Stability and dynamics of enzyme activity patterns in the rice rhizosphere: Effects of plant growth and temperature

Abstract

The rhizosphere is the most dynamic hotspot of microbial activity in the soil. Despite these dynamics, the spatial pattern of many rhizosphere properties may remain stable because they are continuously reproduced in the changing environment. Low substrate concentration can strongly reduce the rate response of an enzymatic reaction subjected to increased temperature and is recognized as a canceling effect on enzyme temperature sensitivity. Carbon input from rhizodeposits affects C availability in the rhizosphere, and thus the enzyme activities responsible for organic matter decomposition, and their temperature sensitivities, upset the dynamics and stability of biochemical processes in the rhizosphere. However, it is unclear whether a canceling effect occurs in the rhizosphere. We studied temperature effects on chitinase and phosphatase during rice (Oryza sativa L.) growth at 18 and 25°C. The spatial distribution of enzyme activities was imaged using soil zymography and showed that the overall activities of these enzymes increased with temperature but decreased with rice growth. The temporal dynamics of hotspot areas were enzyme-specific. During growing days 14-30, hotspot areas decreased from 2-2.5% to 0.3-0.5% for chitinase, but increased from 2% to 6-7% for phosphatase. The distribution pattern of both enzymes shifted from being dispersed throughout the soil to being associated with the roots. For the first time, we showed the extent of rhizosphere enzyme activity in paddy soil and demonstrated that it is temporally stationary and independent of temperature. However, the temperature sensitivity of enzyme activities declined radically (Q 10 ∼1.3-1.4) at the root surface compared to that of bulk soil (Q 10 ∼1). We conclude that the spatio-temporal pattern of rhizosphere enzymatic hotspots is mainly affected by plant growth. High temperature sensitivity (Q 10 > 1) at the root-soil interface for the tested enzymes revealed that warming will lead to faster nutrient mobilization in the rhizosphere than in root-free soil.

Alkaline Phosphomonoesterase-Harboring Microorganisms Mediate Soil Phosphorus Transformation With Stand Age in Chinese Pinus massoniana Plantations

“…Frontiers in Microbiology | www.frontiersin.org P concentrations, according to many previous studies (Sakurai et al, 2008;Veraart et al, 2015;Wei et al, 2019). In addition, P limitation simulates phoD-harboring microorganisms to synthesize and secrete ALP (Santos-Beneit, 2015;Bergkemper et al, 2016;Ge et al, 2017), which mineralizes Po into Pi and, in turn, increases soil P availability.…”

C:N:P stoichiometry regulates soil organic carbon mineralization and concomitant shifts in microbial community composition in paddy soil

“…Enzyme activities were determined following the method of Marx et al (2001). Fluorogenic methylumbelliferonebased artificial substrates were used to estimate the activities of β-1,4-glucosidase, β-N-acetylglucosaminidase, and acid phosphomonoestersae, which are involved in soil organic C, N, and P dynamics, respectively (Ge et al 2017;Nannipieri et al 2011;Sinsabaugh and Follstad Shah 2012). The fluorogenically labeled substrates used were 4-MUB-β-D-glucoside, 4-MUB-N-acetyl-β-D-glucosaminide, and 4-MUBphosphate, respectively.…”

“…Paddy cultivation areas cover 1.53 million km 2 worldwide and play an important role in sequestering a significant amount of soil organic C (SOC) (Ge et al 2017;Lal 2004;Liu et al 2019b). The sequestration of organic C by paddy soils is ongoing due to the incorporation of plant residues, root exudates, or organic fertilizers (Ge et al 2015;Liu et al 2019b;Luo et al 2019;Zhu et al 2017).…”

Wheat Growth-Induced Changes in Phosphorus Pools in the Crop Residue Detritusphere Are Influenced by Residue C/P Ratio

“…The rhizosphere and detritusphere are two of the three hotspots of microbial activity in the soil (Ge et al 2017;Kuzyakov and Blagodatskaya 2015). The rhizosphere is the soil within approximately 2 mm from the root surface (Dotaniya and Meena 2015).…”