Stability Evaluation and Degradation Studies of DAC® Hyaluronic-Polylactide Based Hydrogel by DOSY NMR Spectroscopy

Abstract

The stability and the degradation of polymers in physiological conditions are very important issues in biomedical applications. The copolymer of hyaluronic acid and poly-D,L-lactic acid (made available in a product called DAC®) produces a hydrogel which retains the hydrophobic character of the poly-D,L-lactide sidechains and the hydrophilic character of a hyaluronic acid backbone. This hydrogel is a suitable device for the coating of orthopedic implants with structured surfaces. In fact, this gel creates a temporary barrier to bacterial adhesion by inhibiting colonization, thus preventing the formation of the biofilm and the onset of an infection. Reabsorbed in about 72 h after the implant, this hydrogel does not hinder bone growth processes. In the need to assess stability and degradation of both the hyaluronan backbone and of the polylactic chains along time and temperature, we identified NMR spectroscopy as a privileged technique for the characterization of the released species, and we applied diffusion-ordered NMR spectroscopy (DOSY-NMR) for the investigation of molecular weight dispersion. Our diffusion studies of DAC® in physiological conditions provided a full understanding of the product degradation by overcoming the limitations observed in applying classical chromatography approaches by gel permeation UV.

Utilização do hidrogel (DAC) na prevenção de infecções em procedimentos com fixação interna estável (FIE): Revisão sistemática da literatura

“…As buscas por referências nas bases de dados não foram restringidas quanto ao tipo de estudo ou quanto ao ano em que foram publicadas, uma vez que o tema abordado pode ser considerado como recente e com escassos estudos em humanos. Dos estudos selecionados, de acordo com os critérios de elegibilidade previamente estabelecidos, somente um possuía um desenho longitudinal com pacientes (Zagra et al 2019); dois eram relatos de casos clínicos (Grillo et al 2020;Pedrosa et al 2021); nove foram feitos in vitro (Casadidio et al 2018;Censi et al 2019;Drago et al 2014;Guan et al 2016;Guzzo et al 2020;Harris et al 2005;Huang et al 2022;Liao et al 2020;Pitarresi et al 2013) e o restante in vivo, utilizando amostras de animais, ratos ou coelhos (ter Boo et al 2016;Ter Boo et al 2018;Boot et al 2020;Boot et al 2017;Giavaresi et al 2014;Gribova et al 2020;Li et al 2020). Nas buscas não foram encontradas revisões sistemáticas, tampouco meta análises.…”

Glycosaminoglycans: Carriers and Targets for Tailored Anti-Cancer Therapy

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Preface for the Special Issue on the Exploration of the Multifaceted Roles of Glycosaminoglycans: GAGs

“…The suitability of such a hydrogel depends on the stability and degradation of both the hyaluronan backbone and the polylactic chains over time and temperature. T. Guzzo and her collaborators [ 18 ] performed chromatographic analysis and explored the suitability of diffusion-ordered NMR spectroscopy to characterize the outcome of the biomaterial over time in physiological conditions.…”