Stability of neural differentiation in human adipose derived stem cells by two induction protocols

Ahmadi, Nafiseh; Razavi, Shahnaz; Kazemi, Mohammad; Oryan, Shahrbanoo

doi:10.1016/j.tice.2011.11.006

Cited by 56 publications

(50 citation statements)

References 62 publications

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“…Some studies have reported that when ADSCs are cultured in NSC medium, they can form NSClike neural spheres and even could further differentiate into neural cells (Ahmadi et al, 2012;Anghileri et al, 2008). However, there was a question of whether these cells are really neurons because these investigators did not determine whether these NSC-like neural spheres express the NSCspecific markers Pax6 and Sox2.…”

Section: Discussionmentioning

confidence: 99%

Conversion of Adipose Tissue-Derived Mesenchymal Stem Cells to Neural Stem Cell-Like Cells by a Single Transcription Factor, Sox2

Qin

Zhou

Wang

et al. 2015

Cellular Reprogramming

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Adipose tissue is an attractive source of easily accessible adult candidate cells for regenerative medicine. Adipose tissue-derived mesenchymal stem cells (ADSCs) have multipotency and strong proliferation and differentiation capabilities in vitro. However, as mesodermal multipotent stem cells, whether the ADSCs can convert into induced neural stem cells (NSCs) has so far not been demonstrated. In this study, we found that normally the naïve ADSCs cultured as either monolayer or spheres in NSC medium did not express Sox2 and Pax6 genes and proteins, and could not differentiate to neuron-like cells. However, when we introduced the Sox2 gene into ADSCs by retrovirus, they exhibited a typical NSC-like morphology, and could be passaged continuously, and expressed NSC specific markers Sox2 and Pax6. In addition, the ADSC-derived NSC-like cells displayed the ability to differentiate into neuron-like cells when switched to the differentiation culture medium, expressing neuronal markers, including Tuj1 and MAP2 genes and proteins. Our results suggest the ADSCs can be converted into induced NSC-like cells with a single transcription factor Sox2. This finding could provide another alternative cell source for cell therapy of neurological disorders.

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Section: Discussionmentioning

confidence: 99%

Conversion of Adipose Tissue-Derived Mesenchymal Stem Cells to Neural Stem Cell-Like Cells by a Single Transcription Factor, Sox2

Qin

Zhou

Wang

et al. 2015

Cellular Reprogramming

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“…Hsueh et al [96] observed that, when seeded on a chitosan-coated surface, human ASCs form spheres containing up to 19.5% nestin positive cells. Ahmadi et al [97] further reported that, after culture in serum-free medium, 51% nestin-positive cells could be generated from human ASCs. However, Zuk et al [43] found that nestin was expressed in multiple cell types, including myogenic, endothelial, and hepatic cells, indicating that nestin expression alone is not suitable for the identification of neural cells, especially without functional analysis.…”

Section: Neurogenic and Other Lineagesmentioning

confidence: 99%

Dedifferentiated fat cells: A cell source for regenerative medicine

Jumabay¹,

Boström²

2015

WJSC

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The identification of an ideal cell source for tissue regeneration remains a challenge in the stem cell field. The ability of progeny cells to differentiate into other cell types is important for the processes of tissue reconstruction and tissue engineering and has clinical, biochemical or molecular implications. The adaptation of stem cells from adipose tissue for use in regenerative medicine has created a new role for adipocytes. Mature adipocytes can easily be isolated from adipose cell suspensions and allowed to dedifferentiate into lipidfree multipotent cells, referred to as dedifferentiated fat (DFAT) cells. Compared to other adult stem cells, the DFAT cells have unique advantages in their abundance, ease of isolation and homogeneity. Under proper condition in vitro and in vivo , the DFAT cells have exhibited adipogenic, osteogenic, chondrogenic, cardiomyogenc, angiogenic, myogenic, and neurogenic potentials. In this review, we first discuss the phenomena of dedifferentiation and transdifferentiation of cells, and then dedifferentiation of adipocytes in particular. Understanding the dedifferentiation process itself may contribute to our knowledge of normal growth processes, as well as mechanisms of disease. Second, we highlight new developments in DFAT cell culture and summarize the current understanding of DFAT cell properties. The unique features of DFAT cells are promising for clinical applications such as tissue regeneration.

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“…In addition, ADSCs possess additional characteristics including immunomodulatory properties that can alter the cytokine secretion profile of immune cells and act as source of variety of growth factors. In according to our previous study, in vitro differentiation of ADSCS into neuron cells is achieved by culture with media containing selective lineage specific induction factors [21].…”

Section: Review Transdifferentiation Potential Of Adscs Into Neuron Cmentioning

confidence: 99%

“…ADSCs when cultured in the appropriate conditions are capable to differentiate into other cells outside their lineage such as neuronal, glial and neurotrophic factors secreting cells (NTFs) [14][15][16][19][20][21][22][23][24] (Figure 1). Thus, offers the potential to tissue maintenance and regeneration.…”

Section: Introductionmentioning

confidence: 99%

Transdifferentiation potential of adipose-derived stem cells into neural lineage and their application

Ghasemi¹,

Razavi²

2014

J Histol Histopathol

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Adipose-derived stem cells are a kind of mesenchymal stem cells which have a higher frequency in the adipose tissue and can be harvested by minimally invasive procedures. These cells are able to differentiate into other cells outside their lineage such as neuron, neurotrophic factor secreting cells and Schwann cells. Many of the identified neurotrophic factors such as brain-derived neurotrophic factor, nerve growth factor, and glial cell line-derived neurotrophic factor can be produced by adipose-derived stem cells. In addition, these cells when differentiated into neurotrophic factor secreting cells are able to secrete a significantly high level of these factors. Neurotrophic factors have a significant role in cellular processes include cell proliferation, differentiation and maturation. This article reviews the in vitro differentiation of adiposederived stem cells into neural lineage cells and clinical application.

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Stability of neural differentiation in human adipose derived stem cells by two induction protocols

Cited by 56 publications

References 62 publications

Conversion of Adipose Tissue-Derived Mesenchymal Stem Cells to Neural Stem Cell-Like Cells by a Single Transcription Factor, Sox2

Conversion of Adipose Tissue-Derived Mesenchymal Stem Cells to Neural Stem Cell-Like Cells by a Single Transcription Factor, Sox2

Dedifferentiated fat cells: A cell source for regenerative medicine

Transdifferentiation potential of adipose-derived stem cells into neural lineage and their application

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