Stability of the Hypoosmotic Swelling Test Over Time

Shanis, Bonnie S.; Check, Jerome H.; Bollendorf, A.; Lurie, Deborah

doi:10.3109/01485019208987734

Cited by 10 publications

(13 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hypoosmotic swelling (HOS) test is a simple, easily reproducible test that evaluates the functional integrity of the sperm membrane [ 161. In contrast to sperm count and percent motility, this test is very stable over time [24]. When less than 50% of the sperm show characteristic swelling of sperm tails there is a correlation with very poor PRs, even when all other semen parameters are normal [lo].…”

mentioning

confidence: 99%

Comparison of Direct Swim-Up, Mini-Percoll, and Sephadex G10 Separation Procedures

Bollendorf

Check

Katsoff

et al. 1994

Archives of Andrology

Self Cite

View full text Add to dashboard Cite

A comparison was made of three sperm separation techniques--direct swim-up, mini-Percoll, and Sephadex G-10--on semen parameters, including count, % motility, forward progression, and hypoosmotic swelling (HOS) test scores. Overall, the best quality sperm (less debris, best % and quality motility, and best HOS scores) were noted with the direct swim-up procedure. Mini-Percoll resulted in the highest count, but the worst HOS score. Sephadex G-10 resulted in quality of semen specimen almost as good as the direct swim-up procedure, but did allow some noncellular debris and bacteria to filter through the column.

show abstract

mentioning

confidence: 99%

Comparison of Direct Swim-Up, Mini-Percoll, and Sephadex G10 Separation Procedures

Bollendorf

Check

Katsoff

et al. 1994

Archives of Andrology

Self Cite

View full text Add to dashboard Cite

show abstract

“…The HOS test is easy to perform and not expensive; in contrast to sperm count and motility, which frequently fluctuate, the HOS test is stable over time (Shanis et al, 1992). However, at the moment, the HOS test is not included in standard semen analyses and is rarely considered in the workup of unexplained infertility.…”

Section: Discussionmentioning

confidence: 99%

Males With Subnormal Hypo‐Osmotic Swelling Test Scores Have Lower Pregnancy Rates Than Those With Normal Scores When Ovulation Induction and Timed Intercourse Is Used as a Treatment for Mild Problems With Sperm Count, Motility, or Morphology

Tartagni¹,

Cicinelli²,

Schonauer³

et al. 2004

Journal of Andrology

View full text Add to dashboard Cite

This study was designed to evaluate the effectiveness and clinical usefulness of the hypo-osmotic swelling (HOS) test in predicting successful conception in couples in which men with mild male-factor infertility criteria were undergoing a timed vaginal inter-course protocol. One hundred couples, in which mild male infertility was the only abnormality, were included in the study. Semen was analyzed according to standard World Health Organization (WHO) criteria and subjected to the HOS test. Patients were divided into 2 groups: group 1 (n=39) with normal HOS test and group 2 (n=61) with abnormal HOS test. All women underwent three consecutive cycles of follicular growth ultrasound monitoring and timed intercourse. Ten couples were exclude from the study. Ten clinical pregnancies were achieved in group 1 with a pregnancy rate per patient and per cycle of 28.5% and 9.5%, respectively. In group 2, 6 pregnancies were achieved, with a pregnancy rate per patient and per cycle of 10.9% and 3.6%, respectively. Both pregnancy rates per patients and per cycle was significantly higher (P <.05) in group 1 than in group 2. The HOS test may be considered an easy and reliable test in identifying among subfertile men those who have a greater possibility of causing pregnancy.

show abstract

“…The toxic factor may be proteinaceou s in nature since, anecdotally , treatment of the sperm with the protein digestive enzyme chymotrypsin frequently brings the subnormal HOST score to above 50% [53]. Thus, the HOST when subnormal correlates much better with subfertility than either motility density or morphology [56,75].…”

Section: Hyp O Os M Otic S W El Ling Tes T (Ho S T)mentioning

confidence: 93%

Prognosis for Sperm Fertilizability: Analysis of Different Variables in Men

Check

Katsoff

2002

Archives of Andrology

Self Cite

View full text Add to dashboard Cite

An overview of various sperm tests is presented. The standard semen analysis obtained by most clinicians evaluating infertility usually consists of sperm concentration, percent motility, quality of motility, and sperm morphology. Unfortunately, unless the motile density is extremely low, the count and motility are not good prognosticators of fertility potential. Values above the norm for normal fertile couples unfortunately cannot reliably predict normal fertility potential. It is important to find sperm tests that are easy to perform, are relatively inexpensive, and provide an accurate prognosis. Strict morphology was hoped to be such a tool with initial optimism that it was far superior to standard morphology. Unfortunately, this test also failed to be the ideal inexpensive prognostic test after further evaluation. One test that is inexpensive and highly correlates with fertilizability is the presence of antisperm antibodies since their presence frequently does not alter count, motility, or morphology. This test should be performed as part of the routine semen analysis. Other tests highly correlate with the achievement of pregnancy and are simple and inexpensive to perform, but, interestingly, do not correlate with fertilizability. These include the hypoosmotic swelling test (HOST) and the sperm stress test. Abnormalities in these tests imply a different abnormality of sperm that leads to conception failure and that is the transfer of a toxic factor from the sperm to oocyte to embryo that prevents the embryo from implanting. Certainly, the simple, inexpensive HOST should be performed routinely. Other tests of sperm function, e.g., sperm penetration assay, sperm zona pellucida binding assay, and acrosome reaction, have their definite place in the evaluation of the infertile male. However, because they are expensive and difficulty to perform they lend themselves to certain specific circumstances but not to routine testing.

show abstract

Stability of the Hypoosmotic Swelling Test Over Time

Cited by 10 publications

References 6 publications

Comparison of Direct Swim-Up, Mini-Percoll, and Sephadex G10 Separation Procedures

Comparison of Direct Swim-Up, Mini-Percoll, and Sephadex G10 Separation Procedures

Males With Subnormal Hypo‐Osmotic Swelling Test Scores Have Lower Pregnancy Rates Than Those With Normal Scores When Ovulation Induction and Timed Intercourse Is Used as a Treatment for Mild Problems With Sperm Count, Motility, or Morphology

Prognosis for Sperm Fertilizability: Analysis of Different Variables in Men

Contact Info

Product

Resources

About