Stability of C-Banded and C-Bandless microchromosomes in clonal sublines of the RVP3 mouse tumor grown serially in vivo

Sainerová, H; Svoboda, Jan

doi:10.1016/0165-4608(81)90063-7

Cited by 4 publications

(4 citation statements)

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“…Stat3 activation leads to the transcriptional regulation of cyclin D1 and c-Myc. The expression of these genes results in the stimulation of proliferative processes [40,67,68]. We observed significant down-regulation of src genes and src-dependent pathway genes (v-myc and stat3) after the treatment of animals with the complex of DDMC/a-miR-155, piR-30074 and miR-125b.…”

Section: Discussionmentioning

confidence: 95%

“…Split ratio was 1:3–1:6. After 5–7 days of cultivation, growing cells were harvested for inoculation in mice [40]. These cells have the possibility to produce primary mouse sarcoma in 100% of cases, and the number of transformation-defective cells is minimal compared with that of other RSV cell line strains [[41], [42], [43], [44]].…”

Section: Methodsmentioning

confidence: 99%

“…These cells have the possibility to produce primary mouse sarcoma in 100% of cases, and the number of transformation-defective cells is minimal compared with that of other RSV cell line strains [[41], [42], [43], [44]]. The Prague strain of RSV is competent for viral replication and transforms mammalian cells but does not produce virus [40]. The cell line was purchased from the Russian Academy of Sciences (RAS) (MWIIW, Moscow) by SID ALEX GROUP, Ltd. (Prague, Czech Republic).…”

Section: Methodsmentioning

confidence: 99%

“…Animals were injected subcutaneously in the interscapular region with an optimal concentration of 5 × 10 4 of cells in 1 ml of culture medium (DMEM, 10% FCS, and an antibiotic mixture of 100 μg/ml penicillin-streptomycin and 32 μg/ml gentamicin). The tumors were removed aseptically, freed of necrotic material, minced with scissors and frozen in culture medium with 20% DMSO [40]. Spleen, lungs, kidneys, heart, blood, liver, and enlarged lymph nodes were washed with sterile PBS, cut into small pieces and frozen in culture medium with 20% DMSO for subsequent RT-PCR and immunofluorescence experiments.…”

Section: Methodsmentioning

confidence: 99%

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The full recovery of mice (Mus Musculus C57BL/6 strain) from virus–induced sarcoma after treatment with a complex of DDMC delivery system and sncRNAs

Klimenko¹,

Сидоров²

2019

Non-coding RNA Research

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Background Virus-induced cellular genetic modifications result in the development of many human cancers. Methods In our experiments, we used the RVP3 cell line, which produce primary mouse virus-induced sarcoma in 100% of cases. Inbreed 4-week-old female C57BL/6 mice were injected subcutaneously in the interscapular region with RVP3 cells. Three groups of mice were used. For treatment, one and/or two intravenous injections of a complex of small non-coding RNAs (sncRNAs) a-miR-155, piR-30074, and miR-125b with a 2-diethylaminoethyl-dextran methyl methacrylate copolymer (DDMC) delivery system were used. The first group consisted of untreated animals (control). The second group was treated with one injection of complex DDMC/sncRNAs (1st group). The third group was treated with two injections of complex DDMC/sncRNAs (2nd group). The tumors were removed aseptically, freed of necrotic material, and used with spleen and lungs for subsequent RT-PCR and immunofluorescence experiments, or stained with Leishman-Romanowski dye. Results As a result, the mice fully recovered from virus-induced sarcoma after two treatments with a complex including the DDMC vector and a-miR-155, piR-30074, and miR-125b. In vitro studies showed genetic and morphological transformations of murine cancer cells after the injections. Conclusions Treatment of virus-induced sarcoma of mice with a-miR-155, piR-30074, and miR-125b as active component of anti-cancer complex and DDMC vector as delivery system due to epigenetic-regulated transformation of cancer cells into cells with non-cancerous physiology and morphology and full recovery of disease.

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Section: Discussionmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The full recovery of mice (Mus Musculus C57BL/6 strain) from virus–induced sarcoma after treatment with a complex of DDMC delivery system and sncRNAs

Klimenko¹,

Сидоров²

2019

Non-coding RNA Research

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DNA Sequence Amplification in Mammalian Cells

Hamlin

Milbrandt²,

Heintz³

et al. 1984

International Review of Cytology

170

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Double minutes in a cell line from mouse fibroblasts grown under nonselective conditions. Suppression of a double minute-free sideline by in vivo environment

Bregula

Levan

2008

Hereditas

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BREGULA. U . and LEVAN. A. 1985. Double minutes in a cell line from niousc fibroblasts grown under nonselective conditions. Suppression of a double minute-free sideline hy in vivo environment. ~ Hereditas 102: 259-276. Lund. Sweden, ISSN 0018-0661. Received September 23,1984 o f t h e mousc. started in serial in vivo culture in January 1983, underwent transformaticin in June into a vigorously growing cell line that had a hypertriploid atemline and a hypertetraploid sideline. The stemline had new markers, some with additional C-bands, and double minutes (DM): the sideline had few markers and no DM. By the end of 1983. the stemline gradually lost all DM and acquired one long homogeneously staining region (HSR). A tumorigenicity test in August 1983 gave rise to a tumor that was subsequently carried in serial culture and studied in frequent fixations. Thc sojourn in thc anlmal host had induced lasting effects on the chromosomes. The most conspicuous effect was total loss of sideline cells and consequent presence of DM in practically all cells. Three problems were taken up for discussion: ( I ) The unexpected formation of DM in a ccll line of normal origin and without known selective pressure;( 2 ) the elimination of all DM-less cells from the tumor, suggesting correlation hctween DM and tumor formation; and (3) the possihly stimulative action on cell viahility of markers with extra C-band\, expressed i n their tendency to accumulate in ccll clones and wmdines. Urszula Btcgiilu, In s/ifiife [I/' Oiicolo~y. Wri wclskn IS, P-00-97.3 Wmwnwa. Poiuird ' On leave from thc Institute o f Oncology. Warszawa. Poland

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Stability of C-Banded and C-Bandless microchromosomes in clonal sublines of the RVP3 mouse tumor grown serially in vivo

Cited by 4 publications

References 9 publications

The full recovery of mice (Mus Musculus C57BL/6 strain) from virus–induced sarcoma after treatment with a complex of DDMC delivery system and sncRNAs

The full recovery of mice (Mus Musculus C57BL/6 strain) from virus–induced sarcoma after treatment with a complex of DDMC delivery system and sncRNAs

DNA Sequence Amplification in Mammalian Cells

Double minutes in a cell line from mouse fibroblasts grown under nonselective conditions. Suppression of a double minute-free sideline by in vivo environment

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