Stability at low temperatures of neuronal microtubules in spinal ganglia and dorsal roots of the lizard (Lacerta muralis)

Pannese, Ennio; Arcidiacono, G.; Rigamonti, L; Procacci, Patrizia; Ledda, M.

doi:10.1016/s0022-5320(82)90049-1

Cited by 16 publications

(5 citation statements)

References 34 publications

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“…There is wealth of information for many other contemporary lines of investigation, such as detailed descriptions of MT behaviors during axon branching , of the ultrastructure of growth cones, dendrites, and synapses (Peters et al, 1991), or of curious phenomena such as impressive packages of ER-derived tubular structures found in certain axons (Andres, 1965b;Peters et al, 1991). Axonal MTs of species as diverse as cockroach, lamprey, frog, toad, chick, mouse, and rats were reported to contain luminal material in form of a ∼4-nm-thick central dot or filament (Andres, 1965a;Burton, 1984Burton, , 1987Gonatas and Robbins, 1965;Lane and Treherne, 1970;Nixon et al, 1994;Rodríguez Echandía et al, 1968;Smith et al, 1970Smith et al, , 1975Wuerker and Palay, 1969), matching recent reports of MTs with incorporated actin filaments or MAP6/stable tubule-only peptide (Cuveillier et al, 2020;Paul et al, 2019 Preprint), of which the latter could help to explain long-term cold resistance of axonal MTs observed in vivo (Delphin et al, 2012;Pannese et al, 1982; but see also Song et al, 2013). Furthermore, classical studies revealed the presence of post-translationally modified subdomains in MT lattices (Baas and Ahmad, 1992;Baas and Black, 1990;Baas and Joshi, 1992), which start finding their explanations in current models of MT stability (Baas et al, 2016).…”

Section: Conclusion and Final Remarkssupporting

confidence: 66%

Cytoskeletal organization of axons in vertebrates and invertebrates

Prokop

2020

Journal of Cell Biology

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The maintenance of axons for the lifetime of an organism requires an axonal cytoskeleton that is robust but also flexible to adapt to mechanical challenges and to support plastic changes of axon morphology. Furthermore, cytoskeletal organization has to adapt to axons of dramatically different dimensions, and to their compartment-specific requirements in the axon initial segment, in the axon shaft, at synapses or in growth cones. To understand how the cytoskeleton caters to these different demands, this review summarizes five decades of electron microscopic studies. It focuses on the organization of microtubules and neurofilaments in axon shafts in both vertebrate and invertebrate neurons, as well as the axon initial segments of vertebrate motor- and interneurons. Findings from these ultrastructural studies are being interpreted here on the basis of our contemporary molecular understanding. They strongly suggest that axon architecture in animals as diverse as arthropods and vertebrates is dependent on loosely cross-linked bundles of microtubules running all along axons, with only minor roles played by neurofilaments.

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Section: Conclusion and Final Remarkssupporting

confidence: 66%

Cytoskeletal organization of axons in vertebrates and invertebrates

Prokop

2020

Journal of Cell Biology

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“…At this point it must be noted that, in contrast t o the results presented here, other studies have claimed that axonal MT appear to be stable to cold (Pannese et al, 1982;Lewis and Burton, 1977). On the other hand, there are reports in agreement with the present report indicating that MT in peripheral nerves are affected by cold (Rodriguez-Echandia and Piezzi, 1968;Banks et al, 1975;Brimijoin et al, 1979).…”

Section: Discussioncontrasting

confidence: 88%

Microtubule stability along mammalian peripheral nerves

Donoso

1986

J. Neurobiol.

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Microtubule (MT) number, axonal area, and MT density were examined in unmyelinated axons of rat cervical vagus nerve. Study of nerve regions proximal (1-5 mm) and distal (35-40 mm) to the nodosum ganglion in controls (incubation at 37 degrees C for 1 h) showed that the number of MT per axon is significantly less in distal than in proximal nerve regions. Cooling (incubation at 0 degree C for 1 h) caused a significant reduction in the number of MT per axon in both nerve regions. The unmyelinated axons from both nerve regions showed a comparable reduction in MT number by cooling, indicating that axonal MT stability to cold was not significantly different between these two nerve regions. In these nerves no detectable changes were found in cross-axonal area of unmyelinated axons between distal and proximal nerve regions. In another experimental series, in distal nerve regions (35-40 mm from the nodosum ganglion) the number of MT was not further reduced in nerves incubated at 0 degree C by increasing the incubation time. Similar results were obtained from colchicine treated nerves (incubation at 37 degrees C, with 10 mM colchicine for 1 and 2 h). Distal nerve regions (35-40 mm from the nodosum ganglion) showed a similar reduction in the number of MT per axon when nerves were incubated at 0 degree C or with colchicine, suggesting that this drug, as well as cold, may be affecting a similar population of axonal MT, i.e., MT susceptible to anti-MT agents. These results indicate that approximately one-half of the axonal MT are stable to cold as well as to colchicine in rat unmyelinated axons.

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“…On the other hand, frog bladder microtubules are resistant to cold: frogs maintained at 4°C for 15 d exhibit, in their bladder granular cells, the same extended network as at 20°C; however, if an isolated bladder, from room temperature-adapted frogs, is refrigerated at 4°C for 4 h, only a few fluorescent fragments of microtubules can be observed, an aspect highly reminiscent of the action of the depolymerizing drugs (unpublished observations). This cold adaptation has also been observed in fish and lizards [21]. It appears in relation to a biochemical modification of the microtubular structure by a proteic or peptidic stabilizing factor [13] or by a special alphatubulin [7].…”

Section: Microtubular Networkmentioning

confidence: 60%

Microtubules and actin microfilaments in the amphibian bladder granular cells

Hugon¹,

Ibarra²,

Valenti

et al. 1989

Biology of the Cell

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Microtubules and microfilaments were localized by an immunocytochemical method in the granular cells of the frog bladder after fixation and isolation. An extensive array of microtubules was observed in the granular cells with an orientation towards the luminal plasma membrane in the supranuclear zone. Actin filaments formed a continuous bundle that underlined the cellular membrane. After incubation in the presence of colchicine, nocodazole, or tubulozole, the microtubular network appeared fragmented but did not disappear completely. These observations are related to the role of the cytoskeleton in the permeability response of the frog bladder epithelium to vasopressin.

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Stability at low temperatures of neuronal microtubules in spinal ganglia and dorsal roots of the lizard (Lacerta muralis)

Cited by 16 publications

References 34 publications

Cytoskeletal organization of axons in vertebrates and invertebrates

Cytoskeletal organization of axons in vertebrates and invertebrates

Microtubule stability along mammalian peripheral nerves

Microtubules and actin microfilaments in the amphibian bladder granular cells

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