Stability of Antimicrobial Decapeptide (KSL) and Its Analogues for Delivery in the Oral Cavity

Na, Dong Hee; Faraj, Jabar A.; Çapan, Yılmaz; Leung, Kai P.; DeLuca, Patrick P.

doi:10.1007/s11095-007-9274-8

Cited by 47 publications

(46 citation statements)

References 17 publications

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“…They form a chemical barrier to protect the host from bacterial, fungal and viral pathogens, help to control the growth of oral biofilms and may help to reduce the occurrence of dental caries and periodontal disease [12]. Previous studies have shown that KSL can effectively inhibit the growth of several bacterial strains [21,26]. In this study, we tested the antimicrobial activity of KSL against a panel of major oral pathogenic bacteria and fungi.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro toxicity studies showed that KSL did not induce cell death or compromise the membrane integrity of human gingival fibroblasts [8]. In addition, KSL and its analogue KSL-W possess the stability, adsorption and release characteristics necessary for local delivery to the oral cavity in a chewing gum formulation, thereby serving as a practically useful antiplaque agent [19][20][21]. As mentioned above, previous studies demonstrated that KSL inhibited selected oral and non-oral pathogens as well as oral biofilms [4,18].…”

Section: Introductionmentioning

confidence: 94%

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Effect of the antimicrobial decapeptide KSL on the growth of oral pathogens and Streptococcus mutans biofilm

Liu

Wang

Zhou

et al. 2011

International Journal of Antimicrobial Agents

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Effect of the antimicrobial decapeptide KSL on the growth of oral pathogens and Streptococcus mutans biofilm

Liu

Wang

Zhou

et al. 2011

International Journal of Antimicrobial Agents

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“…Another promising anticaries AMP is a synthetic α-helical antimicrobial decapeptide designated KSL-W, which can selectively destabilize the cell membranes of cariogenic bacteria including S. mutans, S. sobrinus, and L. acidophilus [Na et al, 2007;Leung et al, 2009]. This peptide resits enzymatic degradation in human saliva for 1 h, and the potential use of KSL-W as an antibiofilm agent in a chewing gum formulation has been suggested Na et al, 2007].…”

Section: Ecological Approaches To Caries Preventionmentioning

confidence: 99%

“…This peptide resits enzymatic degradation in human saliva for 1 h, and the potential use of KSL-W as an antibiofilm agent in a chewing gum formulation has been suggested Na et al, 2007]. More recently, a hydroxyapatite-binding AMP was designed, based on the fusion of specific hydroxyapatite-binding heptapeptide (HBP7) with KSL-W, and this bioconjugate was shown to have improved oral retention and antibacterial efficacy [Huang et al, 2016b].…”

Section: Ecological Approaches To Caries Preventionmentioning

confidence: 99%

Ecological Approaches to Dental Caries Prevention: Paradigm Shift or Shibboleth?

Philip

Suneja²,

Walsh³

2018

Caries Res

125

104

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Contemporary paradigms of dental caries aetiology focus on the ecology of the dental plaque biofilm and how local environmental factors can modulate this to cause disease. The crucial role that a healthy oral microbiome plays in preventing caries and promoting oral health is also being increasingly recognized. Based on these concepts, several ecological preventive approaches have been developed that could potentially broaden the arsenal of currently available caries-preventive measures. Many of these ecological approaches aim for long-term caries control by either disrupting cariogenic virulence factors without affecting bacterial viability, or include measures that can enhance the growth of health-associated, microbially diverse communities in the oral microbiome. This paper argues for the need to develop ecological preventive measures that go beyond conventional caries-preventive methods, and discusses whether these ecological approaches can be effective in reducing the severity of caries by promoting stable, health-associated oral biofilm communities.

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“…The stability of GH12 in human saliva was analyzed by reversed-phase HPLC as described [Na et al, 2007], with some modifications. Whole human saliva was collected from 3 healthy volunteers and immediately centrifuged at 4,200 g for 20 min at 4 ° C, and the supernatant was filtered through a 0.22-μm syringe filter.…”

Section: Secondary Structure and Stability Of Gh12mentioning

confidence: 99%

Activity of Synthetic Antimicrobial Peptide GH12 against Oral Streptococci

Fan²,

Lv³

et al. 2016

Caries Res

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Controlling the growth of cariogenic microorganisms such as oral streptococci is an adjunct therapy for caries-active individuals to prevent and treat caries. Here we investigated the antimicrobial activity of the synthetic amphipathic α- helical antimicrobial peptide GH12 (GLLWHLLHHLLH-NH₂) against oral streptococci in vitro. Circular dichroism studies showed that GH12 takes on an α-helical conformation in the presence of membrane-mimicking solvents, and reversed-phase high-performance liquid chromatography studies showed that GH12 remains stable in saliva. The peptide showed bactericidal activity against oral streptococci, with minimum inhibitory concentrations ranging from 6.7 to 32.0 μg/ml. GH12 concentrations 4-fold higher than the minimum bactericidal concentration completely killed oral streptococci within 20 min. Treating oral streptococci with GH12 caused noticeable changes in bacterial viability and morphology based on confocal laser scanning microscopy and scanning electron microscopy. Effects of GH12 on biofilm formation and on viability of mature biofilm were quantified by crystal violet staining and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. GH12 effectively inhibited biofilm formation and metabolic activity in biofilms of oral streptococci, especially S. mutans, S. sobrinus and S. salivarius. These results suggest that GH12 shows rapid and strong antimicrobial activity against oral streptococci in vitro, opening the door to preclinical and clinical studies to explore its potential for caries prevention and treatment.

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Stability of Antimicrobial Decapeptide (KSL) and Its Analogues for Delivery in the Oral Cavity

Abstract: This study suggests that KSL-W could be used as an antiplaque agent in a chewing gum formulation.

Cited by 47 publications

References 17 publications

Effect of the antimicrobial decapeptide KSL on the growth of oral pathogens and Streptococcus mutans biofilm

Effect of the antimicrobial decapeptide KSL on the growth of oral pathogens and Streptococcus mutans biofilm

Ecological Approaches to Dental Caries Prevention: Paradigm Shift or Shibboleth?

Activity of Synthetic Antimicrobial Peptide GH12 against Oral Streptococci

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