Stability of 12 T-helper cell-associated cytokines in human serum under different pre-analytical conditions

Stervbo, Ulrik; Bajda, Sharon; Wehler, Patrizia; Rohn, Benjamin; Streichhahn, Melanie; Temizsoy, Sehriban; Kohut, Eva; Roch, Toralf; Viebahn, Richard; Westhoff, Timm H.; Babel, Nina

doi:10.1016/j.cyto.2020.155044

Cited by 5 publications

(3 citation statements)

References 25 publications

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“…The naive and memory T / B lymphocyte subpopulations were reduced under the effect of gravitational stress, a decrease in the number of basophils and eosinophils was also observed. Only circulating neutrophils that have been increased during parabolic light (Stervbo et al, 2020). Exposure to acute noise stress leads, according to a previous study, to a very signi icant decrease in the total number of leukocytes compared to control and a signi icant improvement in the number of neutrophils positive for phagocytosis of Candida in the group exposed to acute noise stress compared to control.…”

Section: Resultsmentioning

confidence: 71%

Distribution of leukocyte subpopulation among students threatened by failure

Chettoum

Guedri

Djerrou

et al. 2020

ijrps

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Psychoneuroimmunology or the study of the relationships between the brain and the immune system is an area of research that has experienced significant development over the decade. Stress does not appear without consequences on the state of health, the role of fears, emotions and significant constraints in the appearance of organic and mental diseases. In this research, we studied the effect of stress and anxiety during exams at the end of the academic year (2018/2019) on the distribution of leukocyte subpopulations and the immune system, questionnaires has been completed by student volunteers, to estimate the anxio-depressive comorbidities through the (HADS) test during and outside exams, and in the same time we asked them for a blood sample the next morning day to carry out some biological assays (CBC). We also found that stress during exams caused a change in the distribution of different types of white blood cells, a total decrease in white blood cell counts with neutropenia and lymphopenia were found in students during exams compared to controls, and an increase in monocyte and other types of polymorphonuclear levels in students during exams compared to controls. Other tests measuring the effects of stress on specific functions of the immune system can be used.

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Section: Resultsmentioning

confidence: 71%

Distribution of leukocyte subpopulation among students threatened by failure

Chettoum

Guedri

Djerrou

et al. 2020

ijrps

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“… 16 17 With regard to specific cytokines, it is suggested that IFN-γ is significantly reduced by the third thaw cycle, while a slight increase of IL-5 and IL-10 is observed after multiple freeze-thaw cycles. 29 IL-1 and IL-8 have a significant decrease in levels after two freeze-thaw cycles, 30 while IL6, IL10 and IL2 levels are stable when plasma samples undergo three free-thaw cycles. 14 IL-9, CXCL10 and Eotaxin-1 are noted to be the most thermally stable cytokine biomarkers.…”

Section: Discussionmentioning

confidence: 96%

“…In serum samples, multiple freeze-thaw cycles have been shown to have limited effect on cytokine levels, increase levels or decrease levels based on the specific cytokine biomarker 16 17. With regard to specific cytokines, it is suggested that IFN-γ is significantly reduced by the third thaw cycle, while a slight increase of IL-5 and IL-10 is observed after multiple freeze-thaw cycles 29. IL-1 and IL-8 have a significant decrease in levels after two freeze-thaw cycles,30 while IL6, IL10 and IL2 levels are stable when plasma samples undergo three free-thaw cycles 14.…”

Section: Discussionmentioning

confidence: 99%

Evaluating the long-term biological stability of cytokine biomarkers in ocular fluid samples

Felfeli,

Park,

Nestor

et al. 2023

BMJ Open Ophth

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PurposeThe quality of biological fluid samples is vital for optimal preanalytical procedures and a requirement for effective translational biomarker research. This study aims to determine the effects of storage duration and freeze-thawing on the levels of various cytokines in the human aqueous humour and vitreous samples.Methods and analysisHuman ocular aqueous humour and vitreous samples were obtained from 25 eyes and stored at −80°C for analysis. All samples were assayed for 27 cytokine biomarker concentrations (pg/mL) using a multiplex assay. Four sample storage durations following sample collection were evaluated (1 week, 3 months, 9 months and 15 months). Additionally, samples underwent up to three freeze-thaw cycles within the study period.ResultsAmong the 27 cytokine biomarkers, concentrations of four cytokines (Interleukin (IL)−2, IL-10, IL-12 and platelet-derived growth factor-BB) were significantly decreased by storage duration at all time points, as early as 3 months following sample collection (range of 9%–37% decline between 1 week and 15 months, p<0.001). Freeze-thawing of up to three cycles did not significantly impact the cytokine biomarker concentrations in aqueous humour or vitreous. Separability of patient-specific cytokine biomarker profiles in the principal component analysis remained relatively the same over the 15 months of storage duration.ConclusionThe findings from this study suggest that several intraocular cytokine biomarkers in human aqueous humour and vitreous samples may be susceptible to degradation with long-term storage, as early as 3 months after collection. The overall patient-specific cytokine biomarker profiles are more stable than concentrations of individual cytokines. Future studies should focus on developing guidelines for optimal and standardised sample handling methods to ensure correct research findings about intraocular biomarkers are translated into clinical practice.

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Clinical utility of measuring CD4⁺T follicular cells in patients with immune dysregulation

LaBere,

Nguyen,

Habiballah

et al. 2023

Preprint

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Mechanistic studies of autoimmune disorders have identified circulating T follicular helper (cTfh) cells as drivers of autoimmunity. However, the quantification of cTfh cells is not yet used in clinical practice due to the lack of age stratified normal ranges and the unknown sensitivity and specificity of this test for autoimmunity. We enrolled 238 healthy participants and 130 patients with common and rare disorders of autoimmunity or autoinflammation. Patients with infections, active malignancy, or any history of transplantation were excluded. In 238 healthy controls, median cTfh percentages (range 4.8% to 6.2%) were comparable among age groups, sexes, races, and ethnicities, apart from a significantly lower percentages in children less than 1 year of age (median 2.1%, CI: 0.4% to 6.8, p<0.0001). Among 130 patients with over 40 immune regulatory disorders, a cTfh percentage exceeding 12% had 88% sensitivity and 94% specificity for differentiating disorders with adaptive immune cell dysregulation from those with predominantly innate cell defects. This threshold had a sensitivity of 86% and specificity of 100% for active autoimmunity and normalized with effective treatment. cTfh percentages exceeding 12% distinguish autoimmunity from autoinflammation, thereby differentiating two endotypes of immune dysregulation with overlapping symptoms and different therapies.

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Stability of 12 T-helper cell-associated cytokines in human serum under different pre-analytical conditions

Cited by 5 publications

References 25 publications

Distribution of leukocyte subpopulation among students threatened by failure

Distribution of leukocyte subpopulation among students threatened by failure

Evaluating the long-term biological stability of cytokine biomarkers in ocular fluid samples

Clinical utility of measuring CD4⁺T follicular cells in patients with immune dysregulation

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Stability of 12 T-helper cell-associated cytokines in human serum under different pre-analytical conditions

Cited by 5 publications

References 25 publications

Distribution of leukocyte subpopulation among students threatened by failure

Distribution of leukocyte subpopulation among students threatened by failure

Evaluating the long-term biological stability of cytokine biomarkers in ocular fluid samples

Clinical utility of measuring CD4+T follicular cells in patients with immune dysregulation

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Clinical utility of measuring CD4⁺T follicular cells in patients with immune dysregulation