Stability-indicating HPLC-DAD method for the simultaneous determination of fluoroquinolone in combination with a non-steroidal anti-inflammatory drug in pharmaceutical formulation

Abstract

We developed and validated a stability-indicating assay method for the simultaneous determination of enrofloxacin and piroxicam in combination and in the presence of degradation products. Reversephase high-performance liquid chromatography analyses were carried out on a Vertisep C18 column and acetonitrile-water (48:52 v/v, pH 3.0) mobile phase with a 1.00 mL min −1 flow rate. The efficient chromatographic separation of these drugs and their forced degradation products was achieved in less than 5 min with a peak purity match factor higher than 950. The method used showed linearity in the concentration ranges of 0.25 to 16.0 μg mL −1 for enrofloxacin (r = 0.9997) and 0.125 to 8.0 μg mL −1 for piroxicam (r = 0.9999) as well as precision (relative standard deviation lower than 2%), accuracy (mean recovery 100 ± 2%), and robustness, according to ICH (International Conference on Harmonization) and AOAC (Association of Official Analytical Chemists) guidelines. This method can simultaneously determine the combination of these drugs in a veterinary formulation and separate the drug peaks from their forced degradation products. Additionally, its optimized chromatographic conditions can contribute to the quality control of this formulation in pharmaceutical manufacturing plants and minimize waste from the organic solvent.

Choices of chromatographic methods as stability indicating assays for pharmaceutical products: A review

“…In addition, Araujo et al. (2020) had successfully developed and validated the method for simultaneous determination of enrofloxacin and piroxicam and their respective degradation products in veterinary formulations.…”

Fabrication of PVC Enrofloxacin-Selective Electrodes for Estimating Enrofloxacin in Pure Form and as Preparation Formula

“…1 shows the chemical structure of enrofloxacin. The literature has revealed several logical strategies for quantitative assessment of enrofloxacin by spectrophotometry [7][8][9] , HPLC 10,11 , fluorescence 12,13 , and electrochemical technique 14 . The aim of this study is to foster an original particle-specific terminal method to determine enrofloxacin ionselective electrodes in drug measurement.…”