Stability of rice bran oil extracted by SFE and soxhlet methods during accelerated shelf-life storage

Abstract

SUMMARY:Supercritical fluid high oryzanol (SFE HO) and supercritical fluid low oryzanol (SFE LO) rice bran oils were obtained and compared with that extracted by the Soxhlet (SOX) method. Their composition and stability during storage were determined. The amount of unsaponifiable matter and gamma oryzanol of SFE samples were significantly (p<0.05) higher than SOX. While the amount of tocopherol in SOX (4.0 mg·g -1 ) was higher than that reported in SFE HO and SFE LO, at 3.2 and 2.6 mg·g -1 , respectively. After storage for 42 days at 70 °C the PV, FFA%, conjugated diene and p-anisidine values of SOX were higher than those of SFE HO, and SFE LO. The SFE samples showed better stability than SOX under successive heating and the addition of BHA decreased PV, FFA%, conjugated diene and p-anisidine levels in all samples. KEYWORDS:Oryzanol; Rice bran oil; Shelf life; Soxhlet; Stability; Supercritical fluid extraction; Tocopherol RESUMEN: Estabilidad de aceites de salvado de arroz obtenidos mediante SFE y Soxhlet durante su vida útil en almacenamiento acelerado. Se realizaron extracciones mediante fluido supercrítico de salvado de arroz con alto (SFE HO) y bajo (SFE LO) contenido en orizanol y se comparó con los extraídos mediante Soxhlet (SOX). Se determinó la composición y la estabilidad durante el almacenamiento. La materia insaponificable y el gamma orizanol de las muestras SFE fueron significativamente más altas (p<0,05) que SOX. Mientras la cantidad de tocoferol en SOX (4,0 mg·g -1 ) fue mayor que lo encontrado para SFE HO y SFE LO, 3,2 y 2,6 mg·g -1 respectivamente. Después de 42 días de almacenamiento a 70 °C los valores de PV, FFA %, dienos conjugados y p-anisidina de SOX fueron mayores que los de SFE HO, y SFE LO. Las muestras SFE mostraron mejor estabilidad que SOX bajo calentamiento y la adición de BHA disminuyó el PV, FFA %, dienos conjugados y los niveles de p-anisidina en todas las muestras.

Comparative Studies between Conventional and Microwave Assisted Extraction for Rice Bran Oil

“…In Case of solvent extraction different food grade solvents can be used like hexane, acetone, isopropanol, ethanol etc. 4,6 . Microwave assisted extraction and Ultrasonication is the advanced methodology to extract oil and it is a green process for oil extraction 7 9 .…”

Supercritical CO₂ Extraction of Rice Bran Oil –the Technology, Manufacture, and Applications

“…After storage for 42 days at 70 , the peroxide value, free fatty acid percentage, conjugated diene and panisidine values of the Soxhlet extracted RBO were higher than those of SC-CO 2 extraction. Thus, the SC-CO 2 RBO samples showed better stability than those RBO samples obtained from Soxhlet extraction 53 . Besides its stability in terms of edible oil, the physiological safety of SC-CO 2 extracted RBO has also been evaluated.…”

“…The RBO phytochemical contents under the optimum conditions of 50 MPa, 60 for 90 min were up to 1,800 ppm of tocols, 19,000 ppm of sterols, and 11,000 ppm of oryzanols 52 . On the other hand, the stabilities of RBOs obtained by SC-CO 2 extraction and hexane Soxhlet method were studied during accelerated shelf-life storage 53 . Compositionally, unsaponifiable matters and γ-oryzanols of SC-CO 2 samples were higher than from hexane Soxhlet extraction, but the amount of tocopherol was lower.…”

Extraction and Characterization of Oil from Mudong bayberry (Myrica rubra) Kernels

“…Briefly, the powder of bayberry kernels (5.00 g) was placed in a Soxhlet apparatus and then continuously extracted for 8 h using aether (34.6°C). After extraction, the aether was removed at 45.0°C under reduced pressure with a rotary vacuum evaporator (Mariod et al, 2015). The oil was then dried in an oven at 65.0°C to remove residual solvent and water.…”