Stability in the HIV vDNA pool in peripheral CD4+ T cells of untreated patients by single tube quantitative PCR

Hockett, Richard D.; Saag, Michael S.; Kilby, J. Michael; Sfakianos, Greg; Wakefield, T. B.; Bucy, R. P.

doi:10.1016/s0166-0934(00)00139-7

Cited by 8 publications

(6 citation statements)

References 31 publications

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“…The predictive value of HIV‐DNA was found to be higher than that of plasma HIV‐RNA and CD4 cell counts [33]. In two studies of the longitudinal HIV‐DNA profiles, proviral loads were found to be stable [34, 35]. Hence, the HIV‐DNA level might be established early in infection and then remain stable for years.…”

Section: Discussionmentioning

confidence: 99%

Virological and immunological profiles among patients with undetectable viral load followed prospectively for 24 months

et al. 2003

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Objective To quantify HIV‐RNA in plasma, in lymphoid tissue and proviral DNA in peripheral blood mononuclear cells and to relate these to immunological markers among patients with plasma viral load counts of ≤ 200 HIV‐RNA copies/mL. Methods A prospective study of one hundred and three patients was undertaken with an inclusion criteria of plasma viral load of ≤ 200 copies/mL. The patients had advanced HIV infection; 25% had developed AIDS. Patients were seen every 6 months for a period of 2 years. Results The median plasma viral load was < 20 copies/mL with no increase during follow‐up. Thirty‐one per cent had plasma viral load of ≤ 20 copies/mL at all visits, 44% had ≥ 1 measurement with 21–200 and 25% had ≥ 1 sample with plasma HIV‐RNA > 200 copies/mL. Lymphoid tissue viral load was low at enrolment and declined further during follow‐up. Baseline HIV‐DNA and immunoglobulin (IgA) differed significantly between the plasma viral load rebound groups (P < 0.05). Conclusion In this cohort, selected solely on the basis of having a plasma viral load of ≤ 200 copies/mL, we found stable or declining viral loads in the measured compartments during 2 years of follow‐up. Baseline HIV‐DNA and IgA levels were higher among patients with less complete virological suppression relative to patients with persistently undetectable plasma HIV‐RNA. Hence, a high cellular level of HIV‐DNA and high plasma IgA may predict subsequent development of low‐grade viraemia.

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Section: Discussionmentioning

confidence: 99%

Virological and immunological profiles among patients with undetectable viral load followed prospectively for 24 months

et al. 2003

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“…But data regarding the decline in DNA are sometimes conflicting. Some authors noted decreased levels after one year of antiretroviral triple combination therapy [12] and others reported stable HIV-1 DNA levels over several years in PI-ART naive patients [13,14]. Several assays for the quantification of HIV type 1 proviral DNA in peripheral blood mononuclear cells (PBMC) have previously been reported, and many of them are based either on the principle of conventional PCR requiring post PCR analysis or on real-time PCR on total PBMC, using specific probe detection [9,10,15-23].…”

Section: Introductionmentioning

confidence: 99%

Human immunodeficiency virus type 1 (HIV-1) proviral DNA load in purified CD4+ cells by LightCycler® Real-time PCR

et al. 2005

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Background: The human immunodeficiency virus type 1 (HIV-1) proviral DNA persists in infected cells, even after prolonged successful HAART. In the present study, a relative quantification assay of HIV-1 proviral DNA by LightCycler ® real-time PCR based on SYBR Green I detection was developed in comparison to the number of purified CD4+ cells as estimated by the quantification of the β-globin gene.

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“…However, viral replication persists, as suggested by ongoing viral sequence evolution (14,43), the presence of two-long-terminal-repeat episomal circles (36), the expression of viral mRNA in lymphoid cells and peripheral blood mononuclear cells (PBMCs) (16), the persistence of proviral DNA (10,17), and the presence of low but detectable levels of viral mRNA and infectious virions in the plasma of infected subjects (9,10,42). Depending on the magnitude of residual viral replication during HAART, viral sequence evolution and drug resistance may result, thereby contributing to disease progression (24).…”

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confidence: 99%

Residual Viral Replication during Antiretroviral Therapy Boosts Human Immunodeficiency Virus Type 1-Specific CD8⁺T-Cell Responses in Subjects Treated Early after Infection

Ortiz

Goldwitz

et al. 2002

J Virol

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Human immunodeficiency virus type 1 (HIV-1)-infected subjects treated early after infection have preserved HIV-1-specific CD4؉ T-cell function. We studied the effect of highly active antiretroviral therapy (HAART) on the frequency of HIV-1-specific CD8 ؉ T cells in patients treated during early (n ‫؍‬ 31) or chronic (n ‫؍‬ 23) infection. The degree of viral suppression and time of initiation of treatment influenced the magnitude of the CD8 ؉ T-cell response. HIV-1-specific CD8 ؉ T cells can increase in number after HAART in subjects treated early after infection who have episodes of transient viremia.

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Stability in the HIV vDNA pool in peripheral CD4+ T cells of untreated patients by single tube quantitative PCR

Cited by 8 publications

References 31 publications

Virological and immunological profiles among patients with undetectable viral load followed prospectively for 24 months

Virological and immunological profiles among patients with undetectable viral load followed prospectively for 24 months

Human immunodeficiency virus type 1 (HIV-1) proviral DNA load in purified CD4+ cells by LightCycler® Real-time PCR

Residual Viral Replication during Antiretroviral Therapy Boosts Human Immunodeficiency Virus Type 1-Specific CD8⁺T-Cell Responses in Subjects Treated Early after Infection

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Stability in the HIV vDNA pool in peripheral CD4+ T cells of untreated patients by single tube quantitative PCR

Cited by 8 publications

References 31 publications

Virological and immunological profiles among patients with undetectable viral load followed prospectively for 24 months

Virological and immunological profiles among patients with undetectable viral load followed prospectively for 24 months

Human immunodeficiency virus type 1 (HIV-1) proviral DNA load in purified CD4+ cells by LightCycler® Real-time PCR

Residual Viral Replication during Antiretroviral Therapy Boosts Human Immunodeficiency Virus Type 1-Specific CD8+T-Cell Responses in Subjects Treated Early after Infection

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Residual Viral Replication during Antiretroviral Therapy Boosts Human Immunodeficiency Virus Type 1-Specific CD8⁺T-Cell Responses in Subjects Treated Early after Infection