Stability of the intra- and extracellular toxins of Prymnesium parvum using a microalgal bioassay

Blossom, Hannah E.; Andersen, Niels Gerner; Rasmussen, Silas Anselm; Hansen, Per Juel

doi:10.1016/j.hal.2013.11.006

Cited by 33 publications

(26 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This could explain why different strains of P. parvum express different toxicities [9] and could be the reason for the discrepancy between the results reported in this study (strain: UTEX-2797) and those previously reported by Bergmann et al [41] for an unknown strain. Furthermore, observed P. parvum toxicity is sensitive to handling [44] and the composition of the aquatic environment [43]. Lastly, the material used in the experimental setup affects observed toxicity as well, as prymnesins readily bind to polymeric surfaces (log d = 3.4-5.2) [60].…”

Section: Discussionmentioning

confidence: 99%

“…Significant variations in toxicity have been reported when exposing fish externally to P. parvum culture [8,40]. The P. parvum toxicity has been reported to vary with pH [7,43], temperature, adsorbents, oxidizing agents [43], and mechanical disturbance [44]. All of the above contribute to our choice to normalize the exposure to culture cell count and regulate environmental factors such as temperature and oxygen levels.…”

Section: Algae Culture and Exposurementioning

confidence: 99%

See 1 more Smart Citation

Effects of Harmful Algal Blooms on Fish: Insights from Prymnesium parvum

Svendsen

Andersen

Hansen

et al. 2018

Fishes

View full text Add to dashboard Cite

Blooms of the planktonic alga Prymnesium parvum pose a global threat, causing fish kills worldwide. Early studies on the exposure of fish to P. parvum indicate that toxic effects are related to gill damage. The more strictly defined concept of adverse outcome pathways has been suggested as a replacement for the mode of action in toxicology studies. In this study, rainbow trout (Onchorhyncus mykiss) were exposed to P. parvum. During exposure, oxygen consumption was determined by respirometry, and ventilation and coughing rate were determined via video surveillance. Per breath oxygen consumption was calculated to assess the ventilation effort to obtain a unit of oxygen. A second experiment monitored fish behavior to assess recovery. The results indicated that oxygen consumption initially increased, but on average fell below the standard oxygen consumption at 70% relative exposure. Being a function of ventilation frequency and oxygen consumption, the per breath oxygen consumption decreased throughout exposure. Behavioral results determined that short-term P. parvum exposure subsequently caused the exposed fish to seek flow refuge immediately and to a greater extent than unexposed fish. The adverse outcome pathway of P. parvum on rainbow trout is that P. parvum acts as a gill irritant resulting in non-recoverable respiratory failure.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Algae Culture and Exposurementioning

confidence: 99%

Effects of Harmful Algal Blooms on Fish: Insights from Prymnesium parvum

Svendsen

Andersen

Hansen

et al. 2018

Fishes

View full text Add to dashboard Cite

show abstract

“…A recent study has reported the development of another bioassay for P. parvum's toxins involving lysis of the cryptophyte Teleaulax acuta as measured by the decrease in its relative fluorescence (Blossom et al, 2014a). Using that assay, they also found that the intracellular toxins released by sonication were at lower levels than the extracellular toxins.…”

Section: Discussionmentioning

confidence: 99%

“…Using that assay, they also found that the intracellular toxins released by sonication were at lower levels than the extracellular toxins. However, they found the latter to be extremely unstable after only 24 h at 4 C, and after 3 days of storage at À20 C (Blossom et al, 2014a). Further studies by Blossom et al (2014b) have indicated that ichthyotoxicity and T. acuta lysis are not correlated, suggesting that the Teleaulax assay does not measure ichthyotoxicity due to PPs.…”

Section: Discussionmentioning

confidence: 99%

Semi-quantitative assay for polyketide prymnesins isolated from Prymnesium parvum (Haptophyta) cultures

Claire

Manning

Talarski

2015

Toxicon

View full text Add to dashboard Cite

“…One possible explanation is related to the stability of potential allelochemicals. A recent study of the lytic activity of Prymnesium parvum has demonstrated that the extracellular toxins in the supernatant are highly unstable, and the lytic activity of the intracellular toxins, when released by sonication, is not as high as that of the extracellular toxins (Blossom et al 2014). Similarly, the extracellular allelochemicals of Pseudo-nitzschia spp.…”

Section: Characteristics Of the Allelopathy Of Pseudo-nitzschia Sppmentioning

confidence: 99%

Ability of the marine diatoms Pseudo-nitzschia multiseries and P. pungens to inhibit the growth of co-occurring phytoplankton via allelopathy

Xu¹,

Tang²,

Qin³

et al. 2015

Aquat. Microb. Ecol.

View full text Add to dashboard Cite

Diatoms within the genus Pseudo-nitzschia can form near-monospecific blooms in both natural and iron-fertilized high-nutrient, low-chlorophyll (HNLC) regions and can have detrimental impacts on marine ecosystems. Here, we demonstrate the ability of P. pungens isolated from the South China Sea and 2 strains of P. multiseries isolated from the Bay of Fundy, Canada, to produce extracellular compounds capable of lysing and/or inhibiting the growth of multiple phytoplankton species. Since the allelopathic activity was found in both P. multiseries, which produces domoic acid (DA), and P. pungens, which produces little if any DA, the allelopathic effects of Pseudo-nitzschia spp. seem to be unrelated to this compound. Allelopathic inhibition of other phytoplankton was documented during exponential and stationary growth phases of Pseudonitzschia, and the strongest allelopathic effects were obtained from sonicated cultures, suggesting that the sudden release of allelochemicals via processes such as cell lysis or zooplankton grazing may have the strongest effect in an ecosystem setting. Differences in the responses of target species to Pseudo-nitzschia spp. suggest these algae may produce multiple compounds that vary in their allelopathic potency and composition as a function of species, strain, growth stage, and perhaps other factors. Collectively, these results suggest that the allelopathy may affect competition between Pseudo-nitzschia spp. and other phytoplankton and may play an important role in the formation and persistence of natural and iron-fertilized blooms.KEY WORDS: Allelopathy · Pseudo-nitzschia multiseries · Pseudo-nitzschia pungens · Harmful algal bloom (HAB) · Competition · Phytoplankton Resale or republication not permitted without written consent of the publisher

show abstract

Stability of the intra- and extracellular toxins of Prymnesium parvum using a microalgal bioassay

Cited by 33 publications

References 37 publications

Effects of Harmful Algal Blooms on Fish: Insights from Prymnesium parvum

Effects of Harmful Algal Blooms on Fish: Insights from Prymnesium parvum

Semi-quantitative assay for polyketide prymnesins isolated from Prymnesium parvum (Haptophyta) cultures

Ability of the marine diatoms Pseudo-nitzschia multiseries and P. pungens to inhibit the growth of co-occurring phytoplankton via allelopathy

Contact Info

Product

Resources

About