Stability of phospholipid vesicles studied by asymmetrical flow field-flow fractionation and capillary electrophoresis

Yohannes, Gebrenegus; Pystynen, Kati-Henna; Riekkola, Marja‐Liisa; Wiedmer, Susanne Κ.

doi:10.1016/j.aca.2005.12.042

Cited by 38 publications

(21 citation statements)

References 48 publications

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“…Overall, in the absence of nisin, PG-containing vesicles were of greater size then PC liposomes, a phenomenon reported elsewhere. 14,42 This difference in size of PC liposomes vs those with PG incorporated is not thought to be a function of the zwitterion not being able to maintain stability because of charge repulsion at the surface, as the lipid has only a small electronegative surface charge at neutral pH (Table 1). With inclusion of nisin, the diameters of PG-containing liposomes decreased, whereas size of pure PC liposomes increased compared to their nisin-free counterparts ( Figure 2).…”

Section: Discussionmentioning

confidence: 96%

Characterization of Antimicrobial-bearing Liposomes by ζ-Potential, Vesicle Size, and Encapsulation Efficiency

et al. 2007

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Liposome entrapment may improve activity of protein or polypeptide antimicrobials against a variety of microorganisms. In this study, ability of liposomes to withstand exposure to environmental and chemical stresses typically encountered in foods and food processing operations were tested. Liposomes consisting of distearoylphosphatidylcholine (PC) and distearoylphosphatidylglycerol (PG), with 0, 5, or 10 μg/ml of the antimicrobial peptide nisin entrapped, were exposed to elevated temperatures (25-75°C) and a range of pH (5.5-11.0). Ability of liposomes to maintain integrity was assessed by measuring the encapsulation efficiency (EE), z-potential, and particle size distribution of liposomes. Distearoylphosphatidylcholine, PC/PG 8:2, and PC/PG 6:4 (mole fraction) liposomes retained between~70-90% EE despite exposure to elevated temperature and alkaline or acidic pH. Particle size of liposomes averaged between 100 and 240 nm depending on liposome preparation. Liposomal surface charge depended primarily on phospholipid composition and changed little with inclusion of nisin. Surface charge was not affected by temperature for PC and PC/PG 8:2 but decreased for PC/PG 6:4 liposomes. Our results suggest that liposomes containing nisin may be suitable for use as antimicrobial-active ingredients in low-or high-pH foods subjected to moderate heat treatments.

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Section: Discussionmentioning

confidence: 96%

Characterization of Antimicrobial-bearing Liposomes by ζ-Potential, Vesicle Size, and Encapsulation Efficiency

et al. 2007

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“…At 25 ± 2 C/60 ± 5% RH and 40 ± 2 C/75 ± 5% RH, an increase in the average vesicular size and percent transmittance was observed. After 2 weeks of storage at 40 ± 2 C/75 ± 5% RH, the visible signs of physical instability like vesicle fusion, aggregation, disruption and sedimentation were observed due to the fusion of phospholipid membrane of the ethosomes [35]. After 12 weeks of storage, substantial amount of drug leakage (11.86 ± 0.13%) was observed at 40 ± 2 C/75 ± 5% RH.…”

Section: Stability Studiesmentioning

confidence: 99%

Nanosized ethosomes-based hydrogel formulations of methoxsalen for enhanced topical delivery against vitiligo: formulation optimization, in vitro evaluation and preclinical assessment

Garg

Beg

et al. 2015

Journal of Drug Targeting

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The present investigation aimed for the development and characterization of ethosomes-based hydrogel formulations of methoxsalen for enhanced topical delivery and effective treatment against vitiligo. The ethosomes were prepared by central composite design (CCD) and characterized for various quality attributes like vesicle shape, size, zeta potential, lamellarity, drug entrapment and drug leaching. The optimized ethosomes were subsequently incorporated int Carbopol® 934 gel and characterized for drug content, rheological behavior, texture profile, in vitro release, ex vivo skin permeation and retention, skin photosensitization and histopathological examination. Ethosomes were found to be spherical and multilamellar in structures having nanometric size range with narrow size distribution, and high encapsulation efficiency. Ethosomal formulations showed significant skin permeation and accumulation in the epidermal and dermal layers. The fluorescence microscopy study using 123 Rhodamine exhibited enhanced permeation of the drug-loaded ethosomes in the deeper layers of skin. Also, the developed formulation showed insignificant phototoxicity and erythema vis-à-vis the conventional cream. The results were cross-validated using histopathological examination of skin segments. In a nutshell, the ethosomes-based hydrogel formulation was found to be a promising drug delivery system demonstrating enhanced percutaneous penetration of methoxsalen with reduced phototoxicity and erythema, thus leading to improved patient compliance for the treatment against vitiligo.

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“…Increases in liposome particle size over time have been observed due to agglomeration and fusion of liposomes during storage (Fan et al 2007). However, in other studies liposomes remained stable in terms of particle size for at least 5 months when stored at 4 C (Yohannes et al 2006, Garg et al 2007. Liposome composition appears to be a key factor, with the inclusion of negatively charged phospholipids, such as DOPS, thought to reduce the tendency for aggregation and particle size increases and reduce the rate of drug leakage during storage (Manosroi et al 2002).…”

Section: Delivery Of Rslpi In a Liposomal Carrier For Inhalation 519mentioning

confidence: 99%

Delivery of rSLPI in a liposomal carrier for inhalation provides protection against cathepsin L degradation

Gibbons

McElvaney

Taggart

et al. 2009

Journal of Microencapsulation

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Secretory leukocyte protease inhibitor (SLPI) is an endogenous serine protease inhibitor that protects the lungs from excessive tissue damage caused by leukocyte proteases released during inflammation. Recombinant SLPI (rSLPI) has shown potential as a treatment for inflammatory lung conditions. To date, its clinical application has been limited by rapid enzymatic cleavage by cathepsins and rapid clearance from the lungs after inhalation. In this study, rSLPI was encapsulated in 1,2-Dioleoylsn-Glycero-3-[Phospho-L-Serine] : Cholesterol (DOPS : Chol) liposomes for inhalation. Incubation of rSLPI with cathepsin L leads to complete loss of activity while encapsulation of rSLPI in DOPS : Chol liposomes retained 92.6% of its activity after challenge with cathepsin L. rSLPI-loaded liposomes were aerosolized efficiently using a standard nebulizer with a minimal loss of activity and stability. This formulation was biocompatible and encapsulation did not appear to diminish access to intracellular sites of action in in vitro cell culture studies. Liposome encapsulation of rSLPI therefore improves stability and potentially reduces the level and frequency of dosing required for therapeutic effect after inhalation.

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Stability of phospholipid vesicles studied by asymmetrical flow field-flow fractionation and capillary electrophoresis

Cited by 38 publications

References 48 publications

Characterization of Antimicrobial-bearing Liposomes by ζ-Potential, Vesicle Size, and Encapsulation Efficiency

Characterization of Antimicrobial-bearing Liposomes by ζ-Potential, Vesicle Size, and Encapsulation Efficiency

Nanosized ethosomes-based hydrogel formulations of methoxsalen for enhanced topical delivery against vitiligo: formulation optimization, in vitro evaluation and preclinical assessment

Delivery of rSLPI in a liposomal carrier for inhalation provides protection against cathepsin L degradation

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