Stability of Adenoviral Vectors Following Catheter Delivery

Tsui, Lisa; Zayek, Nathalie; Frey, David; Mello, Christopher; Banik, Gautam G.; Falotico, Robert; McArthur, James G.

doi:10.1006/mthe.2000.0237

Cited by 8 publications

(5 citation statements)

References 9 publications

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“…22 The compatibility between the gene transfer vector and the delivery system is of paramount importance and could play a pivotal role in the success of transferring a research tool to a human clinical application. Biocompatibility studies have shown that stainless steel, nitinol, and polycarbonate are incompatible with Ad vectors 6 whereas polypropylene polymers 6 and nylon-based materials 23 maintain Ad vector infectivity. In vivo studies focusing on Ad vector delivery to the endomyocardium showed that higher efficiency was obtained with use of Stiletto catheters as compared to standard nitinol-stainless steel catheters.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of adenoviral vectors in poly(vinyl chloride) tubing catheters with presence or absence of plasticizer di‐2‐ethylhexyl phthalate

Bilbao¹,

Reay²,

Koppanati³

et al. 2004

J Biomedical Materials Res

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Adenoviral (Ad) vectors feature attractive characteristics for gene therapy of a wide variety of diseases. In many cases, the Ad vector must be administered using catheters and other plastic medical devices. Although poly(vinyl chloride) is one of the most frequently used catheter materials, it is relatively rigid and requires the addition of a plasticizer such as di-2-ethylhexyl phthalate (DEHP) to increase its flexibility. In this study, we demonstrated that exposure to a DEHP-containing catheter decreased the infectivity of Ad vectors but not the total particle number of the vector. Loss of Ad vector infectivity was directly related to the time of exposure to the DEHP-containing catheter, but it was not due to simple leaching of the chemical from the plastic. The loss of Ad vector infectivity could be prevented by preflushing the tube with albumin. Careful consideration of the compatibility between gene therapy vectors and medical delivery devices will be critical to the success of human gene therapy applications.

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Section: Discussionmentioning

confidence: 99%

Biocompatibility of adenoviral vectors in poly(vinyl chloride) tubing catheters with presence or absence of plasticizer di‐2‐ethylhexyl phthalate

Bilbao¹,

Reay²,

Koppanati³

et al. 2004

J Biomedical Materials Res

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show abstract

“…For instance, stainless steel and nitinol-based catheters have been found to be less compatible with adenoviral delivery compared with more inert substances such as polypropylene and Teflon. 75,76 These catheters can yield transduction efficiencies in the range of 5-20%. 77−79 As catheter technology has improved, there has been a growing interest in developing complementary techniques to augment gene transfer efficiency.…”

Section: Cardiovascular Delivery Systemsmentioning

confidence: 99%

The Emerging Role of Gene Therapy in the Treatment of Cardiovascular Diseases

Barbato

Kibbe

Tzeng

2003

Critical Reviews in Clinical Laboratory Sciences

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Cardiovascular disease is the number one source of morbidity and mortality in the United States. Therapies directed at a variety of cardiovascular diseases have blossomed over the last several decades. The advent of gene therapy, first as an intriguing tool, and subsequently with the early successes of gene trials involving the treatment of SCID, led to the development of gene therapy as a potentially exciting and viable therapy in cardiovascular diseases. A variety of novel vector technologies and delivery systems have been developed to more efficiently deliver the gene product to the desired organ or tissue bed. Early clinical trials focused on stimulating angiogenesis. Subsequently, a number of other aspects of cardiovascular disease have been identified as potential targets for gene therapy, including the prevention of restenosis, the prevention and treatment of thrombosis, and the prevention of transplant vasculopathy. With over forty clinical human trials either completed or currently enrolling, cardiovascular gene therapy has proven to be safe and initial results suggest its efficacy.

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“…This hypoglycemic condition in 7B2 nulls is temporarily relieved by delivery of a low dose of 7B2-encoding adenovirus to the pituitary; elevation in blood glucose persisted for about 2 wk. The use of repeated low doses of adenovirus might produce a more profound effect in this regard; alternatively, a different means of delivery of recombinant adenoviral vector could be used, for example a catheter-based intravascular infusion method, to obtain longer-lasting benefits on blood glucose (29). 7B2 null mice exhibit elevated circulating insulin-like material because of islet hypertrophy, but the major processing defect in the pancreas is most likely the inability to cleave proglucagon forms to mature glucagon, a defect shared by the PC2 null (13,28).…”

Section: Hyperglycemic Effect Of 7b2-encoding Adenovirusmentioning

confidence: 99%

Intrapituitary Adenoviral Administration of 7B2 Can Extend Life Span and Reverse Endocrinological Deficiencies in 7B2 Null Mice

et al. 2002

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The prohormone convertase PC2 requires the aid of a helper protein, known as 7B2, for production of active enzyme. Deletion of 7B2 results in a lethal phenotype resembling Cushing's disease. In this study, we have investigated the effect of a single low dose of recombinant adenovirus vector encoding 7B2 and delivered directly to the pituitary of 7B2 nulls on pituitary ACTH, plasma ACTH, corticosterone, alpha MSH and glucose, and survival time. We show that after injection of recombinant adenovirus encoding 27-kDa 7B2 into 7B2 nulls, transgene expression, as measured by RIA for 7B2, exhibits a transient elevation in the pituitary and blood, with a slight but significant elevation of PC2 activity in pituitaries of 7B2 nulls and a drop in the level of circulating ACTH concomitant with a small increase in circulating alpha MSH. The level of circulating blood glucose was increased, and that of corticosterone was decreased. Lastly, slight but significantly prolonged survival times were observed. These data showing partial rescue of 7B2 nulls support the idea that adenoviral administration of 7B2 will represent an effective means to study the role of this interesting neuroendocrine protein on endocrine function in vivo.

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Stability of Adenoviral Vectors Following Catheter Delivery

Cited by 8 publications

References 9 publications

Biocompatibility of adenoviral vectors in poly(vinyl chloride) tubing catheters with presence or absence of plasticizer di‐2‐ethylhexyl phthalate

Biocompatibility of adenoviral vectors in poly(vinyl chloride) tubing catheters with presence or absence of plasticizer di‐2‐ethylhexyl phthalate

The Emerging Role of Gene Therapy in the Treatment of Cardiovascular Diseases

Intrapituitary Adenoviral Administration of 7B2 Can Extend Life Span and Reverse Endocrinological Deficiencies in 7B2 Null Mice

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