Stability indicating HPLC method for simultaneous determination of mephenesin and diclofenac diethylamine

Abstract

A simple, specific, accurate and stability-indicating reversed phase high performance liquid chromatographic method was developed for the simultaneous determination of mephenesin and diclofenac diethylamine, using a Spheri-5-RP-18 column and a mobile phase composed of methanol: water (70:30, v/v), pH 3.0 adjusted with o-phosphoric acid. The retention times of mephenesin and diclofenac diethylamine were found to be 3.9 min and 14.5 min, respectively. Linearity was established for mephenesin and diclofenac diethylamine in the range of 50-300 μg/ml and 10-60 μg/ml, respectively. The percentage recoveries of mephenesin and diclofenac diethylamine were found to be in the range of 99.06-100.60% and 98.95-99.98%, respectively. Both the drugs were subjected to acid, alkali and neutral hydrolysis, oxidation, dry heat, photolytic and UV degradation. The degradation studies indicated, mephenesin to be susceptible to neutral hydrolysis, while diclofenac diethylamine showed degradation in acid, H2O2, photolytic and in presence of UV radiation. The degradation products of diclofenac diethylamine in acidic and photolytic conditions were well resolved from the pure drug with significant differences in their retention time values. This method can be successfully employed for simultaneous quantitative analysis of mephenesin and diclofenac diethylamine in bulk drugs and formulations.

Stabilization of Deformable Nanovesicles Based on Insulin-Phospholipid Complex by Freeze-Drying

“…Different reconstituted solvents, considerably influence the reconstituted process of deformable nanovesicles. The addition of ions to micro-particles usually has a demulsification effect, so the addition of ions might disrupt the integrity of deformable nanovesicles [36]. In order to explore the effect of ions on the reconstitution of deformable nanovesicles, we included the zeta potential of reconstituted nanovesicles.…”

Permeability Evaluation Through Chitosan Membranes Using Taguchi Design

“…Stirring of receptor fluid was performed at 600 rpm and at 37±05 °C. Aliquots (0.5 ml) were withdrawn at various intervals and immediately analyzed for diclofenac diethylamine by HPLC (Agilent Technologies 1200 series, Germany) using eclipse XDB-C 18 column (4.6×150 mm), and UV detector (Dual wavelength) at 221nm by modifying the method as reported by Mulgund et al, [ 11 ]. A mixture of methanol and water (70:30) at flow rate of 1.0 ml min −1 was used as mobile phase for diclofenac diethylamine.…”