Stability-Indicating Spectrophotometric and Densitometric Methods for Determination of Aceclofenac

Abstract

Three methods were developed for the determination of aceclofenac in the presence of its degradation product, diclofenac. In the first method, third-derivative spectrophotometry (D3) is used. The D3 absorbance is measured at 283 nm where its hydrolytic degradation product diclofenac does not interfere. The suggested method shows a linear relationship in the range of 4-24 micrograms mL-1 with mean percentage accuracy of 100.05 +/- 0.88. This method determines the intact drug in the presence of up to 70% degradation product with mean percentage recovery of 100.42 +/- 0.94. The second method depends on ratio-spectra first-derivative (RSD1) spectrophotometry at 252 nm for aceclofenac and at 248 nm for determination of degradation product over concentration ranges of 4-32 micrograms mL-1 for both aceclofenac and diclofenac with mean percentage accuracy of 99.81 +/- 0.84 and 100.19 +/- 0.72 for pure drugs and 100.17 +/- 0.94 and 99.73 +/- 0.74 for laboratory-prepared mixtures, respectively. The third method depends on the quantitative evaluation of thin-layer chromatography of aceclofenac using chloroform:methanol: ammonia (48:11.5:0.5 v/v/v) as a mobile phase. Chromatograms were scanned at 274 and 283 nm for aceclofenac and diclofenac, respectively. The method determined aceclofenac and diclofenac in concentration ranges of 2-10 and 1-9 micrograms spot-1 with mean percentage accuracy of 100.20 +/- 1.03 and 100.14 +/- 0.98 for pure drugs and 99.77 +/- 0.74 and 100.07 +/- 0.78 for laboratory-prepared mixtures, respectively. This method retains its accuracy in the presence of up to 80% degradation product for the studied drug. The suggested procedures were checked using laboratory-prepared mixtures and were successfully applied for the analysis of their pharmaceutical preparation. The validity of the proposed methods was further assessed by applying a standard addition technique. The obtained results agreed statistically with those obtained by the reported method.

Simultaneous densitometric TLC analysis of aceclofenac, paracetamol, and chlorzoxazone in tablets

“…1. Literature survey revealed various analytical methods, for example spectrophotometric, colorimetric, HPLC, and stability-indicating HPTLC methods for analysis of ACF alone and in combination with other drugs in pharmaceutical formulations [8][9][10][11][12][13][14][15][16]. A variety of methods have been used for analysis of PCM [17][18][19][20][21][22].…”

Factorial Design and Machine Learning Strategies: Impacts on Pharmaceutical Analysis

“…This approach depends on differentiation of the regular absorption spectrum using arithmetical transformation into a first-order derivative or a higher order derivative. Several advantages are achieved using DS including but not limited to an improvement in resolution, reduction of noise level, elimination of interferences, augmentation of sensitivity and selectivity, and accordingly an improvement in separation efficiency [51][52][53][54].…”

Application of a Validated Stability-Indicating HPTLC Method for Simultaneous Estimation of Paracetamol and Aceclofenac and their Impurities

“…For ACF methods reported are spectrophotometry [12,13], stability indicating HPLC [14], UPLC [15] and HPTLC [16,17] either alone or in combination with other drugs. It was further revealed that there are analytical methods reported for simultaneous estimation of PCT and ACF like Spectrophotometry [18], HPLC [19][20][21], HPTLC [22], stability indicating HPLC [23] while few reported methods like HPLC [24], HPTLC [25] and stability indicating HPTLC [26] involve simultaneous estimation of PCT and ACF combined with one more additional drug.…”