Stability of plasma analytes after delayed separation of whole blood: implications for epidemiological studies

Clark, Sarah L.; Youngman, Linda; Palmer, Alison; Parish, Sarah; Pető, Richárd; Collins, Rory

doi:10.1093/ije/dyg023

Cited by 123 publications

(91 citation statements)

References 6 publications

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“…The short-term storage (4 years) revealed non-significant differences for most components. Although the Janus samples were exposed for prolonged clot-time at 4°C for up to 28 h, studies have reported that a similar sample handling had small or negligible impact on cholesterol and lipoproteins [9][10][11][12][13], and the hormones LH, PRL, FSH and FT4 [12,[14][15][16].…”

Section: Discussionmentioning

confidence: 99%

Stability of selected serum hormones and lipids after long-term storage in the Janus Serum Bank

Gislefoss

Grimsrud

Mørkrid

2015

Clinical Biochemistry

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Section: Discussionmentioning

confidence: 99%

Stability of selected serum hormones and lipids after long-term storage in the Janus Serum Bank

Gislefoss

Grimsrud

Mørkrid

2015

Clinical Biochemistry

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“…Some analytical issues related to nutritional biomarker measurements were previously discussed in a series of papers (Hankinson et al, 1989;Key et al, 1996;Comstock et al, 2001;Clark et al, 2003;Clark et al, 2004). These papers focused on analytical issues related to analysis of only a few markers in whole blood or sera samples.…”

Section: Introductionmentioning

confidence: 99%

Relative contribution of specific sources of systematic errors and analytical imprecision to metabolite analysis by HPLC–ECD

et al. 2005

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Objective interpretation of metabolomics data requires understanding both analytical and biological measurement errors. Here we address analytical measurement errors, the sources of these errors, and how this variability can impact metabolomic profiles. Sources considered include room temperature exposure (which could affect sample stability), spiking with authentic standards, the number of study replicates, the overall temporal design of the experimental series, and the complexity of the biological matrix of the samples (individual or pooled sera). The study focused on the analysis of $80 rat sera metabolites by HPLC coupled with coulometric array detectors. Time delay and room temperature exposure had minimal effects on the total relative metabolite concentrations and variability (mean: $94-98% of control, CV median : ±5-7%), but the concentrations of some specific metabolites were significantly altered. Changes observed in the concentrations of specific metabolites ranged as high as ±7-fold, with changes in variability ranging from 0.3% to 68%. Spiked samples demonstrated more complex behavior when allowed to decay over time than did control samples. The spiking of sera and standards with 43 known metabolites increased variability of the apparent concentrations of metabolites up to $24% as opposed to $3% in pure sera. Increased variability was metabolite-specific. In both pure and spiked sera, $80-95% of metabolites had CVs equivalent to standard analytical CVs for these metabolites. Experimental design, number of replicates, and complexity of the biological matrix had comparable effects. These results suggest that, under carefully controlled conditions, these analytical issues are not significant sources of variability relative to biological variation for most metabolites.

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“…Specification of storage modality of samples analyzed is very important since it is known that creatine, over time and changing pH of medium, varies its concentration transforming itself into creatinine [15,17]. Our plasma creatine and GAA reference values are determined by large group of control subject allowing us to obtain three age-related ranges, clearly indicating storage modality of samples.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous determination of creatine and guanidinoacetate in plasma by liquid chromatography–tandem mass spectrometry (LC–MS/MS)

Boenzi

Rizzo

Ciommo

et al. 2011

Journal of Pharmaceutical and Biomedical Analysis

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a b s t r a c tBackground: Guanidinoacetate (GAA) and creatine are reliable biochemical markers for primary and secondary creatine defects. We describe a method by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) for simultaneous determination of plasma GAA and creatine. We analyzed 283 healthy subjects from 0 to 63 years old to obtain age-related control values. Methods: Plasma samples were extracted with acetonitrile containing 13C2-GAA and d3-creatine. Samples were analyzed by LC-MS/MS in positive ionisation mode, after derivatization to butyl-esters. Optimal chromatographic separation was achieved using a column Supelcosil TM LC-4.6 mm with isocratic elution in 5 min. Results: Run time was 5 min. Standard curves were linear from 0.05 to 200 mol/L for creatine and from 0.02 to 40 mol/L for GAA. Limit of detection (LOD) and limit of quantitation (LOQ) were respectively 0.005 and 0.05 mol/L for creatine; LOD and LOQ were 0.002 and 0.02 mol/L respectively for GAA. Intra and inter-assay CVs for creatine and GAA were <8%. Recovery experiments adding 50 and 100 mol/L creatine and 10 and 20 mol/L GAA were 102.1% and 101.2%, for creatine; 102.95% and 96.45% for GAA. The method was applied to 283 plasma controls from healthy subjects to obtain control values in three specific age ranges: 0-12, 13-20, >20 years old. Conclusion: A rapid and high sensitive LC-MS/MS method was developed and validated for determination of creatine and GAA in plasma and it could also be applied to other biological materials, such as CFS and urines. This method is useful for diagnoses of primary and also for secondary creatine defects that may occur in inherited metabolic diseases in which precursors of creatine biosynthesis are involved.

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Stability of plasma analytes after delayed separation of whole blood: implications for epidemiological studies

Abstract: A wide range of important analytes, including lipids, change by only a few per cent in whole blood during storage at room temperature for several days. Mailed transport of whole blood samples may, therefore, be a simple and cost-effective option for large-scale epidemiological studies.

Cited by 123 publications

References 6 publications

Stability of selected serum hormones and lipids after long-term storage in the Janus Serum Bank

Stability of selected serum hormones and lipids after long-term storage in the Janus Serum Bank

Relative contribution of specific sources of systematic errors and analytical imprecision to metabolite analysis by HPLC–ECD

Simultaneous determination of creatine and guanidinoacetate in plasma by liquid chromatography–tandem mass spectrometry (LC–MS/MS)

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