Stability Indicating Reverse Phase HPLC Method for Estimation of Rifampicin and Piperine in Pharmaceutical Dosage Form

Shah, Umang; Patel, Samir; Raval, Manan

doi:10.2174/1570163814666170619092224

Cited by 10 publications

(4 citation statements)

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“…Quercetin has appeared to build bioavailability, blood levels and adequacy of various medications, including diltiazem, digoxin, verapamil, etoposide, and paclitaxel [13][14][15]. According to the literature, numerous methods, including UV spectroscopy [16,17], RP-HPLC [18][19][20], HPTLC [21,22] and others, are available for determining RIF both alone and in conjunction with various drugs in a variety of dose forms. A few methods, including HPLC [23][24][25][26], UV spectroscopy [27] and HPTLC [28][29][30][31], have been taken into consideration for the assessment of QUE both alone and in conjunction with different drugs in different forms of dosage.…”

Section: Introductionmentioning

confidence: 99%

Chemometric-Assisted UV-Spectrophotometric Method for Quantification of Novel Anti-Tubercular Liquisolid Formulation

Tandel¹,

Patel²,

Thakkar³

et al. 2023

Int J Pharm Res Allied Sci

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To simultaneously determine quercetin and rifampicin in raw material and liquid-solid formulation, a numerical technique built on spectroscopic data associated with partial least squares (PLS) multi -variate measurement is suggested in this work. Rifampicin and quercetin spectra were captured at concentrations that fell within the 2-10 µg/ml linear range for each compound. They were used to compute 25 simulated mixtures containing 16 calibration and 9 validation sets, with wavelength spacing of λ=15 nm in hydrochloric acid (0.1 M) and phosphate buffer pH-6.8. The wavelength ranges were 200 to 630 nm. Based on the validation and calibration data's (RMSE) root mean square errors, the models' appropriateness was determined. Recovery studies (%) and relative prediction errors were used to compare and contrast the analytical capabilities of various chemometric techniques. The recovery study's findings showed that this technique was successfully used to formulate pharmaceuticals without excipient interference. The suggested technique is quick, easy to use, and can be utilised as a replacement for traditional analytical tools in the formulation and quality control of pharmaceuticals.

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Section: Introductionmentioning

confidence: 99%

Chemometric-Assisted UV-Spectrophotometric Method for Quantification of Novel Anti-Tubercular Liquisolid Formulation

Tandel¹,

Patel²,

Thakkar³

et al. 2023

Int J Pharm Res Allied Sci

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“…Literature reported uncovers different HPTLC, HPLC, UPLC, LC-MS/MS, and UPLC-MS/MS strategies that are accessible for the estimation of Rifampicin exclusively and in combination with other drugs in various dosage forms [8][9][10][11][12][13][14][15][16][17]. A few techniques have been accounted for, like HPLC, HPTLC, LC-MS/MS, and UPLC-MS/MS methods for the estimation of Quercetin exclusively and in combination with other drugs in various dosage forms [18][19][20][21][22][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Validated high‐performance thin layer chromatographic method for simultaneous quantification of rifampicin and quercetin in liquisolid formulation using fractional factorial design in robustness study

Tandel

Patel

Thakkar

2022

Separation Science Plus

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The present research study focuses on the development of the high‐performance thin‐layer chromatographic method using the design of experiment approach for the simultaneous estimation of Rifampicin and Quercetin. Chromatographic separation was performed on Aluminium plates precoated with silica gel 60 F254 using chloroform:methanol:formic acid:ethyl acetate:benzene (4:1.8:0.3:1.7:1, v/v/v/v/v) as optimized mobile phase. A fractional factorial design was applied for the robustness study and the independent variables selected were mobile phase composition (A), solvent front (B), chamber saturation time (C), and wavelength (D). It was statistically revealed that the volume of ethyl acetate affected the Rf of both drugs resulting in stricter control of ethyl acetate volume compared to the other three variables. Rf for Rifampicin and Quercetin was 0.35 and 0.71 at 347 nm. The linear concentration range was 100–600 ng/band for both drugs. The %recovery ranged between 95.25% and 96.71% for Rifampicin and 95.33%–96.86% for Quercetin. The method was validated by determination of linearity, precision, accuracy, and specificity according to Q2(R1) guidelines, and the %relative standard deviation values were less than 2% for both drugs. A simple, accurate, and reproducible high‐performance thin‐layer chromatographic method was developed, for routine quality control testing of pharmaceutical formulation.

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“…Al have reported Development and validation of stabilityindicating RP-HPLC method for estimation of pamabrom in tablets and another stability indicating RP-HPLC method for simultaneous estimation of rifampicin and piperine in pharmaceutical dosage form. Different stress conditions were used in study which includes, Hydrolytic decomposition: Acidic and alkaline hydrolysis were carried out in 0.1M HCl and 0.1M NaOH for 50°C for 15 min, Neutral decomposition: Neutral hydrolysis was carried out in distilled water at a 55°C for 30 min, Solutions for oxidative stress studies were prepared using 3% H2O2 at 40°C for 30 min, Photo degradation studies were carried out by exposing the drug to UV light in the UV chamber at the wavelength (λ = 254nm) for 120 min, Thermal studies were also conducted on solid drug, which was heated at 80°C for 30 min in hot air oven [26,27].…”

Section: Introductionmentioning

confidence: 99%

Application of Response Surface Methodology in Development and Optimization of Stability Indicating RP-HPLC Method for Determination of Tolvaptan in Bulk and Formulation

Sutar

Magdum²

2021

JPRI

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Design of Experiment assisted stability indicating RP-HPLC was developed and optimized using response surface methodology for determination of Tolvaptan. Mobile phase was developed and optimized using Design of Experiment with response surface methodology. Acetonitrile and phosphate buffer with pH 5.5 (70:30% V/V) was optimized as mobile phase. The flow rate was maintained at 1ml/min. Stress studies were performed as per guidelines. Method was validated in accordance with regulatory requirements and results were within specified limits of regulatory guidelines. Tolvaptan was eluted at 3.24 min. It shows linearity from 2.5-15 µg/ml. Coefficient of correlation was 0.999, LOD and LOQ values were 1.0871 (µg/ml) and 3.2942 (µg/ml). Precision was determined with % RSD of 0.8669 and 0.9709%, mean percentage Recovery value was found to be 99.88 ±1.2. All stress degradation products are very well resolved from drug peak which indicate suitability indicating nature of the developed method. Design of Experiment technique can help in fast and economical optimization of mobile phase which inturn will save time for method development. The developed method is simple, accurate, sensitive which can be utilised as stability indicating method for identification of degradation products in routine analysis of the drug.

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Stability Indicating Reverse Phase HPLC Method for Estimation of Rifampicin and Piperine in Pharmaceutical Dosage Form

Cited by 10 publications

References 0 publications

Chemometric-Assisted UV-Spectrophotometric Method for Quantification of Novel Anti-Tubercular Liquisolid Formulation

Chemometric-Assisted UV-Spectrophotometric Method for Quantification of Novel Anti-Tubercular Liquisolid Formulation

Validated high‐performance thin layer chromatographic method for simultaneous quantification of rifampicin and quercetin in liquisolid formulation using fractional factorial design in robustness study

Application of Response Surface Methodology in Development and Optimization of Stability Indicating RP-HPLC Method for Determination of Tolvaptan in Bulk and Formulation

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