationships between Antibiotics and Antibiotic Resistance Gene Levels in Municipal Solid Waste Leachates in Shanghai, China

Huang

Yang

et al. 2015

Environ. Sci. Technol.

267

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“…Exposure to high antibiotic concentrations (e.g., up to 100 mg/L) has been reported to select for antibiotic resistant plasmid-bearing cells, increase ARG copy numbers in resistant strains, and influence which ARGs are harbored within bacterial hosts . Conversely, the absence of the antibiotic (as well other chemical stressors for which ARGs may offer resistance, such as heavy metals) usually results in resistance plasmid curing (i.e., complete loss of the plasmid), possibly due to the metabolic burden of carrying an unneeded plasmid. , Nevertheless, curing is not always observed, and plasmid persistence has been shown to be enhanced by efficient replication and by partitioning and/or plasmid addiction systems. − …”

Section: Introductionmentioning

confidence: 99%

“…15 Conversely, the absence of the antibiotic (as well other chemical stressors for which ARGs may offer resistance, such as heavy metals) usually results in resistance plasmid curing (i.e., complete loss of the plasmid), possibly due to the metabolic burden of carrying an unneeded plasmid. 14,16 Nevertheless, curing is not always observed, and plasmid persistence has been shown to be enhanced by efficient replication and by partitioning and/or plasmid addiction systems. 17 Research on the effects of the growth medium and bacterial growth rate on plasmid stability has yielded different results that probably reflect differences in host/plasmid specific interactions.…”

Section: ■ Introductionmentioning

confidence: 99%

Tetracycline Resistance Gene Maintenance under Varying Bacterial Growth Rate, Substrate and Oxygen Availability, and Tetracycline Concentration

Rysz¹,

Mansfield²,

Fortner³

et al. 2013

Environ. Sci. Technol.

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Neither amplification nor attenuation of antibiotic resistance genes (ARG) in the environment are well understood processes. Here, we report on continuous culture and batch experiments to determine how tetracycline (TC), aerobic vs anaerobic conditions, bacterial growth rate, and medium richness affect the maintenance of plasmid-borne TC resistance (Tet(R)) genes. The response of E. coli (a model resistant strain excreted by farm animals) versus Pseudomonas aeruginosa (a model bacterium that could serve as a reservoir for ARGs in the environment) were compared to gain insight into response variability. Complete loss of the Tet(R) RP1 plasmid (56 kb) occurred for P. aeruginosa in the absence of TC, and faster loss was observed in continuous culture at higher growth rates. In contrast, E. coli retained its smaller pSC101 plasmid (9.3 kb) after 500 generations without TC (albeit at lower levels, with ratios of resistance to 16S rDNA genes decreasing by about 2-fold). A higher rate of ARG loss was observed in P. aeruginosa when grown in minimal growth medium (M9) than in richer Luria broth. Faster ARG loss occurred in E. coli under anaerobic (fermentative) conditions than under aerobic conditions. Thus, in these two model strains it was observed that conditions that ease the metabolic burden of plasmid reproduction (e.g., higher substrate and O2 availability) enhanced resistance plasmid maintenance; such conditions (in the presence of residual antibiotics) may be conducive to the establishment and preservation of ARG reservoirs in the environment. These results underscore the need to consider antibiotic concentrations, redox conditions, and substrate availability in efforts to evaluate ARG propagation and natural attenuation.

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“…Contrary to the observation in PBS without tetracycline, the inactivation rate of total E. coli showed no obvious difference compared with that of AR E. coli in the presence of tetracycline. These findings can be attributed to the selective pressure of antibiotics on the proliferation of ARB ( Rysz et al, 2013 ) as antibiotics exert selective pressure, which leads to the increase of the proportion of plasmid-bearing ARB ( Löser, 1995 ). Besides, the competitive light absorption effect between tetracycline and AR E. coli can also inhibit the photo-inactivation of AR E. coli .…”

Section: Photo-inactivation Of Ar E Colimentioning

confidence: 99%

Simulated sunlight-induced inactivation of tetracycline resistant bacteria and effects of dissolved organic matter

Zhang

Liu

et al. 2020

Water Research

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The transmission of antibiotic resistance in surface water has attracted much attention due to its increasing threat to human health. The role of sunlight irradiation and the effect of dissolved organic matter (DOM) on the transmission of antibiotic resistance are still unclear. In this study, photo-inactivation of antibiotic resistant bacteria (ARB) was investigated using antibiotic resistant E. coli (AR E. coli ) that contained the tetracycline resistance gene (Tc-ARG) as a representative. The results showed that AR E. coli underwent significant photo-inactivation due to the membrane damage induced by direct irradiation and by the generated reactive oxygen species. Simulated sunlight irradiation specifically suppressed the expression of tetracycline resistance, which is attributed to the destruction of tetracycline-specific efflux pump. Tetracycline inhibited the photo-inactivation of AR E. coli due to its selective pressure on tetracycline resistant E. coli and competitive light absorption effect. Suwannee River fulvic acid (SRFA), a representative DOM, promoted the inactivation of AR E. coli and further inhibited the expression of tetracycline resistance gene due to the generation of its excited triplet state, singlet oxygen, and hydroxyl radical. The extracellular Tc-ARG also underwent fast photodegradation under light irradiation and in the presence of SRFA, which leads to the decrease of its transformation efficiency. This study provided insight into the sunlight-induced inactivation of ARB, which is of significance for understanding the transmission of tetracycline resistance in surface water.

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Stability of the pBR322 plasmid derivative pBB210 in Escherichia coli TG1 under non‐selective and selective conditions

Cited by 5 publications

References 6 publications

Relationships between Antibiotics and Antibiotic Resistance Gene Levels in Municipal Solid Waste Leachates in Shanghai, China

Relationships between Antibiotics and Antibiotic Resistance Gene Levels in Municipal Solid Waste Leachates in Shanghai, China

Tetracycline Resistance Gene Maintenance under Varying Bacterial Growth Rate, Substrate and Oxygen Availability, and Tetracycline Concentration

Simulated sunlight-induced inactivation of tetracycline resistant bacteria and effects of dissolved organic matter

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