“…Traditionally, GA 3 is produced by submerged fermentation by employing G. fujikuroi . Conventional techniques used for determination of GA 3 and other plant hormones include spectrophotometry (), high-performance thin layer liquid chromatography (), gas chromatography ( , ), and high-performance liquid chromatography (HPLC) ( − ). 1 Structure of GA 3 .…”
Micellar electrokinetic chromatography (MEKC) was developed as a method for quantitative determination of gibberellic acid (GA3) in fermentation broth and commercial products, using 25 mM disodium tetraborate as a buffer at pH 9.2 and 100 mM sodium dodecyl sulfate as a micellar phase. The baseline resolution (Rs of GA3 from other compounds in fermentation broth was achieved with Rs > 2.5. The addition of methanol or acetonitrile in the MEKC buffer did not give a better resolution. Advantages of this MEKC method include high accuracy and precision and no sample preparation except for dilution and filtration.
“…Traditionally, GA 3 is produced by submerged fermentation by employing G. fujikuroi . Conventional techniques used for determination of GA 3 and other plant hormones include spectrophotometry (), high-performance thin layer liquid chromatography (), gas chromatography ( , ), and high-performance liquid chromatography (HPLC) ( − ). 1 Structure of GA 3 .…”
Micellar electrokinetic chromatography (MEKC) was developed as a method for quantitative determination of gibberellic acid (GA3) in fermentation broth and commercial products, using 25 mM disodium tetraborate as a buffer at pH 9.2 and 100 mM sodium dodecyl sulfate as a micellar phase. The baseline resolution (Rs of GA3 from other compounds in fermentation broth was achieved with Rs > 2.5. The addition of methanol or acetonitrile in the MEKC buffer did not give a better resolution. Advantages of this MEKC method include high accuracy and precision and no sample preparation except for dilution and filtration.
The production of gibberellic acid (GA3) by Fusarium moniliforme M-7121 in solid-state culture was evaluated in flask cultures as well as in 3-I horizontal rotary reactors. The highest production rate of GA3 was with 80% (w/v) maize flour mixed with wheat bran. The optimum initial moisture content was inversely dependent on the ambient relative humidity. The initial water activity range for optimal growth and GA3 accumulation was about 0.98 to 0.99, which is unusually high for a filamentous fungus. A low O2 concentration resulted in a much decreased GA3 yield and the appearance of a yellow to reddish pigmentation in the mycelium. The lag phase was short and rapid growth continued for up to 2 days in the rotary reactor, with a maximum specific growth rate of 0.12 h(-1). The maximum rate of GA3 production occurred during the subsequent 3 to 10 days of incubation and the final GA3 concentration reached was 18.7 mg to 19.3 mg/g dry culture. The point of maximum GA3 accumulation after 10 to 12 days of incubation was usually marked by a sharp increase in pH.
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