This study is a retrospective study design to examine the outcome of babies born to HIVpositive mothers in Specialist Hospital Yola, Adamawa state, Nigeria. The total population of women who tested HIV-positive and accessed antenatal care, intra-natal care and post natal care and were enrolled for PMTCT at Specialist Hospital, Yola between January 2013 and December 2013 and infants below 24 months .The objective of this study is to determine the number of the exposed infants that received PCR service using DBS as compared with the total number of positive pregnant mothers and also to identify the gaps with the view to correcting them Total ANC attendee was forty thousand three hundred and Seventy four (40,374) pregnant women of which Eleven thousand three hundred (11,300) received counseled, tested and received results for HIV. Two hundred and Nineteen (219) 1.9% tested were reported positive. This makes up 1.9% of the population tested. The HIV testing among HIV-exposed Infants within the health care facility were (133) 60.7%. The prevalence of HIV infection among HIV-exposed infants who were tested by HIV-1 DNA PCR method was (8) 8.4%. 95 (71%) of the total DBS sample collected had their results. 38 (28.6%) of the infants never received their result.The facility had intermittent supply of commodities for EID of HIV, trained human resources, system of identification of HIV-exposed infants. Early HIV testing during Pregnancy, PMTCT ARV prophylaxis, disclosure of HIV status, enrollment to care and treatment, frequent attendance to EID services, co-trimoxazole prophylaxis and exclusive breast feeding was found to be significant predictors for testing of HIV-exposed infants.