Stability of the Cobalamin Moiety in Buffered Aqueous Solutions of Hydroxocobalamin

Marcus, Arnold D.; Stanley, Josephine L.

doi:10.1002/jps.2600530118

Cited by 13 publications

(4 citation statements)

References 6 publications

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“…Therefore, the assay data on the degradation of B 12 and B 12b were subjected to kinetic treatment, and it was found that these vitamins degrade by an apparent first-order kinetics in the presence of AH 2 . This is in agreement with the previous observations that the chemical degradation of B 12 and B 12b follows first-order kinetics (34)(35)(36). The apparent first-order rate constants (k obs ) for the degradation of B 12 and B 12b are given in Table III (28,46), but no comparison of the kinetic data in these reactions has been made.…”

Section: Kinetics Of Degradation Of B 12 and B 12bsupporting

confidence: 89%

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Effect of Ascorbic Acid on the Degradation of Cyanocobalamin and Hydroxocobalamin in Aqueous Solution: A Kinetic Study

et al. 2014

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Abstract. The degradation kinetics of 5×10−5 M cyanocobalamin (B 12 ) and hydroxocobalamin (B 12b ) in the presence of ascorbic acid (AH 2 ) was studied in the pH range of 1. , respectively, indicating a greater effect of AH 2 on B 12b compared to that of B 12 . The k obs -pH profiles for both B 12 and B 12b show the highest rates of degradation around pH 5. The degradation of B 12 and B 12b by AH 2 is affected by the catalytic effect of phosphate ions on the oxidation of AH 2 in the pH range 6.0-8.0.

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Section: Kinetics Of Degradation Of B 12 and B 12bsupporting

confidence: 89%

“…3,3-Dimethyl-2,5-dioxopyrrolidine-4-propionamide and 3,3-dimethyl-2,5-dioxopyrrolidine-4-propionic acid have been identified as the oxidation products of B 12 (33). The chemical (34)(35)(36) and photodegradation (21,37) of B 12 and B 12b has been reported to follow first-order kinetics.…”

Section: Introductionmentioning

confidence: 99%

Effect of Ascorbic Acid on the Degradation of Cyanocobalamin and Hydroxocobalamin in Aqueous Solution: A Kinetic Study

et al. 2014

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“…Radioactive CN[ 57 Co]Cbl was converted into HO[ 57 Co]Cbl by photoaquation in an acidic medium under nitrogen bubbling as previously described [ 4 ]. The purity of the conversion product was analysed by HPLC employing an in-house method [ 5 ] and found to be >95% both at the time of production (data not shown) and after storage under conditions, known to ensure stability of the product (pH 6.0 at 4 °C) [ 6 ] for 5 months (Fig. 2 ).…”

Section: Methodsmentioning

confidence: 99%

Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats

et al. 2017

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PurposeHydroxocobalamin (HOCbl) is the dominating Cbl form in food, whereas cyanocobalamin (CNCbl) is common in vitamin pills and oral supplements. This study compares single-dose absorption and distribution of oral HO[57Co]Cbl and CN[57Co]Cbl in Cbl-deficient and normal rats.MethodsMale Wistar rats (7 weeks) were fed a 14-day diet with (n = 15) or without (n = 15) Cbl. We compared the uptakes of HO[57Co]Cbl (free or bound to bovine transcobalamin) and free CN[57Co]Cbl administered by gastric gavage (n = 5 in each diet group). Rats were sacrificed after 24 h. Blood, liver, kidney, brain, heart, spleen, intestines, skeletal muscle, 24-h urine and faeces were collected, and the content of [57Co]Cbl was measured. Endogenous Cbl in tissues and plasma was analysed by routine methods.ResultsMean endogenous plasma-Cbl was sevenfold lower in deficient vs. normal rats (190 vs. 1330 pmol/L, p < 0.0001). Cbl depletion increased endogenous Cbl ratios (tissue/plasma = k in/k out) in all organs except for the kidney, where the ratio decreased considerably. Twenty-four-hour accumulation of labelled Cbl showed that HOCbl > CNCbl (liver) and CNCbl > HOCbl (brain, muscle and plasma).ConclusionsThe Cbl status of rats and the administered Cbl form influence 24-h Cbl accumulation in tissues and plasma.Electronic supplementary materialThe online version of this article (doi:10.1007/s00394-017-1424-0) contains supplementary material, which is available to authorized users.

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“…Examples of this are hydrolysis of the following compounds in aqueous solution: streptozotocin (12), streptovaricin (1 3), diethylaminoethylsalicylate hydrochloride (14), acetylsalicylic acid anhydride (19, procaine hydrochloride (16, 17), chloramphenicol (18), N-acetyl-p-aminophenol (19), chlorobutanol (20), ascorbic acid (21, 22), phenobarbital (23), idoxuridine (24), penicillin at low pH (25), morphine (26), cycloserine (27), and hydroxocobalamin (28).…”

Section: Examplesmentioning

confidence: 99%

Kinetic Salt Effect in Pharmaceutical Investigations

Carstensen

1970

Journal of Pharmaceutical Sciences

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Stability of the Cobalamin Moiety in Buffered Aqueous Solutions of Hydroxocobalamin

Cited by 13 publications

References 6 publications

Effect of Ascorbic Acid on the Degradation of Cyanocobalamin and Hydroxocobalamin in Aqueous Solution: A Kinetic Study

Effect of Ascorbic Acid on the Degradation of Cyanocobalamin and Hydroxocobalamin in Aqueous Solution: A Kinetic Study

Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats

Kinetic Salt Effect in Pharmaceutical Investigations

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