Stability of latanoprost in an ophthalmic lipid emulsion using polyvinyl alcohol

Simple, fast, and sensitive isocratic high-performance liquid chromatography method for the quantification of latanoprost

“…Morgan et al is the first to measure 12.2 μg mL −1 of latanoprost (minimum concentration) using this method but gave no information about RT [15]. Sakai et al also used this method to analyze latanoprost but neither the lowest concentration measured nor RT has been reported [16]. Varma et al determined 16.44 ± 10.34 μg mL −1 as the minimum concentration of latanoprost with RT of 6-9 min in his study [17].…”

“…Mobile phases such as a mixture of acetonitrile and potassium dihydrogen phosphate (pH 3.0) [18] acetonitrile and sodium octane sulfate (pH 3.0) [16] water, acetonitrile, and methanol containing 0.03% TFA [20] water and methanol with and without THF; water and acetonitrile containing 0.1% TFA (at pH 3.0) with varied compositions were evaluated, but the separation was not as good as obtained with acetonitrile and water (30:70 v/v, pH 3) containing 0.1% TFA (Fig. 2).…”

“…These analytical techniques are: high-performance liquid chromatography (HPLC) equipped with radioactive detectors [8][9][10][11][12][13], diode array detector [14], and UV detector [15][16][17][18][19][20]. The HPLC-UV is the simplest method for the quantification of latanoprost [19] but has longer analysis time (≥3.3 min).…”

Novel NSAIDs ophthalmic formulation: Flurbiprofen axetil emulsion with low irritancy and improved anti-inflammation effect

“…In the last decade, oil-in-water (o/w) type lipid emulsions have been investigated and are now exploited commercially as a vehicle to improve the ocular bioavailability of lipophilic drugs (Tamilvanan et al, 2002;Vandamme, 2002;Yamaguchi et al, 2005;Liu et al, 2009). The natural biodegradability, nanometer droplet size range, sterilizability and substantial drug solubilization either at the innermost oil phase or at the o/w interface, and improved ocular bioavailability are thus making the lipid emulsion a promising ocular delivery vehicle (Lv et al, 2005;Sakai et al, 2005;Tamilvanan and Benita, 2004).…”

The stabilization mechanism of latanoprost

“…Prostaglandin derivatives are likely to degrade in aqueous solutions and adsorb to containers (Morishima et al, 2002;Sakai and Ohtori, 2005). Temperature-dependent reduction of the latanoprost content, a therapeutic drug for glaucoma, has also been reported (Morgan et al, 2001), but, to our knowledge, there has been no report on its mechanism.…”