Stability and storage of compounds labelled with radioisotopes

Bayly, R. J.; Evans, E. A.

doi:10.1002/jlcr.2590020102

Cited by 71 publications

(18 citation statements)

References 41 publications

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“…24 C-and especially 3 H-labeled compounds are vulnerable to decomposition by self-radiolysis (see review by Bayly and Evans, 1966). Most methods of analysis of the radioactive amino acid are not sensitive enough to detect impurities of less than 1%.…”

Section: Unspecific Binding Of a Mino Acidsmentioning

confidence: 99%

The synthesis and secretion of albumin

Schreiber¹,

Urban

Reviews of Physiology, Biochemistry and Pharmacology

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Section: Unspecific Binding Of a Mino Acidsmentioning

confidence: 99%

The synthesis and secretion of albumin

Schreiber¹,

Urban

Reviews of Physiology, Biochemistry and Pharmacology

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“…Only the use of silver nitrate impregnated plates of silica gel and then only one solvent system resolved the impurities (Fig. 2) (Bayly & Evans, 1968 Because of the weak beta radiation from tritium, self-adsorption problems can arise with chromatograms and care must be taken in the methods which are used to spot the material on the chromatogram. For example, the paper should be air dried to avoid 'transverse' chromatography.…”

Section: E T H O D S O F a N A L Y S I Smentioning

confidence: 99%

Purity and stability of radiochemical tracers in autoradiography

Evans¹

1972

Journal of Microscopy

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SUMMARY Autoradiography is used as a means of ‘visualizing’ the results of tracer investigations; it gives a measure of ‘events’ which have already occurred. This paper discusses some of the problems peculiar to radiochemicals which should be clearly recognized prior to the application of autoradiographic techniques and the interpretation of the data obtained. Many of the problems described in this paper are concerned with tritium compounds which are particularly valuable and widely used for studies involving high resolution autoradiography. The physical (nuclear) properties of tritium, the low maximum beta energy (18 keV) of the radiation and the high maximum specific activity (29 Ci/mg atom of hydrogen), makes tritium the ideal isotope for determining the precise localization of compounds, drugs and hormones for example, in biological specimens. Refinements in autoradiographic techniques which have been developed in recent years have permitted high sensitivity in the detection of the tracer. While the importance of using compounds of known radiochemical purity for any tracer investigation is widely recognized, it is necessary to ensure that the data obtained is related to the radiochemical tracer compound and is not a consequence of the presence of radiochemical impurities. In this paper are therefore described some of the problems which can arise using impure tracer compounds and their effects on the interpretation of autoradiographs. The methods for the determination of purity, for example chromatographic, isotope dilution analysis, autoradiographic and spectroscopic methods are discussed together with some of the difficulties associated with these analytical techniques. The instability of radiochemicals on storage prior to their use is now a well documented phenomenon; it is a particularly severe problem with compounds used in high resolution autoradiographic studies because of the high molar specific activities which are necessary. Factors which determine the rate of self‐decomposition are discussed, such as the molar specific activity of the compounds, the energy of the radiation and the storage conditions selected for the compound. Current methods for minimizing self‐decomposition of radiochemicals are discussed and illustrated by examples. When using tritium compounds, the integrity of the tritium label is often related to the position of the tritium atom(s) in the tracer compound. However, many of the methods used for the preparation of tritium compounds can yield products in which the specificity of the labelling is uncertain. The importance of the specificity of labelling in such tracers in relation to the interpretation of autoradiographic data is therefore also discussed.

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“…For carbon-14 the theoretically possible specific radioactivity is 62.4 mC/m-atom (Bayly and Evans, 1968), but in practice values of the order of 40 mC/mmole are available for 14C compounds. Liquid scintillation counting is obviously the method of choice, and for the problem under consideration it is generally necessary to count aqueous solutions.…”

Section: Labeled Mutagensmentioning

confidence: 99%

Chemical Mutagens

1971

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Stability and storage of compounds labelled with radioisotopes

Cited by 71 publications

References 41 publications

The synthesis and secretion of albumin

The synthesis and secretion of albumin

Purity and stability of radiochemical tracers in autoradiography

Chemical Mutagens

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